Point mutations within the LdNT2 nucleoside transporter gene from Leishmania donovani confer drug resistance and transport deficiency

International Journal of Biochemistry and Cell Biology

Volumn 38, Issue 7, 2006, Pages 1221-1229

  Galazka, Jon ^a   Carter, Nicola S ^a   Bekhouche, Sabih ^a   Arastu Kapur, Shirin ^a   Ullman, Buddy ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Genetics; Leishmania; Nucleosides; Purines; Transporter

Indexed keywords

FORMYCIN B; MUTANT PROTEIN; NT2 PROTEIN; NUCLEOSIDE TRANSPORTER; UNCLASSIFIED DRUG;

ARTICLE; CELL CLONE; CELL SURFACE; CODON; CONTROLLED STUDY; DRUG SENSITIVITY; ENDOPLASMIC RETICULUM; HETEROZYGOTE; LEISHMANIA DONOVANI; MUTANT; NONHUMAN; NULL ALLELE; OPEN READING FRAME; PHENOTYPE; POINT MUTATION; PROTEIN DOMAIN; PROTEIN FUNCTION;

ANIMALS; CARRIER PROTEINS; DRUG RESISTANCE; LEISHMANIA DONOVANI; NUCLEOSIDE TRANSPORT PROTEINS; POINT MUTATION; PROTEIN TRANSPORT; PROTOZOAN PROTEINS; SUBSTRATE SPECIFICITY;

EUKARYOTA; LEISHMANIA DONOVANI; PROTOZOA;

EID: 33645893197     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biocel.2005.12.016     Document Type: Article

References (18)

1. Arastu-Kapur S., Ford E., Ullman B., and Carter N.S. Functional analysis of an inosine-guanosine transporter from Leishmania donovani. The role of conserved residues, aspartate 389 and arginine 393. Journal of Biological Chemistry 278 35 (2003) 33327-33333
2. Arastu-Kapur S., Arendt C.S., Purnat T., Carter N.S., and Ullman B. Second-site suppression of a non-functional mutation within the Leishmania donovani inosine-guanosine transporter. Journal of Biological Chemistry 280 3 (2005) 2213-2219
3. Aronow B., Kaur K., McCartan K., and Ullman B. Two high affinity nucleoside transporters in Leishmania donovani. Molecular Biochemical Parasitology 22 1 (1987) 29-37
4. Briggs G.E., and Haldane J.B.S. A note on the kinetics of enzyme action. Biochemical Journal (1925) 338-339
5. Carson D.A., and Chang K.P. Phosphorylation and anti-leishmanial activity of formycin B. Biochemical and Biophysical Research Communications 100 3 (1981) 1377-1383
6. Carter N.S., Drew M.E., Sanchez M., Vasudevan G., Landfear S.M., and Ullman B. Cloning of a novel inosine-guanosine transporter gene from Leishmania donovani by functional rescue of a transport-deficient mutant. Journal of Biological Chemistry 275 27 (2000) 20935-20941
7. Carter N.S., Rager N., and Ullman B. Purine and pyrimidine transport and metabolism. In: Marr J.J., Nilsen T.W., and Komuniecki R.W. (Eds). Molecular Medical Parasitology vol. 1 (2003), Elsevier Science Ltd., London 197-223
8. Clayton C., Adams M., Almeida R., Baltz T., Barrett M., Bastien P., et al. Genetic nomenclature for Trypanosoma and Leishmania. Molecular and Biochemical Parasitology 97 1-2 (1998) 221-224
9. Ha D.S., Schwarz J.K., Turco S.J., and Beverley S.M. Use of the green fluorescent protein as a marker in transfected Leishmania. Molecular and Biochemical Parasitology 77 1 (1996) 57-64
10. Iovannisci D.M., and Ullman B. High efficiency plating method for Leishmania promastigotes in semidefined or completely-defined medium. Journal of Parasitology 69 4 (1983) 633-636
11. Iovannisci D.M., Kaur K., Young L., and Ullman B. Genetic analysis of nucleoside transport in Leishmania donovani. Molecular and Cellular Biology 4 6 (1984) 1013-1019
12. Mikus J., and Steverding D. A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue. Parasitology International 48 3 (2000) 265-269
13. Rizvi F.S., Ouaissi M.A., Marty B., Santoro F., and Capron A. The major surface protein of Leishmania promastigotes is a fibronectin-like molecule. European Journal of Immunology 18 3 (1988) 473-476
14. Robinson K.A., and Beverley S.M. Improvements in transfection efficiency and tests of RNA interference (RNAi) approaches in the protozoan parasite Leishmania. Molecular and Biochemical Parasitology 128 2 (2003) 217-228
15. SenGupta D.J., Lum P.Y., Lai Y., Shubochkina E., Bakken A.H., Schneider G., et al. A single glycine mutation in the equilibrative nucleoside transporter gene, hENT1, alters nucleoside transport activity and sensitivity to nitrobenzylthioinosine. Biochemistry 41 5 (2002) 1512-1519
16. Valdes R., Vasudevan G., Conklin D., and Landfear S.M. Transmembrane domain 5 of the LdNT1.1 nucleoside transporter is an amphipathic helix that forms part of the nucleoside translocation pathway. Biochemistry 43 21 (2004) 6793-6802
17. Vasudevan G., Carter N.S., Drew M.E., Beverley S.M., Sanchez M.A., Seyfang A., et al. Cloning of Leishmania nucleoside transporter genes by rescue of a transport-deficient mutant. Proceedings of the National Academy of Sciences USA 95 17 (1998) 9873-9878
18. Vasudevan G., Ullman B., and Landfear S.M. Point mutations in a nucleoside transporter gene from Leishmania donovani confer drug resistance and alter substrate selectivity. Proceedings of the National Academy of Sciences USA 98 11 (2001) 6092-6097