A cocktail of affinity-purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis

Diagnostic Microbiology and Infectious Disease

Volumn 55, Issue 1, 2006, Pages 65-68

  Harinath, Bhaskar Chinnaiah ^a   Kumar, Satish ^a   Roy, Santa Saha ^a   Hirudkar, Suvarna ^a   Upadhye, Vijay ^a   Shende, Niraj ^a  

^a MAHATMA GANDHI INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Cocktail antibody; ELISA; Immune complexed antigen; Mycobacterial ES antigen; Tuberculosis

Indexed keywords

ANTIBODY; ANTIGEN; MYCOBACTERIUM ANTIGEN;

ANTIGEN DETECTION; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BLOOD ANALYSIS; BLOOD SAMPLING; COMBINATORIAL CHEMISTRY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC APPROACH ROUTE; DISEASE MARKER; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNE COMPLEX DEPOSITION; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOREACTIVITY; MAJOR CLINICAL STUDY; MYCOBACTERIUM TUBERCULOSIS; PRIORITY JOURNAL;

ANTIBODIES, BACTERIAL; ANTIBODY SPECIFICITY; ANTIGEN-ANTIBODY COMPLEX; ANTIGENS, BACTERIAL; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MYCOBACTERIUM TUBERCULOSIS; SPUTUM; TUBERCULOSIS, PULMONARY;

EID: 33646125854     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2005.10.021     Document Type: Article

References (30)

