Effect of different hapten-carrier conjugation ratios and molecular orientations on antibody affinity against a peptide antigen

Journal of Immunological Methods

Volumn 311, Issue 1-2, 2006, Pages 198-206

  Pedersen, Maiken K ^a   Sorensen, Nanna S ^b   Heegaard, Peter M H ^b   Beyer, Natascha Helena ^a   Bruun, Leif ^a  

^a STATENS SERUM INSTITUT   (Denmark)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Affinity; Conjugation; Hapten; Immunisation; Peptide; Ratio

Indexed keywords

ANTIBODY; ANTIGEN; CARRIER PROTEIN; HAPTEN; OVALBUMIN; PEPTIDE; VEROTOXIN 2;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANTIBODY AFFINITY; ANTIBODY TITER; ARTICLE; CONJUGATE; CONJUGATION; ESCHERICHIA COLI; FEMALE; HAPTEN CARRIER COMPLEX; IMMUNIZATION; MASS SPECTROMETRY; MOUSE; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; ANTIBODY AFFINITY; CROSS-LINKING REAGENTS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; FEMALE; HAPTENS; IMMUNIZATION; IMMUNOCONJUGATES; MICE; MOLECULAR SEQUENCE DATA; OVALBUMIN; PEPTIDE FRAGMENTS; SHIGA-LIKE TOXIN II; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ANIMALIA; ESCHERICHIA COLI;

EID: 33646083005     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.02.008     Document Type: Article

References (21)

1. Bernatowicz M.S., and Matsueda G.R. Preparation of peptide-protein immunogens using N-succinimidyl bromoacetate as a heterobifunctional crosslinking reagent. Anal. Biochem. 155 (1986) 95-102
2. Friguet B., Chaffotte A.F., Djavadi-Ohaniance L., and Goldberg M.E. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J. Immunol. Methods 77 (1985) 305-319
3. Glynn L.E., and Steward M.W. Immunochemistry - an advanced textbook. 1st edition (1977), John Wiley and Sons Ltd
4. Griswold W.R. A quantitative relationship between antibody affinity and antibody avidity. Immunol. Invest. 16 (1987) 97-106
5. Hermanson G.T. Bioconjugate Techniques. 1st edition (1996), Academic Press
6. Huntington J.A., and Stein P.E. Structure and properties of ovalbumin. J. Chromatogr., B 756 (2001) 189-198
7. Imberechts, H., De Greve, H., Schlicker, C., Bouchet, H., Pohl, P., Charlier, G., Bertschinger, H., Wild, P., Vandekerckhove, J., Van Damme, J., 1992. Characterization of F107 fimbriae of Escherichia coli 107/86, which causes edema disease in pigs, and nucleotide sequence of the F107 major fimbrial subunit gene, fedA. Infect Immun. 60, 1963-1971.
8. Kussmann M., Nordhoff E., Rahbek-Nielsen H., Haebel S., Rossel-Larsen M., Jakobsen L., Globom J., Mirgorodskaya E., Kroll-Kristensen A., Palm L., and Roepstorff P. Matrix-assisted laser desorption/ionisation mass spectrometry sample preparation techniques designed for various peptide and protein analytes. J. Mass Spectrom. 32 (1997) 593-601
9. Loomans E.E.M.G., Roelen A.J.M., Van Damme H.S., Bloemers H.P.J., Gribnau T.C.J., and Schielen W.J.G. Assessment of the functional affinity constant of monoclonal antibodies using an improved enzyme-linked immunosorbent assay. J. Immunol. Methods 184 (1995) 207-217
10. Mitchison N.A. The carrier effect in the secondary immune response to hapten-protein conjugates: V. Use of antilymphocyte serum to deplete animals of helper cells. Eur. J. Immunol. 1 (1971) 68-75
11. Nimmo G.R., Lew A.M., Stanley C.M., and Steward M.W. Influence of antibody affinity on the performance of different antibody assays. J. Immunol. Methods 72 (1984) 177-187
12. Nencioni L., Mancini C., Pini C., and Doria G. T-cell-dependent oscillations of IgM antibody affinity during the immune response to DNP-Dextran to low or high epitope density. Immunology 47 (1982) 123-132
13. O'Connor T., Kane M.M., and Gosling JP. The dependence of the detection limit of reagent-limited immunoassay on antibody affinity. Biochem. Soc. Trans. 23 (1995) 393-400
14. Ovary Z., and Benacerraf B. Immunological specificity of the secondary response with dinitrophenylated proteins. Proc. Soc. Exp. Biol. Med. 114 (1963) 72-76
15. Parker D.C. T cell-dependent B cell activation. Ann. Rev. Immunol. 11 (1993) 331-340
16. Schots A., Van der Leede B.J., de Jongh E., and Egberts E. A method for the determination of antibody affinity using a direct ELISA. J. Immunol. Methods 109 (1988) 225-233
17. Schöneberg T., Erhard M.H., Kellner J., Kuhn S.O., and Losch U. The specificity and affinity of polyclonal antibodies raised in rabbits against a hapten conjugated to rabbit serum albumin. J. Immunoass. 16 (1995) 17-35
18. Suzuki N., Quesenberry M.S., Wang J.K., Lee R.T., Kobayashi K., and Lee Y.C. Efficient immobilization of proteins by modification of plate surface with polystyrene derivatives. Anal. Biochem. 247 (1997) 412-416
19. Wong S.S. Chemistry of protein conjugation and cross-linking. 1st edition (1993), CRC Press
20. Zinkernagel R.M. On natural and artificial vaccinations. Annu. Rev. Immunol. 21 (2003) 515-546
21. Zuckier L.S., Berkowitz E.Z., Sattenberg R.J., Zhao Q.H., Deng H.F., and Scharff M.D. Influence of affinity and antigen density on antibody localization in a modifiable tumor targeting model. Cancer Res. 60 (2000) 7008-7013