Efficient solubilization, activation, and purification of recombinant Cry45Aa of Bacillus thuringiensis expressed as inclusion bodies in Escherichia coli

Protein Expression and Purification

Volumn 47, Issue 1, 2006, Pages 144-151

  Okumura, Shiro ^a   Saitoh, Hiroyuki ^a   Wasano, Naoya ^a   Katayama, Hideki ^a   Higuchi, Kazuhiko ^a   Mizuki, Eiichi ^a   Inouye, Kuniyo ^b  

^a FUKUOKA INDUSTRIAL TECHNOLOGY CENTER   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

CACO 2 cells; Cry protein; Cytotoxic activity; Delta endotoxin; Parasporin

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; ENDOTOXIN; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; PARASPORIN; RECOMBINANT PROTEIN;

ARTICLE; CELL INCLUSION; CELL STRAIN CACO 2; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; SOLUBILITY;

BACTERIAL PROTEINS; BACTERIAL TOXINS; CACO-2 CELLS; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; ENDOTOXINS; ESCHERICHIA COLI; HEMOLYSIN PROTEINS; HUMANS; INCLUSION BODIES; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SOLUBILITY;

BACILLUS THURINGIENSIS; ESCHERICHIA COLI;

EID: 33646105851     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.10.011     Document Type: Article

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