Protein instability during HIC: Describing the effects of mobile phase conditions on instability and chromatographic retention

Biotechnology and Bioengineering

Volumn 93, Issue 6, 2006, Pages 1177-1189

  Xiao, Yunzhi ^a   Freed, Alexander S ^a   Jones, Tara Tibbs ^a   Makrodimitris, Kostantinos ^a,b   O'Connell, John P ^a   Fernandez, Erik J ^a  

^a University of Virginia   (United States)

^b Johns Hopkins University   (United States)

Author keywords

Alpha lactalbumin; Hydrogen deuterium isotope exchange; Hydrophobic interaction chromatography; Protein adsorption; Protein purification; Protein stability

Indexed keywords

ADSORPTION; AMMONIUM COMPOUNDS; HYDROPHOBIC CHROMATOGRAPHY; PHASE TRANSITIONS; SALTS;

ALPHA LACTALBUMIN; HYDROGEN-DEUTERIUM ISOTOPE EXCHANGE; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; PROTEIN ADSORPTION; PROTEIN PURIFICATION; PROTEIN STABILITY;

PROTEINS;

ALPHA LACTALBUMIN;

ARTICLE; CHROMATOGRAPHY; MASS SPECTROMETRY; MATHEMATICAL ANALYSIS; PROTEIN DENATURATION; PROTEIN STABILITY; THERMODYNAMICS;

ADSORPTION; ALGORITHMS; AMMONIUM SULFATE; ANIMALS; CALCIUM; CATTLE; CHROMATOGRAPHY, GEL; COMPUTER SIMULATION; DEUTERIUM OXIDE; EDETIC ACID; HYDROPHOBICITY; LACTALBUMIN; MASS SPECTROMETRY; MODELS, THEORETICAL; PROTEIN DENATURATION; PROTEINS; SEPHAROSE; TEMPERATURE; THERMODYNAMICS; WATER;

EID: 33646052010     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20826     Document Type: Article

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