Design and synthesis of a photocleavable fluorescent nucleotide 3′-O-allyl-dGTP-PC-Bodipy-FL-510 as a reversible terminator for DNA sequencing by synthesis

Journal of Organic Chemistry

Volumn 71, Issue 8, 2006, Pages 3248-3252

  Meng, Qinglin ^a,b,c   Kim, Dae Hyun ^a,b   Bai, Xiaopeng ^a,b,c   Bi, Lanrong ^a,b   Turro, Nicholas J ^b,c   Ju, Jingyue ^a,b  

^a Department of Neurology and the Taub Institute for Research on Alzheimer's Disease and the Aging Brain   (United States)

^b Fu Foundation School of Engineering and Applied Science   (United States)

^c Howard Hughes Medical Institute   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLYL GROUP; NUCLEOTIDES; SEQUENCING BY SYNTHESIS (SBS);

CATALYSIS; DNA; FLUORESCENCE; POLYMERIZATION; REACTION KINETICS; SYNTHESIS (CHEMICAL);

ORGANIC COMPOUNDS;

3' O ALLYL DEOXYGUANOSINE TRIPHOSPHATE; ALLYL COMPOUND; DEOXYGUANOSINE TRIPHOSPHATE; DNA; DNA POLYMERASE; FLUORESCENT DYE; NUCLEOTIDE DERIVATIVE; PALLADIUM; POLYMER; UNCLASSIFIED DRUG;

ARTICLE; CATALYST; DNA CLEAVAGE; DNA SEQUENCE; FLUORESCENCE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NUCLEOTIDE METABOLISM; PH; QUANTITATIVE ANALYSIS; SYNTHESIS;

BASE SEQUENCE; DEOXYGUANINE NUCLEOTIDES; DNA-DIRECTED DNA POLYMERASE; FLUORESCENT DYES; PHOTOCHEMISTRY;

EID: 33645753463     PISSN: 00223263     EISSN: None     Source Type: Journal    
DOI: 10.1021/jo060300k     Document Type: Article

References (9)

1. Ju, J.; Li, Z.; Edwards, J.; Itagaki, Y. U.S. Patent 6,664,079, 2003.
2. Ruparel, H.; Bi, L.; Li, Z.; Bai, X.; Kim, D. H.; Turro, N. J.; Ju, J. Proc. Natl. Acad. Sci. U.S.A. 2005, 102, 5932-5937.
3. Seela, F.; Driller, H. Nucl. Nucl. 1989, 8, 1-21.
4. Ramzaeva, N.; Seela, F. Hel. Chim. Acta 1995, 78, 1083-1090.
5. Ryu, E. K.; Ross, R. J.; Matsushita, T.; MacCoss, M.; Hong, C. I.; West, C. R. J. Med. Chem. 1982, 25, 1322-1329.
6. Hobbs, F. W. J. Org. Chem. 1989, 54, 3420-3422.
7. Ramasamy, K.; Imarura, N.; Robins, R. K.; Revankar, G. R. J. Heterocycl. Chem. 1988, 25, 1893-1898.
8. Lee, S. E.; Sidorov, A.; Gourlain, T.; Mignet, N.; Thorpe, S. J.; Brazier, J. A.; Dickman, M. J.; Hornby, D. P.; Grasby, J. A.; Williams, D. M. Nucleic Acids Res. 2001, 29, 1565-1573.
9. Seo, T. S.; Bai, X.; Kim, D. H.; Meng, Q.; Shi, S.; Ruparel, H.; Li, Z.; Turro, N. J.; Ju, J. Proc. Natl. Acad. Sci. U.S.A. 2005, 102, 5926-5931.