The relative merits of the tetO2 and tetO7 promoter systems for the functional analysis of heterologous genes in yeast and a compilation of essential yeast genes with tetO2 promoter substitutions

Yeast

Volumn 23, Issue 4, 2006, Pages 325-331

  Wishart, Jill A ^a   Osborn, Michael ^b   Gent, Manda E ^a   Yen, Kuangyu ^a   Vujovic, Zorana ^a   Gitsham, Paul ^a   Zhang, Nianshu ^a   Miller, J Ross ^b   Oliver, Stephen G ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b BABRAHAM INSTITUTE   (United Kingdom)

Author keywords

Essential genes; Functional genomics; Saccharomyces cerevisiae; TetO2; TetO7; Tetracycline regulatable promoter

Indexed keywords

BETA GALACTOSIDASE; FUNGAL DNA; INTERFERON REGULATORY FACTOR 1; TETRACYCLINE; BACTERIAL PROTEIN; CARRIER PROTEIN; TET O RESISTANCE PROTEIN, BACTERIA;

ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; FUNCTIONAL GENOMICS; FUNGAL STRAIN; GENE CONTROL; GENE EXPRESSION; GENETIC SCREENING; HETEROLOGOUS EXPRESSION; NONHUMAN; PHENOTYPIC VARIATION; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; STRAIN DIFFERENCE; FUNGAL GENE; GENE EXPRESSION REGULATION; GENETICS; HUMAN; LACTOSE OPERON; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; POLYMERASE CHAIN REACTION;

SACCHAROMYCES CEREVISIAE;

BACTERIAL PROTEINS; BASE SEQUENCE; CARRIER PROTEINS; DNA, FUNGAL; GENES, FUNGAL; HUMANS; INTERFERON REGULATORY FACTOR-1; LAC OPERON; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT;

EID: 33645743256     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1348     Document Type: Article

References (31)

1. Belli G, Aldea M, Herrero E. 1998a. Functional analysis of yeast essential genes using a promoter substitution cassette and the tetracycline-regulatable dual expression system. Yeast 14: 1127-1138.
2. Belli G, Gari E, Piedrafita L, Aldea M, Herrero E. 1998b. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast. Nucleic Acids Res 26: 942-947.
3. Brown AJ, Plant RJ, Restuhadi F, et al. 2001. Transcript analysis of 1003 novel yeast genes using high-throughput northern hybridizations. EMBO J 20: 3177-3186.
4. Castrillo JI, Oliver SG. 2004. Yeast as a touchstone in post-genomic research: strategies for integrative analysis in functional genomics. J Biochem Mol Biol 37: 93-106.
5. Chervitz SA, Aravind L, Sherlock G, et al. 1998. Comparison of the complete protein sets of worm and yeast: orthology and divergence. Science 282: 2022-2028.
6. Corpet F. 1988. Multiple sequence alignment with hierarchical alignment. Nucleic Acids Res 16: 10881-10890.
7. Doniger SW, Huh J, Fay JC. 2005. Identification of functional transcription factor binding sites using closely related Saccharomyces species. Genome Res 15: 701-709.
8. EUROFAN. http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/ess.html.
9. Foury F, Kucej M. 2002. Yeast mitochondrial biogenesis: a model system for humans? Curr Opin Chem Biol 6: 106-111.
10. Fujita T, Shibuya H, Hotta H, Yamanishi K, Taniguchi T. 1987. Interferon-β gene regulation: tandemly repeated sequences of a synthetic 6 bp oligomer function as a virus-inducible enhancer. Cell 49: 357-367.
11. Gari E, Piedrafita L, Aldea M, Herrero E. 1997. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848.
12. Giaever G, Chu AM, Connell C, et al. 2002. Functional profiling of the Saccharomyces cerevisiae genome. Nature 418: 387-391.
13. Gietz RD, Schiestl RH. 1995. Transforming yeast with DNA. Methods Mol Cell Biol 5: 255-269.
14. Glickman MH, Rubin DM, Fried VA, Finley D. 1998. The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol 18: 3149-3162.
15. Goffeau A, Barrell BG, Bussey H, et al. 1996. Life with 6000 genes. Science 274: 546-567.
16. Harada H, Fujita T, Miyamoto M, et al. 1989. Structurally similar but functionally distinct factors, IRF-1 ad IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes. Cell 58: 729-739.
17. Hughes TR, Marton MJ, Jones AR, et al. 2000. Functional discovery via a compendium of expression profiles. Cell 102: 109-125.
18. Hughes TR. 2002. Yeast and drug discovery. Funct Integr Genom 2: 199-211.
19. International Human Genome Sequencing Consortium. 2004. Finishing the euchromatic sequence of the human genome. Nature 431: 935-941.
20. Lander ES, Linton LM, Birren B, et al. 2001. Initial sequencing and analysis of the human genome. Nature 409: 860-921.
21. Miyamoto M, Fujita T, Kimura Y, et al. 1988. Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-β gene regulatory elements. Cell 54: 903-913.
22. Mnaimneh A, Davierwala AP, Haynes J, et al. 2004. Exploration of essential gene functions via titratable promoter alleles. Cell 118: 31-44.
23. Oliver SG. 1996a. From DNA sequence to biological function. Nature 379: 597-600.
24. Oliver SG. 1996b. A network approach to the systematic analysis of yeast gene function. Trends Genet 12: 241-242.
25. Ross-Macdonald P, Coelho PS, Roemer T, et al. 1999. Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature 402: 413-418.
26. Rubin GM, Yandell MD, Wortman JR. et al. 2000. Comparative genomics of the eukaryotes. Science 287: 2204-2215.
27. Tuite MF, Brown AJP (eds). 1998. Methods in Microbiology. Yeast Gene Analysis. Academic Press: London; 473.
28. Venter CJ, Adams MD, Myers EW, et al. 2001. The sequence of the human genome. Science 291: 1304-1351.
29. Winzeler EA, Shoemaker DD, Astromoff A, et al. 1999. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285: 901-906.
30. Yen K, Gitsham P, Wishart J, Oliver SG, Zhang N. 2003. An improved tetO promoter replacement system for regulating expression of yeast genes. Yeast 20: 1255-1262.
31. Zhang N, Osborn M, Gitsham P, et al. 2003. Using yeast to place human genes in functional categories. Gene 303: 121-129.