Real-time multiplex PCR assay to quantify hepatitis C virus RNA in peripheral blood mononuclear cells

Journal of Virological Methods

Volumn 133, Issue 2, 2006, Pages 195-204

  Pugnale, Paolo ^a   Latorre, Patrizia ^a   Rossi, Christine ^a   Crovatto, Katia ^a   Pazienza, Valerio ^a   De Gottardi, Andrea ^a   Negro, Francesco ^a  

^a Faculty of Medicine   (Switzerland)

Author keywords

Hepatitis C virus; Multiplex real time PCR; Peripheral blood mononuclear cell; qPCR

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; RNA;

ARTICLE; HEPATITIS C VIRUS; HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY;

5' UNTRANSLATED REGIONS; AMINO ACID SEQUENCE; CASE-CONTROL STUDIES; EVALUATION STUDIES; GENES, VIRAL; HEPACIVIRUS; HEPATITIS C; HUMANS; KINETICS; LEUKOCYTES, MONONUCLEAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; REAGENT KITS, DIAGNOSTIC; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY, AMINO ACID; TAQ POLYMERASE;

HEPATITIS C VIRUS;

EID: 33645313471     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2005.11.007     Document Type: Article

References (36)

1. C. Aberham, C. Pendl, P. Gross, G. Zerlauth, and M. Gessner A quantitative, internally controlled real-time PCR Assay for the detection of parvovirus B19 DNA J. Virol. Meth. 92 2001 183 191
2. T. Bizollon, S.N. Ahmed, S. Radenne, M. Chevallier, P. Chevallier, P. Parvaz, S. Guichard, C. Ducerf, J. Baulieux, F. Zoulim, and C. Trepo Long term histological improvement and clearance of intrahepatic hepatitis C virus RNA following sustained response to interferon-ribavirin combination therapy in liver transplanted patients with hepatitis C virus recurrence Gut 52 2003 283 287
3. J.M. Bland, and D.G. Altman Statistical methods for assessing agreement between two methods of clinical measurement Lancet 1 1986 307 310
4. J.P. Bronowicki, M.A. Loriot, V. Thiers, Y. Grignon, A.L. Zignego, and C. Brechot Hepatitis C virus persistence in human hematopoietic cells injected into SCID mice Hepatology 28 1998 211 218
5. S.A. Bustin Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays J. Mol. Endocrinol. 25 2000 169 193
6. J. Cervia, B. Kaplan, S. Schuval, and S. Weiss Virologic testing in the management of perinatal HIV exposure AIDS Read 13 2003 39 47
7. M.L. Collins, C. Zayati, J.J. Detmer, B. Daly, J.A. Kolberg, T.A. Cha, B.D. Irvine, J. Tucker, and M.S. Urdea Preparation and characterization of RNA standards for use in quantitative branched DNA hybridization assays Anal. Biochem. 226 1995 120 129
8. Consensus Panel National Institutes of Health Consensus Development Conference Statement: Management of hepatitis C: 2002 Hepatology 36 2002 S1 S252
9. D. Ducoulombier, A.M. Roque-Afonso, G. Di Liberto, F. Penin, R. Kara, Y. Richard, E. Dussaix, and C. Feray Frequent compartmentalization of hepatitis C virus variants in circulating B cells and monocytes Hepatology 39 2004 817 825
10. M.C. Edwards, and R.A. Gibbs Multiplex PCR: advantages, development, and applications PCR Meth. Appl. 3 1994 S65 S75
11. C. Feray, M. Gigou, D. Samuel, V. Paradis, J. Wilber, M.F. David, M. Urdea, M. Reynes, C. Brechot, and H. Bismuth The course of hepatitis C virus infection after liver transplantation Hepatology 20 1994 1137 1143
12. P. Ferenci Predicting the therapeutic response in patients with chronic hepatitis C: the role of viral kinetic studies J. Antimicrob. Chemother. 53 2004 15 18
13. M.W. Fried, M.L. Shiffman, K.R. Reddy, C. Smith, G. Marinos, F.L. Goncales Jr., D. Haussinger, M. Diago, G. Carosi, D. Dhumeaux, A. Craxi, A. Lin, J. Hoffman, and J. Yu Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection N. Engl. J. Med. 347 2002 975 982
14. W.G. Hodge, J.F. Boivin, S.H. Shapiro, R.G. Lalonde, K.C. Shah, B.D. Murphy, and F. Diaz-Mitoma Laboratory-based risk factors for cytomegalovirus retinitis Can. J. Ophthalmol. 39 2004 733 745
15. R.L. Hodinka The clinical utility of viral quantitation using molecular methods Clin. Diagn. Virol. 10 1998 25 47
16. M. Kondo, K. Tanaka, M. Ikeda, S. Arata, S. Saito, T. Sakaguchi, M. Morimoto, T. Fujii, K. Mitsui, H. Okazaki, M. Hoshino, and H. Sekihara Hepatic HCV-RNA as a predictor of outcome after interferon therapy in patients with chronic hepatitis C J. Gastroenterol. Hepatol. 11 1996 236 240
