Studies on tropane alkaloid extraction by volatile organic solvents: Dichloromethane vs. chloroform

Phytochemical Analysis

Volumn 17, Issue 2, 2006, Pages 107-113

  Jaber Vazdekis, Nabil El ^a,b   Gutierrez Nicolás, Fátima ^a,b   Ravelo, Ángel G ^a,b   Zárate, Rafael ^a,b  

^a UNIVERSIDAD DE LA LAGUNA   (Spain)

^b HOSPITAL UNIVERSITARIO NUESTRA SEÑORA DE CANDELARIA   (Spain)

Author keywords

Atropa baetica; Chloroform; Dichloromethane; Solvent extraction; Tropane alkaloids

Indexed keywords

AMMONIA; BIOMOLECULES; GENE ENCODING; METABOLITES; ORGANIC SOLVENTS; SOLVENT EXTRACTION;

ATROPA BAETICUM; DRY WEIGHT; ENCODINGS; EXTRACTION METHOD; HAIRY ROOTS; HYOSCYAMINE-6Β-HYDROXYLASE; LIQUID MEDIA; TRANSGENICS; TROPANE ALKALOIDS; VOLATILE ORGANIC SOLVENTS;

DICHLOROMETHANE;

AMMONIA; ANISODAMINE; CHLOROFORM; DICHLOROMETHANE; HYOSCYAMINE; HYOSCYAMINE 6BETA HYDROXYLASE; METHANOL; OXYGENASE; SCOPOLAMINE; SULFURIC ACID; TROPANE ALKALOID; UNCLASSIFIED DRUG; VOLATILE ORGANIC COMPOUND;

ANALYTIC METHOD; ARTICLE; ATROPA; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTERMETHOD COMPARISON; METABOLITE; NONHUMAN; PLANT ROOT; SOLVENT EXTRACTION; STUDENT T TEST; TRANSGENIC PLANT;

ATROPA; ATROPA BAETICA;

EID: 33645567941     PISSN: 09580344     EISSN: 10991565     Source Type: Journal    
DOI: 10.1002/pca.893     Document Type: Article

References (23)

1. Agency for Toxic Substances and Disease Registry. 1998. Toxicological profile for methylene chloride (Update). Draft for public comment. Public Health Service, US Dept. of Health and Human Services: Atlanta, GA.
2. Bonhomme V, Laurain-Mattar D, Lacoux J, Fliniaux M, Jacquin-Dubreuil A. 2000. Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rol A, B, C genes only. J Biotechnol 81: 151-158.
3. Borris RP. 1996. Natural products research: Perspectives from a major pharmaceutical company. J Ethnopharmacot 51: 29-38.
4. Ditta G, Stanfield S, Gorbin D, Helinski DR. 1980. Broad host range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77: 7347-7351.
5. Dräger B. 2002. Analysis of tropane and related alkaloids. J Chromatogr A 978: 1-35.
6. European Community. 2000. Dangerous substances and preparations, marketing and regulations. Commission Directive no. 95/55/EC. European Community: Brussels.
7. Food and Drug Administration. 1976. Chloroform as an ingredient of human drug and cosmetic products. Fed. Reg. 41: 26842-26845. FDA: Maryland.
8. International Agency for Research on Cancer. 1979. Chloroform. In IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans, Vol. 20. Some Halogenated Hydrocarbons. World Health Organization: Lyon; 401-427.
9. Kamada H, Okamura N, Satake M, Harada H, Shimomura K. 1986. Alkaloid production by hairy root cultures of A. belladonna. Plant Cell Rep 5: 239-242.
10. Lee K.-T, Suzuki T, Yamakawa T, Kodama T, Igarashi, Y., Shimomura K. 1999. Production of tropane alkaloids by transformed root cultures of Atropa belladonna in stirred bioreactors with a stainless steel net. Plant Cell Rep 18: 567571.
11. Mano Y, Ohkawa H, Yamada Y. 1989. Production of tropane alkaloids by hairy root cultures of Duboisia leichhardtii transformed by Agrobacterium rhizogenes. Plant Sci 59: 191-201.
12. Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473-497.
13. Parr AJ, Robins RJ, Rhodes MJC. 1987. Release of secondary metabolites by plant-cell cultures. In Plant and Animal Cells: Process and Possibilities. Webb C, Mavituna F (ed.). Ellis Horwood: Chichester; 229-237.
14. Phillipson JD, Bisset NG. 1972. Quaternisation and oxidation of strychnine and brucine during plant extraction. Phytochemistry 11: 2547-2553.
15. Rocha P, Stenzel O, Parr A, Walton N, Christou P, Dräger B, Leech MJ. 2002. Functional expression of tropinone reductase I (tr-I) and hyoscyamine-6β-hydroxylase (h6h) from Hyoscyamus niger in Nicotiana tabacum. Plant Sci 162: 905-913.
16. Sandsborg WNAR. 1999. Natural products in drug discovery and development. Proc. Phytochem Soc Europe 43: 143-149.
17. Van Engelen FA, Molthoff JW, Conner AJ, Nap JP, Pereira A, Stiekema WJ. 1995. pBinPlus: An improved plant transformation vector based on pBin19. Transgenic Res 4: 288-290.
18. Vincze A, Gefen L. 1978. Solvent-cause quaternization as a possible source of error in the mass spectral quantitation of tertiary amines. I. Methylene chloride quaternization. Isr J Chem 17: 236-238.
19. Wink M. 1994. The cell culture medium - a functional extracellular compartment of suspension-cultured cells. Plant Cell Tissue Organ Cult 38: 307-319.
20. Zárate R. 1999. Tropane alkaloid production by Agrobacterium rhizogenes transformed hairy root cultures of Atropa baetica Willk. (Solanaceae). Plant Cell Rep 18: 418-423.
21. Zárate R, Hermosin B, Cantos M, Troncoso A. 1997a. Tropane alkaloid distribution in Atropa baetica plants. J Chem Ecol 23: 2059-2066.
22. Zárate R, Cantos M, Troncoso A. 1997b. Induction and development of adventitious shoots of Atropa baetica as a means of propagation. Euphytica 94: 361-366.
23. Zárate R, El Jaber-Vazdekis N, Medina B, Ravelo AG. 2005. Tailoring tropane alkaloid accumulation in transgenic Atropa baetica hairy roots by overexpressing the coding enzyme gene hyoscyamine 6β-hydroxylase (H6H) (submitted to Plant Cell Rep.)