Quantitative assessment of arginine methylation in free versus protein-incorporated amino acids in vitro and in vivo using protein hydrolysis and high-performance liquid chromatography

BioTechniques

Volumn 40, Issue 3, 2006, Pages 305-310

  Bulau, Patrick ^a,b   Zakrzewicz, Dariusz ^a   Kitowska, Kamila ^a   Wardega, Birgit ^a   Kreuder, Joachim ^a   Eickelberg, Oliver ^a  

^a JUSTUS LIEBIG UNIVERSITY GIESSEN   (Germany)

^b JUSTUS LIEBIG UNIVERSITY GIESSEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ASYMMETRIC DIMETHYLARGININE (ADMA); PROTEIN ARGININE METHYLTRANSFERASES (PRMT); QUANTITATIVE ASSESSMENTS; SYMMETRIC DIMETHYLARGININE (SDMA);

CHEMICAL MODIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; METHYLATION; PROTEINS; TISSUE;

AMINO ACIDS;

AMINO ACID DERIVATIVE; ARGININE; N(G) METHYLARGININE; N(G),N(G) DIMETHYLARGININE; PROTEASOME; PROTEIN ARGININE METHYLTRANSFERASE; PROTEOME; S ADENOSYLMETHIONINE; SYMMETRIC DIMETHYLARGININE; UNCLASSIFIED DRUG; AMINO ACID; PROTEIN;

ACCURACY; ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL LYSATE; CELL PROLIFERATION; ENZYME INHIBITION; ENZYME MECHANISM; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NONHUMAN; PRECIPITATION; PROTEIN HYDROLYSIS; PROTEIN METHYLATION; PROTEIN PROCESSING; QUANTITATIVE ANALYSIS; REGULATORY MECHANISM; RNA TRANSPORT; SEPARATION TECHNIQUE; SIGNAL TRANSDUCTION; CHEMISTRY; HYDROLYSIS; LUNG; METABOLISM; METHODOLOGY; METHYLATION; RESPIRATORY TRACT MUCOSA;

AMINO ACIDS; ARGININE; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; HYDROLYSIS; LUNG; METHYLATION; PROTEINS; RESPIRATORY MUCOSA;

EID: 33645960698     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112081     Document Type: Article

References (25)