17. T. Laskus, M. Radkowski, L.F. Wang, H. Vargas, and J. Rakela Search for hepatitis C virus extrahepatic replication sites in patients with acquired immunodeficiency syndrome: specific detection of negative-strand viral RNA in various tissues Hepatology 28 1998 1398 1401
18. T. Laskus, M. Radkowski, A. Piasek, M. Nowicki, A. Horban, J. Cianciara, and J. Rakela Hepatitis C virus in lymphoid cells of patients coinfected with human immunodeficiency virus type 1: evidence of active replication in monocytes/macrophages and lymphocytes J. Infect. Dis. 181 2000 442 448
19. T. Laskus, M. Radkowski, A. Bednarska, J. Wilkinson, D. Adair, M. Nowicki, G.B. Nikolopoulou, H. Vargas, and J. Rakela Detection and analysis of hepatitis C virus sequences in cerebrospinal fluid J. Virol. 76 2002 10064 10068
20. J.E. Layden, T.J. Layden, K.R. Reddy, R.S. Levy-Drummer, J. Poulakos, and A.U. Neumann First phase viral kinetic parameters as predictors of treatment response and their influence on the second phase viral decline J. Viral. Hepat. 9 2002 340 345
21. T.J. Layden, J.E. Layden, R.M. Ribeiro, and A.S. Perelson Mathematical modeling of viral kinetics: a tool to understand and optimize therapy Clin. Liver Dis. 7 2003 163 178
22. H. Lerat, F. Berby, M.A. Trabaud, O. Vidalin, M. Major, C. Trepo, and G. Inchauspe Specific detection of hepatitis C virus minus strand RNA in hematopoietic cells J. Clin. Invest. 97 1996 845 851
23. K.J. Livak, S.J. Flood, J. Marmaro, W. Giusti, and K. Deetz Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization PCR Meth. Appl. 4 1995 357 362
24. M.P. Manns, J.G. McHutchison, S.C. Gordon, V.K. Rustgi, M. Shiffman, R. Reindollar, Z.D. Goodman, K. Koury, M. Ling, and J.K. Albrecht Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial Lancet 358 2001 958 965
25. F. Negro, K. Krawczynski, R. Quadri, L. Rubbia-Brandt, M. Mondelli, J.P. Zarski, and A. Hadengue Detection of genomic- and minus-strand of hepatitis C virus RNA in the liver of chronic hepatitis C patients by strand-specific semiquantitative reverse-transcriptase polymerase chain reaction Hepatology 29 1999 536 542
26. A.U. Neumann, N.P. Lam, H. Dahari, D.R. Gretch, T.E. Wiley, T.J. Layden, and A.S. Perelson Hepatitis C viral dynamics in vivo and the antiviral efficacy of interferon-alpha therapy Science 282 1998 103 107
27. C. Orlando, P. Pinzani, and M. Pazzagli Developments in quantitative PCR Clin. Chem. Lab. Med. 36 1998 255 269
28. S.D. Pas, E. Fries, R.A. De Man, A.D. Osterhaus, and H.G. Niesters Development of a quantitative real-time detection assay for hepatitis B virus DNA and comparison with two commercial assays J. Clin. Microbiol. 38 2000 2897 2901
29. M.W. Pfaffl A new mathematical model for relative quantification in real-time RT-PCR Nucl. Acids Res. 29 2001 e45
30. M. Radkowski, L.F. Wang, H.E. Vargas, J. Rakela, and T. Laskus Detection of hepatitis C virus replication in peripheral blood mononuclear cells after orthotopic liver transplantation Transplantation 66 1998 664 666
31. M.W. Russo, and M.W. Fried Guidelines for stopping therapy in chronic hepatitis C Curr. Gastroenterol. Rep. 6 2004 17 21
32. M. Schutten, and H.G. Niesters Clinical utility of viral quantification as a tool for disease monitoring Expert Rev. Mol. Diagn. 1 2001 153 162
33. M. Shindo, K. Arai, Y. Sokawa, and T. Okuno Hepatic hepatitis C virus RNA as a predictor of a long-term response to interferon-alpha therapy Ann. Intern. Med. 122 1995 586 591
34. H.I. Yang, U.H. Iloeje, J. Su, C.L. Jen, E. Kuo, S.L. You, and C.J. Chen Progression to decompensated cirrhosis in chronic hepatitis B virus infected persons is strongly associated with baseline viral load (abstract 538, 40th Ann. Meeting of the EASL) J. Hepatol. 42 Suppl. 2 2005 195
35. S. Zeuzem, J.M. Schmidt, J.H. Lee, B. Ruster, and W.K. Roth Effect of interferon alfa on the dynamics of hepatitis C virus turnover in vivo Hepatology 23 1996 366 371
36. S. Zeuzem, J.M. Schmidt, J.H. Lee, M. von Wagner, G. Teuber, and W.K. Roth Hepatitis C virus dynamics in vivo: effect of ribavirin and interferon alfa on viral turnover Hepatology 28 1998 245 252