Establishment and characterization of a novel genetic hybrid cell line for propagation of four pathogens

Journal of Virological Methods

Volumn 134, Issue 1-2, 2006, Pages 86-91

  Yan, Hua ^a   Li, Guocai ^a   Yan, Huishen ^b   Chen, Hongju ^a   Gong, Weijuan ^a   Qian, Li ^a   Ji, Mingchun ^a  

^a YANGZHOU UNIVERSITY   (China)

^b MEDICAL SCHOOL OF SOUTHEAST UNIVERSITY   (China)

Author keywords

Chromosomal DNA transfection; Genetic hybrid cell line; Pathogens

Indexed keywords

ANTIGEN; DNA; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M;

ARTICLE; BLOOD SAMPLING; CHROMOSOME NUMBER; CONTROLLED STUDY; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; CYTOPATHOGENIC EFFECT; DNA PURIFICATION; DNA TRANSFER; EMBRYO; GENETIC TRANSFECTION; HERPES SIMPLEX; HERPES SIMPLEX VIRUS; HUMAN; HUMAN CELL; HYBRID CELL; IMMUNOFLUORESCENCE; INFECTION SENSITIVITY; KARYOTYPING; MICROSCOPY; PREGNANCY; PRIORITY JOURNAL; RUBELLA; RUBELLA VIRUS; SUCROSE DENSITY GRADIENT CENTRIFUGATION; TOXOPLASMA; TOXOPLASMOSIS; VIRUS PURIFICATION;

ANIMALS; ANTIBODIES, PROTOZOAN; ANTIBODIES, VIRAL; CELL LINE; CHROMOSOMES; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA; EPIDERMIS; FEMALE; HERPES SIMPLEX; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; KARYOTYPING; LUNG; PREGNANCY; PREGNANCY COMPLICATIONS, INFECTIOUS; RUBELLA; RUBELLA VIRUS; SENSITIVITY AND SPECIFICITY; SIMPLEXVIRUS; TOXOPLASMA; TOXOPLASMOSIS; TRANSFECTION;

CYTOMEGALOVIRUS; HERPES; RUBELLA; RUBELLA VIRUS; SIMPLEXVIRUS; TOXOPLASMA;

EID: 33646142290     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2005.11.020     Document Type: Article

References (13)

1. Allan P.W., Parker W.B., Arnett G., Rose L.M., Shaddix S.C., Shewach D.S., Fourel I., and Secrist III J.A. Metabolism of the carbocyclic analog of 2′-deoxyguanosine (CdG) in human cells. Antivir. Res. 26 3 (1995) 266
2. Colbère-Garapin F., Duncan G., Pavio N., Pelletier I., and Petit I. An approach to understanding the mechanisms of poliovirus persistence in infected cells of neural or non-neural origin. Clin. Diagn. Virol. 9 2/3 (1998) 107-113
3. Grant B.C., Philip L.E., and Mark S.F. Effect of the R1 element on expression of the US3 and US6 immune evasion genes of human cytomegalovirus. Virology 288 1 (2001) 164-174
4. Kimura K., Ishioka K., Hashimoto K., Mori S., Suzutani T., Bowlin T.L., and Shigeta S. Isolation and characterization of NMSO3-resistant mutants of respiratory syncytial virus. Antivir. Res. 61 3 (2004) 165-171
5. Leemans I., Parviz H.R., and Arvid U. The indirect fluorescent antibody test based on schizont antigen for study of the sheep parasite Theileria lestoquardi. Vet. Parasitol. 69 1/2 (1997) 9-18
6. Maree S., and Janusz P.T. Preparation of recombinant African horse sickness virus VP7 antigen via a simple method and validation of a VP7-based indirect ELISA for the detection of group-specific IgG antibodies in horse sera. J. Virol. Meth. 125 1 (2005) 55-65
7. Nahmias A., Walls K., Stewart J., Flvnn W., and Herrmann K. The TORCH complex perinatal infections associated with toxoplasma, rubella virus, cytomegalovirus and herpes simplex virus. Paediatr. Res. 5 (1971) 405-406
8. Talbot P. Human cytomegalovirus: purification of enveloped virus and dense bodies. J. Gen. Virol. 36 (1977) 345-349
9. Timothy F.P., Galena R.V., Xavier A., Olga B., and Konstantin K.G. Overexpression of gK in gK-transformed cells collapses the Golgi apparatus into the endoplasmic reticulum inhibiting virion egress, glycoprotein transport, and virus-induced cell fusion. Virology 317 2 (2003) 237-252
10. Tsutsui Y., Sonta S., and Kashiwat A. Viral replication in Hela/fibroblast hybrid cells infected with human cytomegalovirus. Arch. Virol. 95 (1987) 29-40
11. Yan H., and Dai B. Preparation and application monoclonal antibody to HCMV. J. McAb 10 3 (1994) 27-30
12. Yan H., Shen H., Zhang Y., and Geng J. Study on HCMV replication in gene transferred cells. Acta Microbiol. Sin. OGI CA 38 6 (1998) 475-479
13. Yan H., Shan Q., and Li G.-C. Preparation and application of a capture ELISA kit for TORCH-IgM. J. Immunol. 20 2 (2004) 144-147