Half-of-sites binding of orotidine 5′-phosphate and α-D-5-phosphorylribose 1-diphosphate to orotate phosphoribosyltransferase from Saccharomyces cerevisiae supports a novel variant of the theorell-chance mechanism with alternating site catalysis

Biochemistry

Volumn 45, Issue 16, 2006, Pages 5330-5342

  McClard, Ronald W ^a   Holets, Edward A ^a   MacKinnon, Andrew L ^a,b   Witte, John F ^a  

^a REED COLLEGE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CHROMATOGRAPHY; ENTROPY; ENZYME KINETICS; ENZYMES; MAGNETIZATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PHOSPHATES;

APOENZYMES; BINDING STOICHIOMETRY; GEL-FILTRATION CHROMATOGRAPHY; SACCHAROMYCES CEREVISIAE;

BIOCHEMISTRY;

ALPHA DEXTRO 5 PHOSPHORYLRIBOSE 1 DIPHOSPHATE; MAGNESIUM ION; OROTATE PHOSPHORIBOSYLTRANSFERASE; OROTIC ACID; OROTIDINE; OROTIDINE 5' PHOSPHATE; RIBOSE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHEMICAL REACTION KINETICS; COMPLEX FORMATION; DIALYSIS; ENTROPY; ENZYME KINETICS; ENZYME SUBUNIT; GEL FILTRATION CHROMATOGRAPHY; ISOTHERMAL TITRATION CALORIMETRY; NONHUMAN; PHOSPHORUS NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; STEADY STATE; STOICHIOMETRY; ULTRAVIOLET SPECTROSCOPY;

BINDING SITES; CALORIMETRY; CATALYSIS; CHROMATOGRAPHY, GEL; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, CHEMICAL; MOLECULAR STRUCTURE; OROTATE PHOSPHORIBOSYLTRANSFERASE; PHOSPHORIBOSYL PYROPHOSPHATE; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY; TITRIMETRY; URIDINE MONOPHOSPHATE;

SACCHAROMYCES CEREVISIAE;

EID: 33645963745     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051650o     Document Type: Article

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