In vitro maturation and fertilization of porcine oocytes after a 48 h culture in roscovitine, an inhibitor of p34cdc2/cyclin B kinase

Animal Reproduction Science

Volumn 92, Issue 3-4, 2006, Pages 321-333

  Romar, Raquel ^a,b   Funahashi, Hiroaki ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b UNIVERSITY OF MURCIA   (Spain)

Author keywords

Glutathione; In vitro fertilization; In vitro maturation; Pig porcine; Roscovitine

Indexed keywords

CYCLIN B; CYCLIN DEPENDENT KINASE; CYCLIN DEPENDENT KINASE ACTIVATING KINASE; CYCLIN-DEPENDENT KINASE-ACTIVATING KINASE; GLUTATHIONE; PROTEIN KINASE INHIBITOR; PURINE DERIVATIVE; ROSCOVITINE;

ANIMAL; ANIMAL DISEASE; ARTICLE; DRUG ANTAGONISM; DRUG EFFECT; EMBRYO CULTURE; EMBRYO DEVELOPMENT; EMBRYO INDUCTION; ENZYMOLOGY; FEMALE; FERTILIZATION IN VITRO; MALE; METABOLISM; OOCYTE; PHYSIOLOGY; RANDOMIZATION; SWINE; TIME;

ANIMALS; CYCLIN B; CYCLIN-DEPENDENT KINASES; EMBRYO CULTURE TECHNIQUES; EMBRYONIC DEVELOPMENT; EMBRYONIC INDUCTION; FEMALE; FERTILIZATION IN VITRO; GLUTATHIONE; MALE; OOCYTES; PROTEIN KINASE INHIBITORS; PURINES; RANDOM ALLOCATION; SWINE; TIME FACTORS;

SUIDAE; SUS SCROFA;

EID: 33645840168     PISSN: 03784320     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.anireprosci.2005.04.017     Document Type: Article

References (32)

