Purification and characterization of a soluble form of the recombinant human galactose-β1,3-glucuronosyltransferase I expressed in the yeast Pichia pastoris

Protein Expression and Purification

Volumn 47, Issue 1, 2006, Pages 137-143

  Lattard, Virginie ^a   Fondeur Gelinotte, Magali ^a   Gulberti, Sandrine ^a   Jacquinet, Jean Claude ^b   Boudrant, Joseph ^c   Netter, Patrick ^a   Magdalou, Jacques ^a   Ouzzine, Mohamed ^a   Fournel Gigleux, Sylvie ^a  

^a UNIVERSITÉ DE LORRAINE   (France)

^b UNIVERSITÉ D'ORLÉANS   (France)

^c CNRS   (France)

Author keywords

Enzyme kinetic; Glucuronosyltransferase; Glycosaminoglycan; Substrate specificity

Indexed keywords

GALACTOSIDE; GLUCURONOSYLTRANSFERASE; GLUCURONYLTRANSFERASE GLCAT 1; GLUCURONYLTRANSFERASE GLCAT-1; RECOMBINANT PROTEIN; URIDINE DIPHOSPHATE GLUCURONIC ACID;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; PICHIA; SOLUBILITY;

CLONING, MOLECULAR; GALACTOSIDES; GLUCURONOSYLTRANSFERASE; HUMANS; KINETICS; PICHIA; RECOMBINANT PROTEINS; SOLUBILITY; SUBSTRATE SPECIFICITY; URIDINE DIPHOSPHATE GLUCURONIC ACID;

PICHIA PASTORIS;

EID: 33646092774     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.10.012     Document Type: Article

References (35)

