Heterologous overexpression and purification of cytochrome c′ from Rhodobacter capsulatus and a mutant (K42E) in the dimerization region. Mutation does not alter oligomerization but impacts the heme iron spin state and nitric oxide binding properties

Biochemistry

Volumn 45, Issue 14, 2006, Pages 4388-4395

  Huston, Wilhelmina M ^a   Andrew, Colin R ^c   Servid, Amy E ^c   McKay, Alison L ^c   Leech, Andrew P ^a   Butler, Clive S ^b   Moir, James W B ^a  

^a UNIVERSITY OF YORK   (United Kingdom)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

^c EASTERN OREGON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABSORPTION; DIMERIZATION; DIMERS; IRON; RAMAN SPECTROSCOPY; RESONANCE; ULTRAVIOLET RADIATION;

FERROUS HEME-NITROSYL COMPLEX; RESONANCE RAMAN (RR) SPECTROSCOPY; RHODOBACTER CAPSULATUS CYTOCHROME C′ (RCCP);

PROTEINS;

CYTOCHROME C'; HEME; NITRIC OXIDE;

ARTICLE; DIMERIZATION; ELECTRON SPIN RESONANCE; ESCHERICHIA COLI; LIGAND BINDING; MUTATION; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RAMAN SPECTROMETRY; RHODOBACTER CAPSULATUS; ULTRACENTRIFUGATION; ULTRAVIOLET SPECTROSCOPY;

CARBON MONOXIDE; CYTOCHROMES C'; ELECTRON SPIN RESONANCE SPECTROSCOPY; HEME; IRON; MUTAGENESIS, SITE-DIRECTED; NITRIC OXIDE; PROTEIN STRUCTURE, QUATERNARY; RHODOBACTER CAPSULATUS; SPECTROPHOTOMETRY, ULTRAVIOLET; SPECTRUM ANALYSIS, RAMAN;

ESCHERICHIA COLI; RHODOBACTER CAPSULATUS;

EID: 33645668350     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi052605j     Document Type: Article

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