The apoptotic effect of brucine from the seed of strychnos nux-vomica on human hepatoma cells is mediated via Bcl-2 and Ca2+ involved mitochondrial pathway

Toxicological Sciences

Volumn 91, Issue 1, 2006, Pages 59-69

  Deng, Xukun ^a,b   Yin, Fangzhou ^a   Lu, Xiaoyu ^a   Cai, Baochang ^a   Yin, Wu ^c  

^a NANJING UNIVERSITY OF CHINESE MEDICINE   (China)

^b SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES   (China)

^c NANJING UNIVERSITY   (China)

Author keywords

Apoptosis; Bcl 2; Brucine; Ca2+; Caspases; HepG2 cells

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ALKALOID; BRUCINE; CALCIUM ION; CASPASE 3; CASPASE 9; CYCLOSPORIN A; CYTOCHROME C; DNA FRAGMENT; ISOSTRYCHNINE; PHOSPHATIDYLSERINE; PROTEIN BCL 2; STRYCHNINE; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CALCIUM CELL LEVEL; CELL CYCLE ARREST; CELL SIZE; CELL STRAIN HEPG2; CELL VIABILITY; CHINESE MEDICINE; CONTROLLED STUDY; DRUG MECHANISM; DRUG STRUCTURE; ENZYME ACTIVATION; ENZYME INHIBITION; ENZYME RELEASE; GENE OVEREXPRESSION; HEPATOMA CELL; HUMAN; HUMAN CELL; LIVER CANCER; MEMBRANE DEPOLARIZATION; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; PLANT SEED; PROTEIN DEGRADATION; STRYCHNOS NUX VOMICA;

APOPTOSIS; CALCIUM; CARCINOMA, HEPATOCELLULAR; CASPASE 3; CASPASES; CELL LINE, TUMOR; CYCLIC N-OXIDES; CYTOCHROMES C; ENZYME ACTIVATION; HUMANS; LIVER NEOPLASMS; MITOCHONDRIA, LIVER; PROTO-ONCOGENE PROTEINS C-BCL-2; SEEDS; STRYCHNINE; STRYCHNOS NUX-VOMICA;

STRYCHNOS NUX-VOMICA; STRYCHNOS TOXIFERA;

EID: 33645831566     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfj114     Document Type: Article

References (30)

