Overdiagnosis of Entamoeba histolytica and Entamoeba dispar in Nicaragua: A Microscopic, Triage Parasite Panel and PCR Study

Archives of Medical Research

Volumn 37, Issue 4, 2006, Pages 529-534

  Leiva, Byron ^a,b,c   Lebbad, Marianne ^a,b   Winiecka Krusnell, Jadwiga ^b   Altamirano, Isabel ^c   Tellez, Aleyda ^a,b,c   Linder, Ewert ^a,b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b SWEDISH INSTITUTE FOR INFECTIOUS DISEASE CONTROL   (Sweden)

^c Universidad Nacional Autonoma de Nicaragua   (Nicaragua)

Author keywords

Diagnostic problems; Diarrhea; E. dispar; E. histolytica; PCR

Indexed keywords

ADOLESCENT; AMEBIASIS; ARTICLE; COCCIDIA; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; DIAGNOSTIC VALUE; DIARRHEA; DIENTAMOEBA; EMERGENCY HEALTH SERVICE; ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; EVALUATION; FECES ANALYSIS; FEMALE; HEALTH CENTER; HUMAN; INTESTINE PARASITE; MAJOR CLINICAL STUDY; MALE; MICROSCOPY; NICARAGUA; PARASITE IDENTIFICATION; PARASITE PREVALENCE; POLYMERASE CHAIN REACTION; STAINING;

ANIMALS; DIAGNOSTIC ERRORS; ENTAMOEBA; ENTAMOEBIASIS; HUMANS; MICROSCOPY; NICARAGUA; POLYMERASE CHAIN REACTION; TRIAGE;

EID: 33646020371     PISSN: 01884409     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.arcmed.2005.10.009     Document Type: Article

References (28)