1. Abeydeera L.R. In vitro fertilization and embryo development in pigs. Reprod. Suppl. 58 (2001) 159-173
2. Anderson M.E. Determination of glutathione and glutathione disulfide in biological samples. In: Meister A. (Ed). Methods in Enzymology: Glutamate, Glutamine, Glutathione and Related Compounds 113 (1985), Academic Press, New York 548-555
3. Brad A.M., Bormann C.L., Swain J.E., Durkin R.E., Johnson A.E., Clifford A.L., and Krisher R.L. Glutathione and adenosine triphosphate content of in vivo and in vitro matured porcine oocytes. Mol. Reprod. Dev. 64 (2003) 492-498
4. Coy P., Ruiz S., Romar R., Campos I., and Gadea J. Maturation, fertilization and complete development of porcine oocytes matured under different systems. Theriogenology 51 (1999) 799-812
5. Coy P., and Romar R. In vitro production of pig embryos. A point of view. Reprod. Fertil. Dev. 14 (2002) 275-286
6. Coy P., Romar R., Payton R.R., McCann L., Saxton A.M., and Edwards J.L. Maintenance of meiotic arrest in bovine oocytes using the S-enantiomer of roscovitine: Effects on maturation, fertilization and subsequent embryo development in vitro. Reproduction 129 (2005) 19-26
7. Coy, P., Romar, R., Ruiz, S., Canovas, S., Gadea, J., Garcia, F.A., Matás, C., 2005b. Birth of piglets after transferring of in vitro produced embryos pre-cultured with roscovitine. Reproduction. 63, 747-775.
8. Funahashi H., Cantley T., and Day B.N. Different hormonal requirements of pig oocyte-cumulus complexes during maturation in vitro. J. Reprod. Fertil. 101 (1994) 159-165
9. Funahashi H., Cantley T.C., Stumpf T.T., Terlouw S.L., and Day B.N. In vitro development of in vitro-matured porcine oocytes following chemical activation or in vitro fertilization. Biol. Reprod. 50 (1994) 1072-1077
10. Funahashi H., Stumpf T.T., Cantley T.C., Kim N.H., and Day B.N. Pronuclear formation and intracellular glutathione content of in vitro-matured porcine oocytes following in vitro fertilization and/or electrical activation. Zygote 3 (1995) 273-281
11. Funahashi H., and Day B.N. Advances in in vitro production of pig embryos. J. Reprod. Fertil. Suppl. 52 (1997) 271-283
12. Funahashi H., Cantley T.C., and Day B.N. Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic AMP improves developmental competence following in vitro fertilization. Biol. Reprod. 57 (1997) 49-53
13. Funahashi H., and Nagai T. Regulation of in vitro penetration of frozen-thawed boar spermatozoa by caffeine and adenosine. Mol. Reprod. Dev. 58 (2001) 424-431
14. Funahashi H., and Romar R. Reduction of the incidence of polyspermic penetration into porcine oocytes by pretreatment of fresh spermatozoa with adenosine and a transient co-incubation of the gametes to caffeine. Reproduction 128 (2004) 789-800
15. Ju J.-C., Tsay C., and Ruan C.-W. Alterations and reversibility in the chromatin, cytoskeleton and development of pig oocytes treated with roscovitine. Mol. Reprod. Dev. 64 (2003) 482-491
16. Krischek C., and Meinecke B. Roscovitine, a specific inhibitor of cyclin-dependent protein kinases, reversibly inhibits chromatin condensation during in vitro maturation of porcine oocytes. Zygote 9 (2001) 309-316
17. Le Beux G., Richard F.J., and Sirard M.-A. Effect of cycloheximide, 6-DMAP, roscovitine and butyrolactone I on resumption of meiosis in porcine oocytes. Theriogenology 60 (2003) 1049-1058
18. Lonergan P., Faerge I., Hyttel P.M., Boland M., and Fair T. Ultrastructural modifications in bovine oocytes maintained in meiotic arrest in vitro using roscovitine or butyrolactone. Mol. Reprod. Dev. 64 (2003) 369-378
19. Marchal R., Tomanek M., Terqui M., and Mermillod P. Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes. Mol. Reprod. Dev. 60 (2001) 65-73
20. Matás C., Coy P., Romar R., Marco M., Gadea J., and Ruiz S. Effect of sperm preparation method on in vitro fertilisation in pigs. Reproduction 125 (2003) 133-141
21. McClue S.J., Blake D., Clarke R., Cowan A., Cummings L., Fischer P.M., MacKenzie M., Melville J., Stewart K., Wang S., Zhelev N., Zheleva D., and Lane D.P. In vitro and in vivo antitumor properties of the cyclin dependent kinase inhibitor CYC202 (R-roscovitine). Int. J. Cancer 102 (2002) 463-468
22. Meijer L., and Raymond E. Roscovitine and other purines as kinase inhibitors. From starfish oocytes to clinical trials. Acc. Chem. Res. 36 (2003) 417-425
23. Mermillod P., Tomanek M., Marchal R., and Meijer L. High developmental competence of cattle oocytes maintained at the germinal vesicle stage for 24 h in culture by specific inhibition of MPF kinase activity. Mol. Reprod. Dev. 55 (2000) 89-95
24. Payton R.R., Romar R., Coy P., Saxton A.M., Lawrence J.L., and Edwards J.L. Susceptibility of bovine germinal vesicle-stage oocytes from antral follicles to direct effects of heat stress in vitro. Biol. Reprod. 71 (2004) 1303-1308
25. Petters R.M., and Wells K.D. Culture of pig embryos. J. Reprod. Fertil. Suppl. 48 (1993) 61-73
26. Schoevers, E.J., Bevers, M.M., Roelen, B.A.J., Colenbrander, B., 2005. Nuclear and cytoplasmic maturation of sow oocytes are not synchronized by specific meiotic inhibition with roscovitine during in vitro maturation. Theriogenology 63, 1111-1130.
27. Sutovsky P., and Schatten G. Depletion of glutathione during bovine oocyte maturation reversibly blocks the decondensation of the male pronucleus and pronuclear apposition during fertilization. Biol. Reprod. 56 (1997) 1503-1512
28. Sutton M.L., Gilchrist R.B., and Thompson J.G. Effects of in-vivo and in-vitro environments on the metabolism of the cumulus-oocyte complex and its influence on oocyte developmental capacity. Hum. Reprod. Update 9 (2003) 35-48
29. Vigneron C., Nuttinck F., Perreau C., Reinaud P., Charpigny G., and Mermillod P. Effect of roscovitine, a cdk1 inhibitor, and of the presence of oocyte on bovine cumulus cell expansion and cyclooxygenase-2 expression. Mol. Reprod. Dev. 65 (2003) 114-121
30. Wehrend A., and Meinecke B. Kinetics of meiotic progression, M-phase promoting factor (MPF) and mitogen-activated protein kinase (MAP kinase) activities during in vitro maturation of porcine and bovine oocytes: species specific differences in the length of the meiotic stages. Anim. Reprod. Sci. 66 (2001) 175-184
31. Ye J., Flint P.F., Campbell K.H.S., and Luck M.R. Synchronization of porcine oocyte meiosis using cycloheximide and its application to the study of regulation by cumulus cells. Reprod. Fertil. Dev. 14 (2002) 433-442
32. Yoshida M., Ishigaki K., Nagai T., Chikyu M., and Pursel V.G. Glutathione concentration during maturation and after fertilization in pig oocytes: relevance to the ability of oocytes to form male pronucleus. Biol. Reprod. 49 (1993) 89-94