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In previous reports,25,32 we used the signal at a fixed location outside the illuminated area to estimate SL [squares in Fig. 1(a)]. We found that this signal increased significantly with age for both A's (p<0.0001) but that it was also affected by the choroidal pigmentation (as estimated by iris color). Measurements showed that there was a gradient away from the image edge caused by the red AF diffusing in the choroid. We also found that the signal was smaller in the four study pseudophakic subjects compared with the age- and gender-matched phakic subjects (for A=470 nm, t=2.8, p=0.02).
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Crystalline lens AF was measured in three study subjects (ages 37, 46, and 57 years) by focusing the sampling volume within the lens. Excitation, normalized at 470 nm, were 0.91±0.01, 1.00±0.00, 0.53±0.02, and 0.24±0.02 at A=430, 470, 510, and 550 nm, respectively. Emission spectra were maximal at 520±5 nm for A=470 nm; at 600 nm, the fluorescence intensity increased with age of the three subjects. Excitation and emission spectra for one subject can be found in Fig. 8 of Ref. 74.
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Since DMP still decreased at 4° in some subjects (Figs. 1, 2, and 4), we are underestimating DMP'S compared with studies that used a reference at 7°. By exponential extrapolation of the profiles, we estimated that the DMP'S with a reference at 4° would be 0.02±0.03 D.U. (range 0.0 to -0.16 D.U.) less than those with the reference at 7°. Furthermore, our peak DMP'S were decreased by the smoothing that we used in our image analysis; reanalysis of our profiles using no smoothing caused the peak densities to increase by 0.03±0.02 D.U. (range 0.01-0.05 D.U.).
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