Extraction of the outer membrane proteins of H. pylori and evaluation of their presence in stool of the infected individuals

Iranian Biomedical Journal

Volumn 8, Issue 2, 2004, Pages 83-88

  Sheikhian, Ali ^a   Hassan, Zuhair M ^a   Mustafaie, Ali ^b   Shokri, Fazel ^c   Malekzadeh, Reza ^d   Siavoshi, Farideh ^e  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^c TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^d TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^e UNIVERSITY OF TEHRAN   (Iran)

Author keywords

Affinity chromatography; H. pylori; Outer membrane proteins (OMP); SDS PAGE; Stool

Indexed keywords

ANTIGEN; ANTISERUM; BACTERIAL ANTIGEN; OUTER MEMBRANE PROTEIN;

ADULT; AFFINITY CHROMATOGRAPHY; ANTIGEN DETECTION; ARTICLE; BACTERIAL INFECTION; BACTERIUM ISOLATION; CLINICAL ARTICLE; CONCENTRATION (PARAMETERS); EVALUATION; EXTRACTION; FECES ANALYSIS; FEMALE; HELICOBACTER PYLORI; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; MALE; MOLECULAR WEIGHT;

BACTERIA (MICROORGANISMS); HELICOBACTER PYLORI; ORYCTOLAGUS CUNICULUS;

EID: 33645983938     PISSN: 1028852X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Torres, J., Perez-Perez, G., Goodman, K.J., Atherton, J.C., Gold, B.D., Harris, P.R., Garza, A.M.L., Guarner, J. and Munoz, O. (2000) A comprehensive review of the natural history of Helicobacter Pylori infection in children. Arch. Med. Res. 31: 431-469.
2. Doig, P., Exner, M.M., Hancock, R.E.W. and Trust, T.J. (1995) Isolation and characterization of a conserved porin protein from Helicobacter pylori. J. Bacteriol. 177: 5447-5452.
3. Faigel, D.O., Magaret, N., Corless, C., Lieberman, D.A. and Fennerty, M.B. (2000) Evaluation of rapid antibody tests for the diagnosis of Helicobacter pylori infection. Am. J. Gastroenterol. 95: 72-77.
4. Weijnen, C.F., Hendriks, H.A., Hoes, A.W., Verweij, W.M., Verheij, T.J. and De Wit, N.J. (2001) New immunoassay for the detection of Helicobacter pylori infection compared with urease test, 13c breath test and histology: validation in the primary care setting. J. Microbiol. Methods 46: 235-240.
5. Vakil, N., Rhew, D., Soll, A. and Ofman, J.J. (2000) The cost-effectiveness of diagnostic testing strategies for Helicobacter pylori. Am. J. Gastroenterol. 95: 1691-1698.
6. Vaira, D. and Vakil, N. (2001) Blood, urine, stool, breath, money, and Helicobacter pylori. Gut 48: 287-289.
7. Nishizono, A., Gotoh, T., Fujioka, T., Murakami, K., Kubota, T., Nasu, M., Watanabe, M., and Mifune, K. (1998) Serological assessment of the early response to eradication therapy using an immunodominant outer membrane protein of Helicobacter pylori. Clin. Diagn. Lab. Immunol. 5: 856-861.
8. Suzuki, N., Wakasugi, M., Nakaya, S., Kokubo, N., Sato, M., Kajiyama, H., Takahashi, R., Hirata, H., Ezure, Y., Fukuda, Y. and Shimoyama, T. (2002) Production and application of new monoclonal antibodies specific for a fecal Helicobacter pylori antigen. Clin. Diagn. Lab. Immuno. 9: 75-78.
9. Yongliang, Z., Jie, L., Da, L., Qin, D., Keda, Q. and Quindong, W. (2002) H. Pylori antigen and its igg, iga-type specific immunocomplexes in sera from patients with Helicobacter Pylori infection. Chin. Med. J. 115: 381-383.
10. Suzuki, N., Wakasugi, M., Nakaya, S., Kokubo, N., Sato, M., Kajiyama, H., Takahashi, R., Hirata, H., Ezure, Y., Fukuda, Y. and Shimoyama, T. (2002) Catalase, a specific antigen in the feces of human subjects infected with Helicobacter pylori. Clin. Diagn. Lab. Immunol. 9: 784-788.
11. Ge, Z., Doig, P. and Fox, J.G. (2001) Characterization of proteins in the outer membrane preparation of a murine pathogen, Helicobacter bilis. Infect. Immun. 69: 3502-3506.
12. Crabtree, J.E. (1996) Immune and inflammatory responses to Helicobacter pylori infection. Scand. J. Gastroenterol. Suppl. 215: 3-10.
13. Rosenbusch, J.P. (1974) Characterization of the major envelope proteins from Eschericia coli. J. Biol. Chem. 249: 8019-8029.
14. Schnaitman, C.A. (1970) Examination of the protein composition of the cell envelope of Eschericia coli by polyacrylamide gel electerophoresis. J. Bacteriol. 104: 882-889.
15. Johnstone, A. and Thorpe, R. (1996) "Immunochemistry in Practice", third edition, Blackwell Science Ltd., London.
16. Henslee, J.G., Vachula, E.D., Paxton, R.J., Matias, M.S., Shively, J.E., Rittenhouse, H.J. and Tomita, J.T. (1992) Identification and chracterization of new antigenic fragments related to carcinoembryonic antigen in adult feces. Cancer Res. 52: 4175-4182.
17. Moss, D.M., Mathews, H.M., Visvesara, G.S., Dickerson, J.W. and Walker, E.M. (1991) Purification and characterization of giardia lamblia antigens in the feces of mongolian Gerbils. J. Clin. Microbiol. 29: 21-26.
18. Debrow, R. and Yannielli, L. (1992) Fecal protein marker of colorectal cancer. Am. J. Gastrientero. 87: 854-858.
19. Maass, N., Delgado, E. and Knobloch, J. (1992) Isolation of an immunodiagnostic taenia solium coproantigen. Trop. Med. Parasitol. 43: 201-202.
20. Laemmli, U.K. (1970) Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
21. Towbin, H., Staehelin, T. and Gordon, J. (1970) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76: 4350-4354.
22. Zhongming, G.E., Doig, P. and Fox, J.G. (2001) Characterization of proteins in the outer membrane preparation of a murine pathogen, Helicobacter bilis. Infect. Immun. 69: 3502-3506.