1. Kjellen J., and Lindahl U. Proteoglycans: structures and interactions. Annu. Rev. Biochem. 60 (1991) 443-475
2. Lander A.D., and Selleck S.B. The elusive functions of proteoglycans: in vivo veritas. J. Cell Biol. 148 (2000) 227-232
3. Perrimon N., and Bernfield M. Specificities of heparan sulphate proteoglycans in developmental processes. Nature 404 (2000) 725-728
4. Schwartz N.B., and Domowicz M. Chondrodysplasias due to proteoglycan defects. Glycobiology 12 (2002) 57-68
5. Shriver Z., Liu D., and Sasisekharan R. Emerging views of heparan sulfate glycosaminoglycan structure/activity relationships modulating dynamic biological functions. Trends Cardiovasc. Med. 12 (2002) 71-77
6. van Horssen J., Wesseling P., van den Heuvel L.P., de Waal R.M., and Verbeek M.M. Heparan sulphate proteoglycans in Alzheimer's disease and amyloid-related disorders. Lancet Neurol. 2 (2003) 482-492
7. Wegrowski Y., and Maquart F.X. Involvement of stromal proteoglycans in tumour progression. Crit. Rev. Oncol. Hematol. 49 (2004) 259-268
8. Sugahara K., and Kitagawa H. Recent advances in the study of the biosynthesis and functions of sulfated glycosaminoglycans. Curr. Opin. Struct. Biol. 10 (2000) 518-527
9. Bai X., Wei G., Sinha A., and Esko J.D. Chinese hamster ovary cell mutants defective in glycosaminoglycan assembly and glucuronosyltransferase I. J. Biol. Chem. 274 (1999) 13017-13024
10. Kisilevsky R., and Szarek W.A. Novel glycosaminoglycan precursors as anti-amyloid agents part II. J. Mol. Neurosci. 19 (2002) 45-50
11. Chicaud P., Rademakers J.R., and Millet J. The beneficial effect of a β-d-xyloside, Iliparcil, in the prevention of postthrombolytic rethrombosis in the rat. Haemostasis 28 (1998) 313-320
12. Mani K., Havsmark B., Persson S., Kaneda Y., Yamamoto H., Sakurai K., Ashikari S., Habuchi H., Suzuki S., Kimata K., Malmstrom A., Westergren-Thorsson G., and Fransson L.A. Heparan/chondroitin/dermatan sulfate primer 2-(6-hydroxynaphthyl)-O-β-d-xylopyranoside preferentially inhibits growth of transformed cells. Cancer Res. 58 (1998) 1099-1104
13. Venkatesan N., Barré L., Benani A., Netter P., Magdalou J., Fournel-Gigleux S., and Ouzzine M. Stimulation of proteoglycan synthesis by glucuronosyltransferase-I gene delivery: a strategy to promote cartilage repair. Proc. Natl. Acad. Sci. USA 101 (2004) 18087-18092
14. Kitagawa H., Tone Y., Tamura J., Neumann K.W., Ogawa T., Oka O., Kawasaki T., and Sugahara K. Molecular cloning of glucuronosyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans. J. Biol. Chem. 273 (1998) 6615-6618
15. Pedersen L.C., Tsuchida K., Kitagawa H., Sugahara K., Darden T.A., and Negishi M. Heparan/chondroitin sulfate biosynthesis, structure,and mechanism of human glucuronyltransferase I. J. Biol. Chem. 275 (2000) 34580-34585
16. Boeggeman E.E., Ramakrishnan B., and Qasba P.K. The N-terminal stem region of bovine and human β1,4-galactosyltransferase I increases the in vitro folding efficiency of their catalytic domain from inclusion bodies. Protein Expr. Purif. 30 (2003) 219-229
17. Ouzzine M., Gulberti S., Netter P., Magdalou J., and Fournel-Gigleux S. Structure/function of the human Galβ1,3-glucuronosyltransferase I. Dimerization and functional activity are mediated by two crucial cysteine residues. J. Biol. Chem. 275 (2000) 28254-28260
18. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
19. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227 (1970) 680-685
20. Ouzzine M., Gulberti S., Levoin N., Netter P., Magdalou J., and Fournel-Gigleux S. The donor substrate specificity of the human β1,3-glucuronosyltransferase I toward UDP-glucuronic acid is determined by two crucial histidine and arginine residues. J. Biol. Chem. 277 (2002) 25439-25445
21. Gulberti S., Lattard V., Fondeur M., Jacquinet J.C., Mulliert G., Netter P., Magdalou J., Ouzzine M., and Fournel-Gigleux S. Phosphorylation and sulfation of oligosaccharide substrates critically influence the activity of human β1,4-galactosyltransferase 7 (GalT-I) and β1,3-glucuronosyltransferase I (GlcAT-I) involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans. J. Biol. Chem. 280 (2005) 1417-1425
22. Jacquinet J.C. An expeditious preparation of various sulfoforms of the disaccharide β-d-Galp-(1->3)-d-Galp, a partial structure of the linkage region of proteoglycans, as their 4-methoxyphenyl β-d-glycosides. Carbohydr. Res. 339 (2004) 349-359
23. Wang W.T., Le Donne N.C., Ackerman B., and Sweely C.C. Structural characterization of oligosaccharides by high-performance liquid chromatography, fast-atom bombardment-mass spectrometry, and exoglycosidase digestion. Anal. Biochem. 141 (1984) 366-381
24. 14C]glucuronic acid. Anal. Biochem. 109 (1980) 321-329
25. Fournel-Gigleux S., Jackson M.R., Wooster R., and Burchell B. Expression of a human liver cDNA encoding a UDP-glucuronosyltransferase catalysing the glucuronidation of hyodeoxycholic acid in cell culture. FEBS Lett. 243 (1989) 119-122
26. Segel I.H. Enzyme Kinetics (1975), John Wiley and Sons, New York
27. C. Breton, L. Snajdrova, C. Jeanneau, J. Koea, A. Imberty, Structures and mechanisms of glycosyltransferases superfamily, Glycobiology (2005) in press.
28. Gulberti S., Lattard V., Fondeur M., Jacquinet J.C., Mulliert G., Netter P., Magdalou J., Ouzzine M., and Fournel-Gigleux S. Modifications of the glycosaminoglycan-linkage region of proteoglycans: phosphorylation and sulfation determine the activity of the human β1,4-galactosyltransferase 7 and β1,3-glucuronosyltransferase I. Sci. World J. 5 (2005) 510-514
29. Bencurova M., Rendic D., Fabini G., Kopecky E.M., Altmann F., and Wilson I.B. Expression of eukaryotic glycosyltransferases in the yeast Pichia pastoris. Biochimie 85 (2003) 413-422
30. Sugumaran S., Katsman M., and Silbert J.E. Subcellular co-localization and potential interaction of glucuronosyltransferases with nascent proteochondroitin sulphate at Golgi sites of chondroitin synthesis. Biochem. J. 329 (1998) 203-208
31. Kakuda S., Oka S., and Kawasaki T. Purification and characterization of two recombinant human glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate in Escherichia coli. Protein Expr. Purif. 35 (2004) 111-119
32. Brandt A.E., Distler J., and Jourdian G.W. Biosynthesis of the chondroitin sulfate-protein linkage region: purification and properties of a glucuronosyltransferase from embryonic chick brain. Proc. Natl. Acad. Sci. USA 64 (1969) 374-380
33. Helting T., and Roden L. Biosynthesis of chondroitin sulfate. II. Glucuronosyl transfer in the formation of the carbohydrate-protein linkage region. J. Biol. Chem. 244 (1969) 2799-2805
34. Terayama K., Oka S., Sejki T., Miki Y., Nakamura A., Kozutsumi Y., Takio K., and Kawasaki T. Cloning and functional expression of a novel glucuronosyltransfersae involved in the biosynthesis of the carbohydrate epitope HNK1. Proc. Natl. Acad. Sci. USA 94 (1997) 6093-6098
35. Wei G., Bai X., Sarkar A.K., and Esko J.D. Formation of HNK-1 determinants and the glycosaminoglycan tetrasaccharide linkage region by UDP-GlcUA:galactoseβ1,3-glucuronosyltransferase. J. Biol. Chem. 274 (1999) 7857-7864