Growth and metabolism of rCHO cells in glucose-limited fed batch culture

Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities

Volumn 20, Issue 1, 2006, Pages 74-78

  Gao, Liang ^a   You, Lei ^a   Zhu, Ming Long ^a   Tan, Wen Song ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Batch culture; CHO cells; Fed batch culture; Glucose limited

Indexed keywords

ANIMAL CELL CULTURE; BIOREACTORS; GLUCOSE; GROWTH KINETICS; METABOLISM;

FED-BATCH CULTURE; GLUCOSE-LIMITED; RECOMBINANT CHINESE HAMSTER OVARY (RCHO) CELLS;

BATCH CELL CULTURE;

EID: 33645925387     PISSN: 10039015     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. ZHANG Li, SHEN Hong, ZHANG Yuan-xing. Fed-batch culture of hybridoma cells in serum-free medium using an optimized feeding strategy[J]. Journal of Chemical Technology and Biotechnology, 2004, 79(2): 171-181.
2. TANG Jun, ZHI Yong-hong, YE Qin. Metabolic flux distribution in batch cultivation of Candida krusei for glycerol production[J]. J Chem Eng of Chinese Univ, 2002, 16(1): 58-63.
3. ZHOU Ya-jin, TAN Wen-song, ZHOU Jiao, et al. On-line measurement of oxygen uptake rate in the cultivation of Vero cells using the dynamic method[J]. Chinese Journal of Biotechnology, 2000, 16(4): 525-527.
4. Christopher C W, Kohlbacher M S, Amos H. Transport of sugars in check-embryo fibroblasts. Evidence for a low-affinity system and a high-affinity system for glucose transport[J]. Biochem J, 1976, 158(2): 439-450.
5. Fiechter A, Gmunder F K. Metabolic control of glucose degradation in yeast and tumor cells[J]. Adv Biochemical Eng, 1989, 39: 1-28.
6. Nadeau I, Sabatie J, Koehl M, et al. Human 293 cell metabolism in low glutamine-supplied culture: Interpretation of metabolic changes through metabolic flux analysis[J]. Metabolic Engineering, 2000, 2(4): 277-292.
7. Ljunggren J, Haggstrom L. Catabolic control of hybridoma cells by glucose and glutamine limited fed batch cultures[J]. Biotechnol Bioeng, 1994, 44(7): 808-818.