1. Ravdin J.I. Amebiasis. Clin Infect Dis 20 (1995) 1453-1464
2. Walsh J.A. Problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality. Rev Infect Dis 8 (1986) 228-238
3. Diamond L.S., and Clark C.G. A redescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925. J Eukaryot Microbiol 40 (1993) 340-344
4. WHO. Amebiasis. Wkly Epidemiol Rec (1997) 97-98
5. Jackson T. Epidemiology. In: Ravdin J. (Ed). Amebiasis (2000), Imperial College Press, London 47-63
6. García L., and Bruckner D. Macroscopic and microscopic examination of fecal specimens. In: Garcia L.S. (Ed). Diagnostic Medical Parasitology (1997), ASM Press, Washington, DC 608-651
7. Gatti S., Swierczynski G., Robinson F., Anselmi M., Corrales J., Moreira J., et al. Amebic infections due to the Entamoeba histolytica-Entamoeba dispar complex: a study of the incidence in a remote rural area of Ecuador. Am J Trop Med Hyg 67 (2002) 123-127
8. Kebede A., Verweij J.J., Petros B., and Polderman A.M. Short communication: misleading microscopy in amebiasis. Trop Med Int Health 9 (2004) 651-652
9. Kebede A., Verweij J.J., Endeshaw T., Messele T., Tasew G., Petros B., et al. The use of real-time PCR to identify Entamoeba histolytica and E. dispar infections in prisoners and primary-school children in Ethiopia. Ann Trop Med Parasitol 98 (2004) 43-48
10. Pinheiro S.M., Carneiro R.M., Aca I.S., Irmao J.I., Morais Jr. M.A., Coimbra M.R., et al. Determination of the prevalence of Entamoeba histolytica and E. dispar in the pernambuco state of northeastern Brazil by a polymerase chain reaction. Am J Trop Med Hyg 70 (2004) 221-224
11. Lawson L.L., Bailey J.W., Beeching N.J., Gurgel R.G., and Cuevas L.E. The stool examination reports amoeba cysts: should you treat in the face of over diagnosis and lack of specificity of light microscopy?. Trop Doct 34 (2004) 28-30
12. Lopez Cruz S., Salazar L., Urroz L., and Anderson M. Diagnostic certainty in intestinal amebiasis in Managua. Rev Cubana Med Trop 43 (1991) 80-84
13. Tellez A., Morales W., Rivera T., Meyer E., Leiva B., and Linder E. Prevalence of intestinal parasites in the human population of Leon, Nicaragua. Acta Trop 66 (1997) 119-125
14. Young K.H., Bullock S.L., Melvin D.M., and Spruill C.L. Ethyl acetate as a substitute for diethyl ether in the formalin-ether sedimentation technique. J Clin Microbiol 10 (1979) 852-853
15. Clark C.G., and Diamond L.S. Entamoeba histolytica: a method for isolate identification. Exp Parasitol 77 (1993) 450-455
16. Kebede A., Verweij J., Dorigo-Zetsma W., Sanders E., Messele T., van Lieshout L., et al. Over-diagnosis of amebiasis in the absence of Entamoeba histolytica among patients presenting with diarrhoea in Wonji and Akaki, Ethiopia. Trans R Soc Trop Med Hyg 97 (2003) 305-307
17. WHO/PAHO/UNESCO. Report. A consultation with experts on amebiasis. Mexico City, Mexico. Epidemiol Bull 1997;13-14.
18. Blessmann J., Van Linh P., Nu P.A., Thi H.D., Muller-Myhsok B., Buss H., et al. Epidemiology of amebiasis in a region of high incidence of amebic liver abscess in central Vietnam. Am J Trop Med Hyg 66 (2002) 578-583
19. Sanchez-Guillen M del C., Perez-Fuentes R., Salgado-Rosas H., Ruiz-Arguelles A., Ackers J., Shire A., et al. Differentiation of Entamoeba histolytica/Entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amebiasis. Am J Trop Med Hyg 66 (2002) 731-737
20. Tachibana H., Kobayashi S., Takehoshi M., and Ihara S. Distinguishing pathogenic isolates of Entamoeba histolytica by polymerase chain reaction. J Infect Dis 164 (1991) 825-826
21. Heckendorn F., N'Goran E.K., Felger I., Vounatsou P., Yapi A., Oettli A., Marti H.P., et al. Species-specific field testing of Entamoeba spp. in an area of high endemicity. Trans R Soc Trop Med Hyg 96 (2002) 521-528
22. Nesbitt R.A., Mosha F.W., Katki H.A., Ashraf M., Assenga C., and Lee C.M. Amebiasis and comparison of microscopy to ELISA technique in detection of Entamoeba histolytica and Entamoeba dispar. J Natl Med Assoc 96 (2004) 671-677
23. Mank T.G., Zaat J.O., Blotkamp J., and Polderman A.M. Comparison of fresh versus sodium acetate acetic acid formalin preserved stool specimens for diagnosis of intestinal protozoal infections. Eur J Clin Microbiol Infect Dis 14 (1995) 1076-1081
24. Chacin-Bonilla L., Estevez J., Monsalve F., and Quijada L. Cyclospora cayetanensis infections among diarrheal patients from Venezuela. Am J Trop Med Hyg 65 (2001) 351-354
25. Ribes J.A., Seabolt J.P., and Overman S.B. Point prevalence of Cryptosporidium, Cyclospora, and Isospora infections in patients being evaluated for diarrhea. Am J Clin Pathol 122 (2004) 28-32
26. Ortega Y.R., Sterling C.R., and Gilman R.H. Cyclospora cayetanensis. Adv Parasitol 40 (1998) 399-418
27. Sharp S.E., Suarez C.A., Duran Y., and Poppiti R.J. Evaluation of the Triage Micro Parasite Panel for detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum in patient stool specimens. J Clin Microbiol 39 (2001) 332-334
28. Garcia L.S., Shimizu R.Y., and Bernard C.N. Detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum antigens in human fecal specimens using the triage parasite panel enzyme immunoassay. J Clin Microbiol 38 (2000) 3337-3340