Characterization of DNA degradation using direct current conductivity and dynamic dielectric relaxation techniques

AAPS PharmSciTech

Volumn 7, Issue 2, 2006, Pages

  Sheu, Jonathan I ^a   Sheu, Eric Y ^b  

^a Acalanes High School   (United States)

^b NONE   (United States)

Author keywords

Conductivity; Degradation; Dielectric relaxation; DNA

Indexed keywords

ALKALINE PHOSPHATASE; HUMAN GROWTH HORMONE; PLASMID DNA;

ARTICLE; CONDUCTANCE; CONTROLLED STUDY; DILUTION; DIRECT CURRENT; DNA DEGRADATION; DNA STRAND BREAKAGE; GENE TARGETING; HEAT TOLERANCE; HEATING; NONVIRAL GENE DELIVERY SYSTEM; NONVIRAL GENE THERAPY; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS;

EID: 33646199849     PISSN: 15309932     EISSN: 15309932     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Herweijer H, Wolff JA. Progress and prospects: naked DNA gene transfer and therapy. Gene Ther. 2003;10:453-458.
2. Sinn PL Jr, Sauter SL Jr, McCray PB Jr. Gene therapy progress and prospects: development of improved lentiviral and retroviral vectors - design, biosafety, and production. Gene Ther. 2005;12: 1089-1098.
3. Patil SD, Rhodes DG, Burgess DJ. DNA-based therapeutics and DNA delivery systems: a comprehensive review. AAPS J. 2005; 7:E61-E77.
4. Evans RK, Xu Z, Bohannon KE, Wang B, Bruner MW, Volkin DB. Evaluation of degradation pathways for plasmid DNA in pharmaceutical formulations via accelerated stability studies. J Pharm Sci. 2000;89: 76-87.
5. Potenza L, Cucchiarini L, Piatti E, Angelini U, Dacha M. Effects of high static magnetic field exposure on different DNAs. Bioelectromagnetics. 2004;25:352-355.
6. Li SH, Chow KC. Magnetic field exposure induces DNA degradation. Biochem Biophys Res Commun. 2001;280:1385-1388.
7. Kuo JH, Jan MS, Sung KC. Evaluation of the stability of polymer-based plasmid DNA delivery systems after ultrasound exposure. Int J Pharm. 2003;257:75-84.
8. Kuo JH, Lo YL, Shau MD, Cherng JY. A thermodynamic study of cationic polymer-plasmid DNA complexes by highly-sensitive differential scanning calorimetry. J Control Release. 2002;81:321-325.
9. Marguet E, Forterre P. Stability and manipulation of DNA at extreme temperatures. Methods Enzymol. 2001;334:205-215.
10. Walter E, Moelling K, Pavlovic J, Merkle HP. Microencapsulation of DNA using poly(DL-lactide-co-glycolide): stability issues and release characteristics. J Control Release. 1999;61:361-374.
11. Knight JD, Adami RC. Stabilization of DNA utilizing divalent cations and alcohol. Int J Pharm. 2003;264:15-24.
12. Banmeyer I, Marchand C, Clippe A, Knoops B. Human mitochondrial peroxiredoxin 5 protects from mitochondrial DNA damages induced by hydrogen peroxide. FEBS Lett. 2005;579:2327-2333.
13. Park S, You X, Imlay JA. Substantial DNA damage from submicromolar intracellular hydrogen peroxide detected in Hpx- mutants of Escherichia coli. Proc Natl Acad Sci USA. 2005;102:9317-9322.
14. Bakewell DJ, Ermolina I, Morgan H, Milner J, Feldman Y. Dielectric relaxation measurements of 12 kbp plasmid DNA. Biochim Biophys Acta. 2000;1493:151-158.
15. Baker-Javis J, Jones CA, Riddle B. Electrical Properties and Dielectric Relaxation of DNA in Solution. NIST Technical Note. Gaithersburg, MD: National Institute of Standards and Technology. 1998:1509.
16. Cole KS, Cole RH. Dispersion and absorption in dielectrics. J Chem Phys. 1941;9:341-351.
17. Gillay B, Funk DB. Changes in dielectric constant measurements due to settling and pressure.ASAE Annual Meeting; July 2003; Las Vegas, NV; 2003.
18. Buchner R, Barthel J. Dielectric relaxation in solutions. Annu Rep Prog Chem Sect C. 2001;97:349-382.