The Saccharomyces cerevisiae Sae2 protein negatively regulates DNA damage checkpoint signalling

EMBO Reports

Volumn 7, Issue 2, 2006, Pages 212-218

  Clerici, Michela ^a   Mantiero, Davide ^a   Lucchini, Giovanna ^a   Longhese, Maria Pia ^a  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

Author keywords

Checkpoint; Mec1; MRX; Sae2; Tel1

Indexed keywords

DNA; DOUBLE STRANDED DNA; PROTEIN SAE2; SACCHAROMYCES CEREVISIAE PROTEIN; UNCLASSIFIED DRUG; DEOXYRIBONUCLEASE; EXODEOXYRIBONUCLEASE; FUNGAL DNA; FUNGAL PROTEIN; MEC1 PROTEIN, S CEREVISIAE; MRE11 PROTEIN, S CEREVISIAE; PROTEIN SERINE THREONINE KINASE; SAE2 PROTEIN, S CEREVISIAE; SIGNAL PEPTIDE; TEL1 PROTEIN, FUNGAL; TEL1 PROTEIN, S CEREVISIAE;

ARTICLE; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; FUNGAL STRAIN; GENE ACTIVATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEFICIENCY; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; STRAIN DIFFERENCE; CELL CYCLE; CYTOLOGY; DNA REPAIR; FUNGAL GENE; GENETICS; METABOLISM; PHOSPHORYLATION; TIME; WESTERN BLOTTING;

SACCHAROMYCES CEREVISIAE;

BLOTTING, WESTERN; CELL CYCLE; DNA DAMAGE; DNA REPAIR; DNA, FUNGAL; ENDODEOXYRIBONUCLEASES; EXODEOXYRIBONUCLEASES; FUNGAL PROTEINS; GENES, FUNGAL; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SIGNAL TRANSDUCTION; TIME FACTORS;

EID: 33645799075     PISSN: 1469221X     EISSN: 14693178     Source Type: Journal    
DOI: 10.1038/sj.embor.7400593     Document Type: Article

References (19)

1. Baroni E, Viscardi V, Cartagena-Lirola H, Lucchini G, Longhese MP (2004) The functions of budding yeast Sae2 in the DNA damage response require Mec1- and Tel1-dependent phosphorylation. Mol Cell Biol 24: 4151-4165
2. Clerici M, Mantiero D, Lucchini G, Longhese MP (2005) The Saccharomyces cerevisiae Sae2 protein promotes resection and bridging of double-strand break ends. J Biol Chem 280: 38631-38638
3. Ira G et al (2004) DNA end resection, homologous recombination and DNA damage checkpoint activation require CDK1. Nature 431: 1011-1017
4. Keeney S, Kleckner N (1995) Covalent protein-DNA complexes at the 5′ strand termini of meiosis-specific double-strand breaks in yeast. Proc Natl Acad Sci USA 92: 11274-11278
5. Lee SE, Moore JK, Holmes A, Umezu K, Kolodner RD, Haber JE (1998) Saccharomyces Ku70, Mre11/Rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damage. Cell 94: 399-409
6. Lisby M, Barlow JH, Burgess RC, Rothstein R (2004) Choreography of the DNA damage response: Spatiotemporal relationships among checkpoint and repair proteins. Cell 118: 699-713
7. Llorente B, Symington LS (2004) The Mre11 nuclease is not required for 5′ to 3′ resection at multiple HO-induced double-strand breaks. Mol Cell Biol 24: 9682-9694
8. Lobachev KS, Gordenin DA, Resnick MA (2002) The Mre11 complex is required for repair of hairpin-capped double-strand breaks and prevention of chromosome rearrangements. Cell 108: 183-193
9. Longhese MP, Clerici M, Lucchini G (2003) The S-phase checkpoint and its regulation in Saccharomyces cerevisiae. Mutat Res 532: 41-58
10. Nakada D, Matsumoto K, Sugimoto K (2003) ATM-related Tel1 associates with double-strand breaks through an Xrs2-dependent mechanism. Genes Dev 16: 1957-1962
11. Nakada D, Hirano Y, Sugimoto K (2004) Requirement of the Mre11 complex and exonuclease 1 for activation of the Mec1 signaling pathway. Mol Cell Biol 24: 10016-10025
12. Neale MJ, Ramachandran M, Trelles-Sticken E, Scherthan H, Goldman AS (2002) Wild-type levels of Spo11-induced DSBs are required for normal single-strand resection during meiosis. Mol Cell 9: 835-846
13. Paull TT, Gellert M (1998) The 3′ to 5′ exonuclease activity of Mre11 facilitates repair of DNA double-strand breaks. Mol Cell 7: 969-979
14. Pellicioli A, Lee SE, Lucca C, Foiani M, Haber JE (2001) Regulation of Saccharomyces Rad53 checkpoint kinase during adaptation from DNA damage-induced G2/M arrest. Mol Cell 7: 293-300
15. Rattray AJ, McGill CB, Shafer BK, Strathern JN (2001) Fidelity of mitotic double-strand-break repair in Saccharomyces cerevisiae: A role for SAE2/COM1. Genetics 158: 109-122
16. Toczyski DP, Galgoczy DJ, Hartwell LH (1997) CDC5 and CKII control adaptation to the yeast DNA damage checkpoint. Cell 90: 1097-1106
17. Usui T, Ogawa H, Petrini JHJ (2001) A DNA damage response pathway controlled by Tel1 and the Mre11 complex. Mol Cell 7: 1255-1266
18. Vaze MB, Pellicioli A, Lee SE, Ira G, Liberi G, Arbel-Eden A, Foiani M, Haber JE (2002) Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase. Mol Cell 10: 373-385
19. Zou L, Elledge SJ (2003) Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes. Science 300: 1542-1548