Molecular characterization of Erwinia amylovora strains from different host plants through RFLP analysis and sequencing of hrpN and dspA/E genes

Plant Pathology

Volumn 54, Issue 6, 2005, Pages 789-798

  Giorgi, S ^a   Scortichini, M ^a  

^a CRA Istituto Sperimentale Per la Frutticoltura   (Italy)

Author keywords

Amelanchier; Erwinia pyrifoliae; Fireblight; Gene sequencing; Rosaceae; Rubus

Indexed keywords

BACTERIAL DISEASE; BACTERIUM; DNA FINGERPRINTING; ENZYME ACTIVITY; INOCULATION; PATHOGEN; PLANT;

AMELANCHIER; ERWINIA; ERWINIA AMYLOVORA; ERWINIA PYRIFOLIAE; MALOIDEAE; PYRUS COMMUNIS; RUBUS;

EID: 33645890834     PISSN: 00320862     EISSN: 13653059     Source Type: Journal    
DOI: 10.1111/j.1365-3059.2005.01247.x     Document Type: Article

References (25)

1. Barny M-A, 1995. Erwinia amylovora hrpN mutants, blocked in harpin synthesis, express a reduced virulence on host plants and elicit variable hypersensitivity reaction on tobacco. European Journal of Plant Pathology 101, 333 40.
2. Barny M-A Guinebretiére M-H Marcais B Coissac E Paulin J-P Laurent J, 1990. Cloning of a large gene cluster involved in Erwinia amylovora CFBP 1430 virulence. Molecular Microbiology 4, 777 86.
3. Bauer DW Beer SV, 1991. Further characterization of an hrp gene cluster of Erwinia amylovora. Molecular Plant-Microbe Interactions 4, 493 9.
4. Bogdanove AJ Kim JF Wei Z Kolchinsky P Charkowsky AO Conlin AK Collmer A Beer SV, 1998. Homology and functional similarity of an hrp-linked pathogenicity locus, dspEF, of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomato. Proceedings of the National Academy of Sciences, USA 95, 1325 30.
5. Bogdanove AJ Kim JF Beer SV, 2000. Disease-specific genes of Erwinia amylovora: keys to understanding pathogenesis and potential targets for disease control. In : Vanneste JL, ed. Fire Blight: The Disease and its Causative Agent. Wallingford, UK : CAB International, 163 77.
6. Bugert P Geider K, 1995. Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora. Molecular Microbiology 15, 917 33.
7. Evans IR, 1996. Fire blight of raspberries in Alberta. Acta Horticulturae 411, 69 72.
8. Gardner JM Kado CI, 1972. Comparative base sequence homologies of the deoxyribonucleic acids of Erwinia species and other Enterobacteriaceae. International Journal of Systematic Bacteriology 22, 201 8.
9. Gaudriault S Malandrin L Paulin J-P Barny M-A, 1997. DspA, an essential pathogenicity factor of Erwinia amylovora showing homology with AvrE of Pseudomonas syringae, is secreted via the Hrp secretion pathway in a DspB-dependent way. Molecular Microbiology 26, 1057 69.
10. Higgins D Thompson J Gibson T Thompson JD Higgins DG Gibson TJ, 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673 80.
11. Jin G Hu W Brown I McGhee G Hart P Jones AL He S-Y, 2001. Visualization of secreted Hrp and Avr proteins along the Hrp pilus during type III secretion in Erwinia amylovora and Pseudomonas syringae. Molecular Microbiology 40, 1129 39.
12. Jock S Geider K, 2004. Molecular differentiation of Erwinia amylovora strains from North America and of two Asian pear pathogens by analyses of PFGE patterns and hrpN genes. Environmental Microbiology 6, 480 90.
13. Kim JF Beer SV, 1998. HrpW of Erwinia amylovora, a new harpin that contains a domain homologous to pectate lyases of a distinct class. Journal of Bacteriology 180, 5203 10.
14. Kim JF Beer SV, 2000. hrp genes and harpins of Erwinia amylovora: a decade of discovery. In : Vanneste JL, ed. Fire Blight: The Disease and its Causative Agent. Wallingford, UK : CAB International, 141 61.
15. Kim WS Gardan L Rhim SL Geider K, 1999. Erwinia pyrifoliae sp. nov., a novel pathogen affecting Asian pear trees (Pyrus pyrifolia). International Journal of Systematic Bacteriology 49, 899 906.
16. King EO Raney MK Ward DE, 1954. Two simple media for the demonstration of pyiocianin and fluorescin. Journal of Laboratory and Clinical Medicine 44, 301 7.
17. Klement Z, 1982. Hypersensitivity. In : Mount MS Lacy GS, eds. Phytopathogenic Prokaryotes, Vol. 2. New York, USA : Academic Press, 149 77.
18. Lelliott RA Stead DE, 1987. Methods for the diagnosis of bacterial diseases of plants. In : Preece TF, ed. Methods in Plant Pathology, Vol. 2. Oxford, UK : Blackwell Scientific.
19. McGhee GC Schnabel EL Stein K-M Jones B Stromberg VK Lacy GH Jones AL, 2002. Relatedness of chromosomal and plasmid DNAs of Erwinia pyrifoliae and Erwinia amylovora. Applied and Environmental Microbiology 68, 6182 92.
20. McManus PS Jones AL, 1995. Genetic fingerprinting of Erwinia amylovora strains isolated from tree-fruit crops and Rubus spp. Phytopathology 85, 1547 53.
21. Ries SM Otterbacker AG, 1977. Occurrence of fire blight on thornless blackberry in Illinois. Plant Disease Reporter 61, 232 5.
22. Smith JJ Offord LC Holderness M Saddler GJ, 1995. Genetic diversity of Burkholderia solanacearum (synonym: Pseudomonas solanacearum) race 3 in Kenya. Applied and Environmental Microbiology. 61, 4263 8.
23. Starr MR Cardona C Folsom D, 1951. Bacterial fire blight of raspberry. Phytopathology 41, 915 9.
24. Venisse J-S Barny M-A Paulin J-P Brisset M-N, 2003. Involvement of three pathogencity factors of Erwinia amylovora in the oxidative stress associated with compatible interaction in pear. FEBS Letters 537, 198 202.
25. Wei ZH Laby RJ Zumoff CH Bauer DW He S-Y Collmer A Beer SV, 1992. Harpin, elicitor of the hypersensitivity response produced by the plant pathogen Erwinia amylovora. Science 257, 85 8.