Measurement and analysis of intracellular ATP levels in metabolically engineered Zymomonas mobilis fermenting glucose and xylose mixtures

Biotechnology Progress

Volumn 22, Issue 2, 2006, Pages 359-368

  Sáez Miranda, Juan Carlos ^a   Saliceti Piazza, Lorenzo ^a   McMillan, James D ^b  

^a UNIVERSITY OF PUERTO RICO   (Puerto Rico)

^b NATIONAL RENEWABLE ENERGY LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETIC ACID; FERMENTATION; GLUCOSE; METABOLISM; PH EFFECTS; SUGAR (SUCROSE);

BATCH FERMENTATIONS; INHIBITORY EFFECT; INTRACELLULAR ATP ACCUMULATION; ZYMOMONAS MOBILIS;

ADENOSINETRIPHOSPHATE;

ADENOSINE TRIPHOSPHATE; GLUCOSE; XYLOSE;

ARTICLE; CLASSIFICATION; FERMENTATION; GENETICS; KINETICS; METABOLISM; MUTATION; ZYMOMONAS;

ADENOSINE TRIPHOSPHATE; FERMENTATION; GLUCOSE; KINETICS; MUTATION; XYLOSE; ZYMOMONAS;

ZYMOMONAS MOBILIS;

EID: 33646024970     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp050115c     Document Type: Article

References (42)