1. Vallance, P., A. Leone, A. Calver, J. Collier, and S. Moncada. 1992. Accumulation of an endogenous inhibitor of nitric oxide synthesis in chronic renal failure. Lancet 339:572-575.
2. McDermott, J.R. 1976. Studies on the catabolism of Ng-methylarginine, Ng, Ng-dimethylarginine and Ng, Ng-dimethylarginine in the rabbit. Biochem. J. 154:179-184.
3. Cooke, J.P. 2000. Does ADMA cause endothelial dysfunction? Arterioscler. Thromb. Vasc. Biol. 20:2032-2037.
4. MacAllister, R.J., G.S. Whitley, and P. Vallance. 1994. Effects of guanidino and uremic compounds on nitric oxide pathways. Kidney Int. 45:737-742.
5. Boger, R.H., S.M. Bode-Boger, A. Szuba, P.S. Tsao, J.R. Chan, O. Tangphao, T.F. Blaschke, and J.P. Cooke. 1998. Asymmetric dimethylarginine (ADMA): a novel risk factor for endothelial dysfunction: its role in hypercholesterolemia. Circulation 98:1842-1847.
6. Miyazaki, H., H. Matsuoka, J.P. Cooke, M. Usui, S. Ueda, S. Okuda, and T. Imaizumi. 1999. Endogenous nitric oxide synthase inhibitor: a novel marker of atherosclerosis. Circulation 99:1141-1146.
7. Zoccali, C., S. Bode-Boger, F. Mallamaci, F. Benedetto, G. Tripepi, L. Malatino, A. Cataliotti, I. Bellanuova, et al. 2001. Plasma concentration of asymmetrical dimethylarginine and mortality in patients with end-stage renal disease: a prospective study. Lancet 358:2113-2117.
8. Clarke, S. 1993. Protein methylation. Curr. Opin. Cell Biol. 5:977-983.
9. McBride, A.E. and P.A. Silver. 2001. State of the arg: protein methylation at arginine comes of age. Cell 106:5-8.
10. Boisvert, F.M., J. Cote, M.C. Boulanger, and S. Richard. 2003. A proteomic analysis of arginine-methylated protein complexes. Mol. Cell. Proteomics 2:1319-1330.
11. Ong, S.E., G. Mittler, and M. Mann. 2004. Identifying and quantifying in vivo methylation sites by heavy methyl SILAC. Nat. Methods 1:119-126.
12. Boger, R.H., K. Sydow, J. Borlak, T. Thum, H. Lenzen, B. Schubert, D. Tsikas, and S.M. Bode-Boger. 2000. LDL cholesterol upregulates synthesis of asymmetrical dimethylarginine in human endothelial cells: involvement of S-adenosylmethionine-dependent methyltransferases. Circ. Res. 87:99-105.
13. Vallance, P. and J. Leiper. 2004. Cardiovascular biology of the asymmetric dimethylarginine:dimethylarginine dimethylaminohydrolase pathway. Arterioscler. Thromb. Vasc. Biol. 24:1023-1030.
14. Teerlink, T. 1994. Derivatization of post-translationally modified amino acids. J. Chromatogr. B Biomed. Appl. 659:185-207.
15. Pettersson, A., L. Uggla, and V. Backman. 1997. Determination of dimethylated arginines in human plasma by high-performance liquid chromatography. J. Chromatogr. B Biomed. Sci. Appl. 692:257-262.
16. Pi, J., Y. Kumagai, G. Sun, and N. Shimojo. 2000. Improved method for simultaneous determination of L-arginine and its mono-and dimethylated metabolites in biological samples by high-performance liquid chromatography. J. Chromatogr. B Biomed. Sci. Appl. 742:199-203.
17. Teerlink, T., R.J. Nijveldt, S. de Jong, and P.A. van Leeuwen. 2002. Determination of arginine, asymmetric dimethylarginine, and symmetric dimethylarginine in human plasma and other biological samples by high-performance liquid chromatography. Anal. Biochem. 303:131-137.
18. Heresztyn, T., M.I. Worthley, and J.D. Horowitz. 2004. Determination of L-arginine and NG, NG - and NG, NG′ -dimethyl- L-arginine in plasma by liquid chromatography as AccQ-Fluor fluorescent derivatives. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 805:325-329.
19. Unceta, N., A.S. Vicente, M.A. Goicolea, J. Salles, and R.J. Barrio. 2002. Determination of methylarginines in human plasma by HPLC with pre-column derivatization using napthalenedicarboxaldehyde as fluorogenic agent. J. Sep. Sci. 25:665-670.
20. Nonaka, S., M. Tsunoda, K. Imai, and T. Funatsu. 2005. High-performance liquid chromatographic assay of N(G)-monomethyl-L-arginine, N(G),N(G)-dimethyl- L-arginine, and N(G),N(G)′-dimethyl- L-arginine using 4-fluoro-7-nitro-2, 1,3-benzoxadiazole as a fluorescent reagent. J. Chromatogr. A. 1066:41-45.
21. Causse, E., N. Siri, J.F. Arnal, C. Bayle, P. Malatray, P. Valdiguie, R. Salvayre, and F. Couderc. 2000. Determination of asymmetrical dimethylarginine by capillary electrophoresis-laser-induced fluorescence. J. Chromatogr. B Biomed. Sci. Appl. 741:77-83.
22. Vishwanathan, K., R.L. Tackett, J.T. Stewart, and M.G. Bartlett. 2000. Determination of arginine and methylated arginines in human plasma by liquid chromatography-tandem mass spectrometry. J. Chromatogr. B Biomed. Sci. Appl. 748:157-166.
23. Huang, L.F., F.Q. Guo, Y.Z. Liang, B.Y. Li, and B.M. Cheng. 2004. Simultaneous determination of L-arginine and its mono- and dimethylated metabolites in human plasma by high-performance liquid chromatography-mass spectrometry. Anal. Bioanal. Chem. 382:643-649.
24. Pawlak, M.R., C.A. Scherer, J. Chen, M.J. Roshon, and H.E. Ruley. 2000. Arginine N-methyltransferase 1 is required for early post-implantation mouse development, but cells deficient in the enzyme are viable. Mol. Cell. Biol. 20:4859-4869.
25. Deibler, G.E. and R.E. Martenson. 1973. Determination of methylated basic amino acids with the amino acid analyzer. Application to total acid hydrolyzates of myelin basic proteins. J. Biol. Chem. 248:2387-2391.