1. Assaf, H., Azouri, H., and Pallardy, M. (2004). Ochratoxin A induces apoptosis in human lymphocytes through down regulation of Bcl-xL. Toxicol. Sci. 79, 335-344.
2. Bisset, N. G., and Phillipson, J. D. (1971). The tertiary alkaloids of some Asian species of strychnos. J. Pharm. Pharmacol. 23, 244S.
3. Cai, B. C., Hattori, M., and Namba, T. (1990). Processing of nux vomica. II. Changes in alkaloid composition of the seeds of Strychnos nux-vomica L. on traditional drug-processing. Chem. Pharm. Bull. 38, 1295-1298.
4. Cai, B. C., He, Y.W., Ding, H. F., Ma, C., and Wu, H. (1994). Determination of alkaloids and comparison of the acute toxicity of differently processed products of the seeds of Strychnos nux-vomica L. J. Chin. Herb. Med. 19, 598-600, 638.
5. Cai, B. C., Wang, T. S., Kurokawa, M., Shiraki, K., and Hattori, M. (1998). Cytotoxicities of alkaloids from processed and unprocessed seeds of Strychnos nux-vomica L. Acta Pharmacol. Sin. 19, 425-428.
6. Cai, R. L., Meng, W., Lu, H. Y., Lin, W. Y., Jiang, F., and Shen, F. M. (2003). Segregation analysis of hepatocellular carcinoma in a moderately high-incidence area of East China. World J. Gastroenterol. 9, 2428-2432.
7. Dua, P., and Gude, R. P. (2005). Antiproliferative and antiproteolytic activity of pentoxifylline in cultures of B16F10 melanoma cells. Cancer Chemother. Pharmacol. 6, 1-8.
8. Hofmann, K., Bucher, P., and Tschopp, J. (1997). The CARD domain: A new apoptotic signaling motif. Trends Biochem. Sci. 22, 155-156.
9. Kim, A. J., Shi, Y., Austin, R. C., and Werstuck, G. H. (2003). Valproate protects cells from ER stress-induced lipid accumulation and apoptosis by inhibiting glycogen synthase kinase-3. J. Cell Sci. 118, 89-99.
10. Kluck, R. M., Bossy-Wetzel, E., Green, D. R., and Newmeyer, D. D. (1997). The release of cytochrome c from mitochondria: A primary site for Bcl-2 regulation of apoptosis. Science 275, 1132-1136.
11. Kwon, K. B., Yoo, S. J., Ryu, D. G., Yang, J. Y., Rho, H. W., Kim, J. S., Park, J. W., Kim, H. R., and Park, B. H. (2002). Induction of apoptosis by diallyl disulfide through activation of caspase-3 in human leukemia HL-60 cells. Biochem. Pharmacol. 63, 41-47.
12. 2-induced apoptosis of alveolar type II cells. J. Cell. Biochem. 94, 433-445.
13. Li, H. L., Zhang, H.W., Chen, D. D., Zhong, L., Ren, X. D., and Tu, R. (2002). JTE-522, a selective COX-2 inhibitor, inhibits cell proliferation and induces apoptosis in RL95-2 cells. Acta Pharmacol. Sin. 23, 631-637.
14. Li, P., Nijhawan, D., Budihardjo, I., Srinivasula, S. M., Ahmad, M., Alnemri, E. S., and Wang, X. (1997). Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91, 479-489.
15. Li, X. H., Li, J. J., and Zhang, H. W. (2003). Nimesulide inhibits tumor growth in mice implanted hepatoma: Overexpression of Bax over Bcl-2. Acta Pharmacol. Sin. 24, 1045-1050.
16. Liu, X. S., Kim, C. N., Yang, J., Jemmerson, R., and Wang, X. D. (1996). Induction of apoptotic program in cell-free extracts: Requirement for dATP and cytochrome c. Cell 86, 147- 157.
17. 2+ from endoplasmic reticulum stores and mitochondrial apoptosis during stress-induced cell death. J. Biol. Chem. 280, 23829-23836.
18. McConkey, D. J., and Orrenius, S. (1997). The role of calcium in the regulation of apoptosis. Biochem. Biophys. Res. Commun. 239, 357-366.
19. Nagata, S., Nagase, H., Kawane, K., Mukae, N., and Fukuyama, F. (2003). Degradation of chromosomal DNA during apoptosis. Cell Death Differ. 10, 108-116.
20. Ramonede, B. M., and Perez-Tomas, R. P. (2002). Activation of protein kinase C for protection of cells against apoptosis induced by the immunosuppressor prodigiosin. Biochem. Pharmacol. 63, 463-469.
21. Sei, Y., Fossom, L., Goping, G., Skolnick, P., and Basile, A. S. (1998). Quinolinic acid protects rat cerebellar granule cells from glutamate-induced apoptosis. Neurosci. Lett. 241, 180-184.
22. Selivanova, G. (2004). p53: Fighting cancer. Curr. Cancer Drug Targets 4, 385-402.
23. Sheng, H., Shao, J., and Kirkland, S. (1997). Inhibition of human colon cancer cell growth by selective inhibition of cyclooxygenase-2. J. Clin. Invest. 99, 2254-2259.
24. Smets, L. A. (1994). Programmed cell death (apoptosis) and response to anticancer drugs. Anticancer Drugs 5, 3-9.
25. Suzuki, M., Harada, S., Owaribe, K., and Yaoita, H. (1996). Intracellular ionic changes induced by bullous pemphigoid IgG subclasses. Autoimmunity 23, 181-197.
26. Watson, A. J. (1995). Manipulation of cell death - the development of novel strategies for the treatment of gastrointestinal disease. Aliment. Pharmacol. Ther. 9, 215-226.
27. Xu, X. Y., Cai, B. C., Pan, Y., and Wang, T. S. (2003). Pharmacokinetics of the alkaloids from the processed seeds of Strychnos nux-vomica in rats. Yao Xue Xue Bao 38, 458-461.
28. Yang, J., Liu, X., Bhalla, K., Kim, C. N., Ibrado, A. M., Cai, J., Peng, T., Jones, D. P., and Wang, X. (1997). Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked. Science 275, 1129-1132.
29. Yin, W., Jiang, G., Takeyasu, K., and Zhou, X. (2003a). Stimulation of Na, KATPase by low potassium is dependent on transferrin. J. Membr. Biol. 193, 177-184.
30. Yin, W., Wang, T. S., Yin, F. Z., and Cai, B. C. (2003b). Analgesic and anti-inflammatory properties of brucine and brucine N-oxide extracted from seeds of Strychnox nux-vomica. J. Ethnopharmacol. 88, 205-214.