Increased FITC fluorescence on LPS stimulated neutrophils cultured in whole blood

Cell Biology International

Volumn 30, Issue 4, 2006, Pages 394-399

  Wilkins, R C ^a   Bellier, P V ^a   Kutzner, B C ^a   McNamee, J P ^a  

^a HEALTH CANADA   (Canada)

Author keywords

Apoptosis; Blood; Human; Neutrophil

Indexed keywords

ANTIBODY; CELL SURFACE MARKER; FLUORESCEIN ISOTHIOCYANATE; FLUOROCHROME; LIPOCORTIN 5; LIPOPOLYSACCHARIDE;

ADULT; APOPTOSIS; ARTICLE; BLOOD; BLOOD CULTURE; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; LYMPHOCYTE; NEUTROPHIL; PH;

ADULT; ANNEXIN A5; BLOOD; CARBOCYANINES; CELL CULTURE TECHNIQUES; CELLS, CULTURED; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENCE; HUMANS; LIPOPOLYSACCHARIDES; LYMPHOCYTES; NEUTROPHILS; PROPIDIUM;

EID: 33646183341     PISSN: 10656995     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cellbi.2005.12.006     Document Type: Article

References (12)

1. Babcock D.F. Examination of the intracellular ionic environment and of ionophore action by null point measurements employing the fluorescein chromophore. J Biol Chem 258 (1983) 6380-6389
2. Hampton M.B., Kettle A.J., and Winterbourn C.C. Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing. Blood 92 (1998) 3007-3017
3. Hertveldt K., Philippe J., Thierens H., Cornelissen M., Vral A., and De Ridder L. Flow cytometry as a quantitative and sensitive method to evaluate low dose radiation induced apoptosis in vitro in human peripheral blood lymphocytes. Int J Radiat Biol 71 (1997) 429-433
4. Homburg C.H., de Haas M., dem Borne A.E., Verhoeven A.J., Reutelingsperger C.P., and Roos D. Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro. Blood 85 (1995) 532-540
5. Kerr J.F., Wyllie A.H., and Currie A.R. Apoptosis: a basic biological phenomenon with wide range implications in tissues kinetics. Br J Cancer 26 (1972) 239-257
6. Martin S.J., Reutelingsperger C.P., McGahon A.J., Rader J.A., van Schie R.C., LaFace D.M., et al. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J Exp Med 182 (1995) 1545-1556
7. McNamee J.P., Bellier P.V., Kutzner B.C., and Wilkins R.C. Effect of pro-inflammatory cytokines on spontaneous apoptosis in leukocyte sub-sets within a whole blood culture. Cytokine 31 (2005) 161-167
8. Roos D., and Winterbourn C.C. Immunology: lethal weapons. Science 296 (2002) 669-671
9. Sabroe I., Prince L.R., Jones E.C., Horsburgh M.J., Foster S.J., Vogel S.N., et al. Selective roles for Toll-like receptor (TLR)2 and TLR4 in the regulation of neutrophil activation and life span. J Immunol 170 (2003) 5268-5275
10. Tait J.F., Smith C., and Wood B.L. Measurement of phosphatidylserine exposure in leukocytes and platelets by whole-blood flow cytometry with annexin V. Blood Cells Mol Dis 25 (1999) 271-278
11. Vermes I., Haanen C., Steffens-Nakken H., and Reutelingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 184 (1995) 39-51
12. Wilkins R.C., Kutzner B.C., Truong M., Sanchez-Dardon J., and McLean J.R.N. Analysis of radiation-induced apoptosis in human lymphocytes: flow cytometry using annexin V and propidium iodide versus the neutral comet assay. Cytometry 48 (2002) 14-19