1. Astekar M.D., Dhalla S., Mazerello T.B.M.S., and Samuel A.M. Study of M. tuberculosis antigen and antibody in cerebrospinal fluid and blood in tuberculous meningitis. Clin. Immunol. Immunopathol. 45 (1987) 29-34
2. Astekar M.D., and Samuel A.M. Circulating immune complexes in pulmonary tuberculosis. J. Clin. Lab. Immunol. 18 (1985) 91-95
3. Banerjee S., Gupta S., Kumar S., Shrikhande A.V., Reddy M.V.R., and Harinath B.C. Seroreactivity of 31 kDa and 41 kDa mycobacterial secretory proteins isolated from culture filtrate in extra pulmonary tuberculosis. Indian J. Pathol. Microbiol. 46 (2003) 261-264
4. 37Ra in vitro released antigen. Int. J. Tuberc. Lung Dis. 7 (2003) 278-283
5. Bothamley G.H., Rudd R., Festenstein F., and Ivanyi J. Clinical value of the measurement of Mycobacterium tuberculosis-specific antibody in pulmonary tuberculosis. Thorax 47 (1992) 270-275
6. Brostof J., Lenzini L., Rottali P., and Rottali L. Immune complexes in the spectrum of tuberculosis. Tubercle 62 (1981) 169-173
7. Chandramukhi A., Allen P.R.J., Keen M., and Ivanyi J. Detection of mycobacterial antigens and antibodies in the cerebrospinal fluid of patients with tuberculous meningitis. J. Med. Microbiol. 20 (1985) 239-247
8. 37Ra excretory-secretory ES-43 and ES-31 antigens at different stages of pulmonary tuberculosis. Biomed. Res. 15 (2004) 76-79
9. Gupta S., Shende N., Kumar S., and Harinath B.C. Detection of antibodies to a cocktail of mycobacterial excretory-secretory antigens by ELISA and immunoblotting. Curr. Sci. 88 (2005)
10. Houghton R.L., Lodes M.J., Dillon D.C., Reynolds L.D., Day C.H., McNeill P.D., Hendrickson R.C., Skeiky Y.A., Sampaio D.P., Badaro R., Lyashchenko K.P., and Reed S.G. Use of multiepitope polyproteins in serodiagnosis of active tuberculosis. Clin. Diagn. Lab. Immunol. Jul. 9 (2002) 883-891
11. Johnson N.M., McNicol M.W., Burtan-Kee E.J., and Mowbray J.F. Circulating immune complexes in tuberculosis. Thorax 36 (1981) 610-617
12. Kadival G.V., Mazarelo T.B.M.S., and Chaparas S.D. Sensitivity and specificity of enzyme-linked immunosorbent assay in detection of antigen in tuberculous meningitis. J. Med. Microbiol. 20 (1985) 901-904
13. Kadival G.V., Samuel A.M., Mazareto T.B., and Chaparas S.D. Radioimmunoassay for detection of Mycobacterium tuberculosis antigen in cerebrospinal fluids of patients with tuberculous meningitis. J. Infect. Dis. 155 (1987) 608-611
14. Kaliraj P., Ghirnikar S.N., and Harinath B.C. Fractionation and evaluation of W. bancrofti microfilarial antigens in immunodiagnosis of bancroftian filariasis. J. Expt. Biol. 20 (1982) 440-441
15. Krombovitis E., McMurry M.B., Lock P.E., Hendrickse W., and Holzel H. Rapid diagnosis of tuberculous meningitis by latex particle agglutination. Lancet 2 (1984) 1229-1231
16. Landowiski C.P., Godfrey H.P., Bentley-Hibbert S.I., Liu X., Huang Z., Sepulveda R., Huygen K., Gennaro M.L., Moy F.H., Lesley S.A., and Haak-Frendscho M. Combinatorial use of antibodies to secreted mycobacterial proteins in a host immune system-independent test for tuberculosis. J. Clin. Microbiol. (2001) 2418-2424
17. 37 Ra excretory secretory antigen in pulmonary tuberculosis. Indian. J. Biochem. Biophys. 33 (1996) 66-71
18. 37Ra. J. Biosci. 23 (1998) 19-23
19. Lyashchenko K.R., Colangele M., Houde H.A., Jahadali D., and Menizies Gennaro M.L. Heterogenous antibody responses in tuberculosis. Infect. Immune. 66 (1998) 3936-3940
20. May J.J., Katilus J., Henson P.M., and Dreisin R.B. The purification and identification of circulating immune complexes in tuberculosis. Am. Rev. Respir. Dis. 128 (1983) 922-925
21. 37Ra ESAS-7-an excretory-secretory antigen fraction of immunodiagnostic potential in pulmonary tuberculosis. Indian J. Clin. Biochem. 13 (1998) 98-105
22. 37Ra bacilli. Scand. J. Infect. Dis. 32 (2000) 551-556
23. Nair E.R., Banerjee S., Kumar S., Reddy M.V.R., and Harinath B.C. Purification and characterization of a 31kDa mycobacterial excretory-secretory antigenic protein with a diagnostic potential in pulmonary tuberculosis. Indian J. Ches. Dis. Allied. Sci. 43 (2001) 81-90
24. Nair E.R., Banerjee S., Kumar S., Reddy M.V.R., and Harinath B.C. Isolation of Mycobacterium tuberculosis 31kDa antigen protein of diagnostic interest from culture filtrate using anti-ES-31 antibody by affinity chromatography. Indian J. Clin. Biochem. 16 (2001) 132-135
25. Perkins M.D., Conde M.B., Martins M., and Kritski A.L. Serologic diagnosis of tuberculosis using simple commercial multiantigen assay. Chest 123 (2003) 107-112
26. Pramanik J., Lodam A.N., Reddy M.V.R., Narang P., and Harinath B.C. Increased yield of excretory secretory antigen with thyroxine supplement in in vitro culture of tubercle bacilli. Ind. J. Tub. 44 (1997) 185-190
27. Prasad G.B.K.S., Kharat I., and Harinath B.C. Detection of antimicrofilarial ES antigen-antibody in immune complexes in bancroftian filariasis by enzyme immunoassay. Trans. R. Soc. Trop. Med. Hyg. 77 (1983) 771-772
28. Radhakrishnan V.V., and Mathai A. Enzyme linked immunosorbent assay to detect M. tuberculosis antigen 5 and antimycobacterial antibody in cerebrospinal fluid of patients with tuberculous meningitis. J. Clin. Lab. Anal. 5 (1991) 233-237
29. Radhakrishnan V.V., Mathai A., and Sundaram P. Diagnostic significance of circulating immune complexes in patients with pulmonary tuberculosis. J. Med. Microbiol. 36 (1992) 128-131
30. Reddy M.V.R., Malhotra A., and Harinath B.C. Detection of circulating antigen in bancroiftian filariasis by sandwich ELISA using filaria serum IgG. Helminthology 58 (1984) 259-262