Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis

Genesis (United States)

Volumn 44, Issue 2, 2006, Pages 66-74

  Goentoro, Lea A ^a,b   Yakoby, Nir ^a,b   Goodhouse, Joseph ^b   Schüpbach, Trudi ^b   Shvartsman, Stanislav Y ^a,b  

^a Princeton University   (United States)

^b PRINCETON UNIVERSITY   (United States)

Author keywords

Drosophila; EGFP; EGFR; GAL4 UAS; GFP; Quantitative fluorescence

Indexed keywords

GREEN FLUORESCENT PROTEIN; TRANSCRIPTION FACTOR GAL4;

ANIMAL EXPERIMENT; ARTICLE; CELL SPECIFICITY; CELL TYPE; CONTROLLED STUDY; DROSOPHILA; EMBRYO; EMBRYO DEVELOPMENT; GENE EXPRESSION; GENE FUNCTION; GENE TARGETING; GENETIC TRANSCRIPTION; MOUSE; NONHUMAN; OOCYTE DEVELOPMENT; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

ANIMALIA;

EID: 33645227329     PISSN: 1526954X     EISSN: 1526968X     Source Type: Journal    
DOI: 10.1002/gene.20184     Document Type: Article

References (22)

1. Alvarado D, Rice AH, Duffy JB. 2004. Bipartite inhibition of Drosophila epidermal growth factor receptor by the extracellular and transmembrane domains of Kekkon1. Genetics 107:187-202.
2. Brand AH, Perrimon N. 1993. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118:401-415.
3. Cormack BP, Valdivia RH, Falkow S. 1996. FACS-optimized mutant of the green fluorescent protein (GFP). Gene 173:33-38.
4. Dmochowski IJ, Dmochowski JE, Oliveri P, Davidson EH, Fraser SE. 2002. Quantitative imaging of cis-regulatory reporters in living embryos. Proc Natl Acad Sci U S A 99:12895-12900.
5. Duffy JB. 2001. GAL4 system in Drosohila: a fly geneticist's Swiss army knife. genesis 34:1-15.
6. Efron B, Tibshirani RJ. 1994. An introduction to bootstrap. Boca Raton, FL: CRC Press.
7. Forrest KM, Gavis ER. 2003. Live imaging of endogenous RNA reveals a diffusion and entrapment mechanism for nanos mRNA localization in Drosophila. Curr Biol 13:1150-1168.
8. Ghiglione C, Carraway KL 3rd, Amundadottir LT, Boswell RE, Perrimon N, Duffy JB. 1999. The transmembrane molecule kekkon 1 acts in a feedback loops to negatively regulate the activity of the Drosophila EGF receptor during oogenesis. Cell 96:847-856.
9. Halfon MS, Gisselbrecht S, Lu J, Estrada B, Keshishian H, Michelson AM. 2002. New fluorescent protein reporters for use with the Drosophila Gal4 expression system and for vital detection of balancer chromosomes. genesis 34:135-138.
10. Hartley KO, Nutt SL, Amaya E. 2002. Targeted gene expression in transgenic Xenopus using the binary GAL4-UAS system. Proc Natl Acad Sci U S A 99:1377-1382.
11. Hirschberg K, Miller CM, Ellenberg J, Presley JF, Siggia ED, Phair RD, Lippincott-Schwartz J. 1998. Kinetic analysis of secretory protein traffic and characterization of Golgi to plasma membrane transport intermediates in living cells. J Cell Biol 143:1485-1503.
12. Lippincott-Schwartz J, Patterson GH. 2003. Development and use of fluorescent protein markers in living cells. Science 300:87-91.
13. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402-408.
14. Ornitz DM, Moreadith RW, Leder P. 1991. Binary system for regulating transgene expression in mice: targeting int-2 gene expression with yeast GAL4/UAS control elements. Proc Natl Acad Sci U S A 88:698-702.
15. Pawley J. 2000. The 39 steps: a cautionary tale of quantitative 3-D fluorescence microscopy. BioTechniques 28:884-887.
16. Piston D, Patterson GH, Knobel SM. 1998. Quantitative imaging of the green fluorescent protein (GFP). In: Sullivan K, Kay S, editors. Methods in cell biology: green fluorescence proteins. San Diego: Academic Press. p 31-48.
17. Piston DW, Patterson H, Knobel SM. 1999. Quantitative imaging of the green fluorescent protein (GFP). Methods Cell Biol 58:31-48.
18. Queenan AM, Ghabrial A, Schupbach T. 1997. Ectopic activation of torpedo/Egfr, a Drosophila receptor tyrosine kinase dorsalizes both the eggshell and the embryo. Development 124:3871-3880.
19. Sandison DR, Williams RM, Wells KS, Strickler J, Webb WW. 1995. Quantitative fluorescence confocal laser scanning microscopy (CLSM). In: Pawley JB, editor. Handbook of biological confocal microscopy. New York: Plenum Press. p 39-53.
20. Scheer N, Campos-Ortega JA. 1999. Use of the GAL4-UAS technique for targeted gene expression in the zebrafish. Mech Dev 80:153-158.
21. Sen J, Goltz JS, Stevens L, Stein D. 1998. Spatially restricted expression of pipe in the Drosopbila egg chamber defines embryonic dorsal-ventral polarity. Cell 95:471-481.
22. Spradling AC. 1993. Developmental genetics of oogenesis. In: Bates M, Martinez AA, editors. The development of Drosopbila melanogaster. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. p 1-70.