1. Joachimsthal, E. L.; Haggett, K. D.; Rogers, P. L. Evaluation of recombinant strains of Zymomonas mobilis for ethanol production from glucose/xylose media. Appl. Biochem. Biotechnol. 1999, 77-79, 147-157.
2. Joachimsthal, E. L.; Rogers, P. L. Characterization of a high-productivity recombinant strain of Zymomonas mobilis for ethanol production from glucose/xylose mixtures. Appl. Biochem. Biotechnol. 2000, 84-86, 343-356.
3. Lawford, H. G.; Rousseau, J. D. Performance testing of Zymomonas mobilis metabolically engineered for cofermentation of glucose, xylose, and arabinose. Appl. Biochem. Biotechnol. 2002, 98-100, 429-448.
4. Mohagheghi, A.; Evans, K.; Chou, Y.; Zhang, M. Cofermentation of glucose, xylose, and arabinose by genomic DNA-integrated xylose/arabinose fermenting strain of Zymomonas mobilis AX101. Appl. Biochem. Biotechnol. 2002, 98-100, 277-293.
5. Mohagheghi, A.; Dowe, N.; Schell, D.; Chou, Y.; Eddy, C.; Zhang, M. Performance of a newly developed integrant of Zymomonas mobilis for ethanol production on corn stover hydrolysate. Biotechnol. Lett. 2004, 26, 321-325.
6. McMillan, J. D. Bioethanol production: status and prospects. Renewable Energy 1997, 10 (2/3), 295-302.
7. Gong, C. S.; Cao, N. J.; Du, J.; Tsao, G. T. Ethanol production from renewable resources. Adv. Biochem. Eng./Biotechnol. 1999, 65, 207-241.
8. Knauf, M.; Moniruzzaman, M. Lignocellulosic biomass processing: a perspective. Int. Sugar J. 2004, 106 (1263), 147-150.
9. Jeon, Y. J.; Svenson, C. J.; Joachimsthal, E. L.; Rogers, P. L. Kinetic analysis of ethanol production by an acetate-resistant strain of recombinant Zymomonas mobilis. Biotechnol. Lett. 2002, 24, 819-824.
10. Joachimsthal, E. L.; Haggett, K. D.; Jang, J.; Rogers, P. L. A mutant of Zymomonas mobilis ZM4 capable of ethanol production from glucose in the presence of high acetate concentrations. Biotechnol. Lett. 1998, 20 (2), 137-142.
11. Lawford, H. G.; Rousseau, J. D. The effect of acetic acid on fuel ethanol production by Zymomonas. Appl. Biochem. Biotechnol. 1993, 39/40, 687-699.
12. Thomas, K. C.; Hynes, S. H.; Ingledew, W. M. Influence of medium buffering capacity on inhibition of Saccharomyces cerevisiae growth by acetic and lactic acids. Appl. Environ. Microbiol. 2002, 68 (4), 1616-1623.
13. Lawford, H. G.; Rousseau, J. D. The pH-dependent energetic uncoupling of Zymomonas by acetic acid. Appl. Biochem. Biotechnol. 1994, 45/46, 437-448.
14. Kim, I. S.; Barrow, K. D.; Rogers, P. L. Nuclear magnetic resonance studies of acetic acid inhibition of rec Zymomonas mobilis ZM4(pZB5). Appl. Biochem. Biotechnol. 2000, 84-86, 357-370.
15. 31P Nuclear magnetic resonance studies of ethanol inhibition in Zymomonas mobilis. Arch. Microbiol. 1993, 159, 484-490.
16. Lawford, H. G.; Ruggiero, A. Production of fuel alcohol by Zymomonas: effect of pH on maintenance and growth-associated metabolism. Biotechnol. Appl. Biochem. 1990, 12, 206-211.
17. Zhang, M.; Eddy, C.; Deanda, K.; Finkelstein, M.; Picataggio, S. Metabolic engineering of a pentose metabolism pathway in ethanologenic Zymomonas mobilis. Science 1995, 267, 240-243.
18. Deanda, K.; Zhang, M.; Eddy, C.; Picataggio, S. Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering. Appl. Environ. Microbiol. 1996, 62, 4465-4470.
19. Stanley, P. E. A review of bioluminescent ATP techniques in rapid microbiology. J. Biolumin. Chemilumin. 1989, 4, 375-380.
20. Chu, C. P.; Lee, D. J.; Chang, B.-V.; Liao, C. S. Using ATP bioluminescence technique for monitoring microbial activity in sludge. Biotechnol. Bioeng. 2001, 75, 469-474.
21. Kim, I. S.; Barrow, K. D.; Rogers, P. L. Kinetic and nuclear magnetic resonance studies of xylose metabolism by recombinant Zymomonas mobilis ZM4(pZB5). Appl. Environ. Microbiol. 2000, 66 (1), 186-193.
22. 31P Nuclear magnetic resonance studies of the fermentation of glucose to ethanol by Zymomonas mobilis. J. Biol. Chem. 1984, 259(9), 5711-5716.
23. Theobald, U.; Mailinger, W.; Reuss, M.; Rizzi, M. In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique. Anal. Biochem. 1993, 214, 31-37.
24. Bhattacharya, M.; Fuhrman, L.; Ingram, A.; Nickerson, K. W.; Conway, T. Single-run separation and detection of multiple metabolic intermediates by anion-exchange high-performance liquid chromatography and application to cell pool extracts prepared from Escherichia coli. Anal. Biochem. 1995, 232, 98-106.
25. Stocchi, V.; Cucchiarini, L.; Magnani, M.; Chiarantini, L.; Palma, P.; Crescentini, G. Simultaneous extraction and reverse-phase high-performance liquid chromatographic determination of adenine and pyridine nucleotides in human red blood cells. Anal. Biochem. 1985, 746, 118-124.
26. Tian, W.; Braunstein, L. D.; Pang, J.; Stuhlmeier, K. M.; Xi, Q.; Tian, X.; Stanton, R. C. Importance of glucose-6-phosphate dehydrogenase activity for cell growth. J. Biol. Chem. 1998, 273 (17), 10609-10617.
27. Grob, M. K.; O'Brien, K.; Chu, J. J.; Chen, D. D. Y. Optimization of cellular nucleotide extraction and sample preparation for nucleotide pool analyses using capillary electrophoresis. J. Chromatogr. B 2003, 788, 103-111.
28. Cole, H. A.; Wimpenny, J. W. T.; Hughes, D. E. The ATP pool in Escherichia coli. I. Measurement of the pool using a modified luciferase assay. Biochim. Biophys. Acta 1967, 143, 445-453.
29. Lazdunski, A.; Belaich, J. P. Uncoupling in bacterial growth: ATP pool variation in Zymomonas mobilis cells in relation to different uncoupling conditions of growth. J. Gen. Microbiol. 1972, 70, 187-197.
30. Schram, E.; Weyens-Van Witzenburg, A. Improved ATP methodology for biomass assays. J. Biolumin. Chemilumin. 1989, 4, 390-398.
31. Funabashi, H.; Imajo, T.; Kojima, J.; Kobatake, E.; Aizawa, M. Bioluminescent monitoring of intracellular ATP during fermentation. Luminescence 1999, 14, 291-296.
32. Gracia, E.; Fernández, A.; Conchello, P.; Alabart, J. L.; Pérez, M.; Amorena, B. In vitro development of Staphylococcus aureus biofilms using slime-producing variants and ATP-bioluminescence for automated bacterial quantification. Luminescence 1999, 14, 23-31.
33. Lundin, A. Use of firefly luciferase in ATP-related assays of biomass, enzymes, and metabolites. Methods Enzymol. 2000, 305, 346-370.
34. Dhople, A. M.; Hanks, J. H. Quantitative extraction of adenosine triphosphate from cultivable and host-grown microbes: calculation of adenosine triphosphate pools. Appl. Microbiol. 1973, 26 (3), 399-403.
35. Ishida, A.; Yoshikawa, T.; Nakazawa, T.; Kamidate, T. Enhanced firefly bioluminescence assay of ATP in the presence of ATP extradants by using diethylaminoethyl-dextran. Anal. Biochem. 2002, 305, 236-241.
36. Lasko, D. R.; Wang, D. I. C. On-line monitoring of intracellular ATP concentration in Escherichia coli fermentations. Biotechnol. Bioeng. 1996, 52, 364-372.
37. Gikas, P.; Livingston, A. G. Use of ATP to characterize biomass viability in freely suspended and immobilized cell bioreactors. Biotechnol. Bioeng. 1993, 42, 1337-1351.
38. Forrest, W. W. Adenosine triphosphate pool during the growth cycle in Streptococcus faecalis. J. Bacteriol. 1965, 90 (4), 1013-1018.
39. Toh, H.; Doelle, H. Changes in the growth and enzyme level of Zymomonas mobilis under oxygen-limited conditions at low glucose concentration. Arch. Microbiol. 1997, 168, 46-52.
40. Turner Biosystems Applications Note: A TD-20/20 Luminometer method for ATP Measurements, http://www.turnerdesigns.com/t2/doc/appnotes/998_2621.html. Active June 2005.
41. Sáez-Miranda, J. C.; Saliceti-Piazza, L.; McMillan, J. D. Kinetic and ATP maintenance studies of a metabolically engineered Zymomonas mobilis fermenting glucose and xylose mixtures. American Institute of Chemical Engineers (AIChE) Annual Meeting Conference Proceedings, Austin, Texas, Nov. 7-12, Paper 29a; AIChE: New York, 2004.
42. Lawford, H. G.; Rousseau, J. D.; Mohagheghi, A.; McMillan, J. D. Continuous fermentation studies with xylose-utilizing recombinant Zymomonas mobilis. Appl Biochem. Biotechnol. 2000, 84-86, 295-310.