Detection of activated platelets in canine blood by use of flow cytometry

American Journal of Veterinary Research

Volumn 67, Issue 1, 2006, Pages 56-63

  Wills, Tamara B ^a   Wardrop, K Jane ^b   Meyers, Ken M ^b  

^a IDEXX LABORATORIES   (United States)

^b WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; BIOLOGICAL MARKER; CD45 ANTIGEN; CD61 ANTIGEN; GLYCOPROTEIN IIIA; LIPOCORTIN 5; PADGEM PROTEIN; PHOSPHATIDYLSERINE; PROTHROMBIN; THROMBOCYTE ACTIVATING FACTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIGEN DETECTION; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; DIAGNOSTIC VALUE; DOG; FEMALE; FLOW CYTOMETRY; HEMOSTASIS; LEUKOCYTE AGGREGATION; MALE; NONHUMAN; PROTEIN EXPRESSION; THROMBOCYTE ACTIVATION; THROMBOCYTE AGGREGATION; THROMBOCYTE COUNT; THROMBOEMBOLISM; THROMBOGENESIS; VETERINARY MEDICINE;

ANALYSIS OF VARIANCE; ANIMALS; BIOLOGICAL MARKERS; BLOOD PLATELETS; DOGS; FLOW CYTOMETRY; LEUKOCYTES; P-SELECTIN; PHOSPHATIDYLSERINES; PLATELET ACTIVATION;

ANIMALIA; CANIS FAMILIARIS; THROMBUS;

EID: 33645217955     PISSN: 00029645     EISSN: None     Source Type: Journal    
DOI: 10.2460/ajvr.67.1.56     Document Type: Article

References (43)

1. Brooks M. Coagulopathies and thrombosis. In: Ettinger SJ, Feldman ED, eds. Texbook of veterinary internal medicine diseases of the dog and cat. 5th ed. Philadelphia: WB Saunders Co, 2000;1829-1841.
2. Klein MK, Dow SW, Rosychuk RAW. Pulmonary thromboembolism associated with immune-mediated hemolytic anemia in dogs: ten cases (1982-1987). J Am Vet Med Assoc 1989;195:246-250.
3. Littman MP, Dambach DM, Vaden SL, et al. Familial protein-losing enteropathy and protein-losing nephropathy in Soft Coated Wheaten Terriers: 222 cases (1983-1997). J Vet Intern Med 2000;14:68-80.
4. Green RA, Kabel AL. Hypercoagulable state in three dogs with nephrotic syndrome: role of acquired antithrombin III deficiency. J Am Vet Med Assoc 1982;181:914-917.
5. Collins CE, Cahill MR, Newland AC, et al. Platelets circulate in activated state in inflammatory bowel disease. Gastroenterology 1994;106:840-845.
6. Webberley MJ, Hart MT, Melikian V. Thromboembolism in inflammatory bowel disease: role of platelets. Gut 1993;34:247-251.
7. LaRue MJ, Murtaugh RJ. Pulmonary thromboembolism in dogs: 47 cases (1986-1987). J Am Vet Med Assoc 1990;197:1368-1372.
8. Dahlbäck B. Blood coagulation. Lancet 2000;355:1627-1632.
9. Brooks MB, Catalfamo JL, Brown HA, et al. A hereditary bleeding disorder of dogs caused by a lack of platelet procoagulant activity. Blood 2002;99:2434-2441.
10. Lentz BR. Exposure of platelet membrane phosphatidylserine regulates blood coagulation. Prog Lipid Res 2003;42:423-438.
11. Plow FE, Ginsberg MH. The molecular basis for platelet function. In: Hoffman R, Benz EJ, Shattil SJ, et al, eds. Hematology basic principles and practice. 3rd ed. New York: Churchill Livingstone Inc, 2000;1741-1766.
12. Marder VJ, Rosove MH, Minning DM. Foundation and sites of action of antithrombotic agents. Best Pract Res Clin Haematol 2004;17:3-22.
13. André P. P-selectin in haemostasis. Br J Haematol 2004;126:298-306.
14. Celi A, Pellegrini G, Lorenzet R, et al. P-selectin induces the expression of tissue factor on monocytes. Proc Natl Acad Sci U S A 1994;91:8767-8771.
15. Tocchetti EV, Flower RL, Lloyd JV. Assessment of in vitro-generated platelet microparticles using a modified flow cytometric strategy. Thromb Res 2001;103:47-55.
16. Wilkerson MJ, Shuman W. Alterations in normal canine platelets during storage in EDTA anticoagulated blood. Vet Clin Pathol 2001;30:107-113.
17. Wardrop KJ. Analysis of activated platelets in dogs - can we detect a prethrombotic state?, in Proceedings. 2002 Am Coll Vet Intern Med Forum 2002;630-631.
18. Michelson AD, Furman MI. Laboratory markers of platelet activation and their clinical significance. Curr Opin Hematol 1999;6:342-348.
19. Hagberg IA, Lyberg T. Evaluation of circulating platelet-leukocyte conjugates: a sensitive flow cytometric assay well suited for clinical studies. Platelets 2000;11:151-160.
20. Li N, Goodall AH, Hjemdahl P. Efficient flow cytometric assay for platelet-leukocyte aggregates in whole blood using fluorescence signal triggering. Cytometry 1999;35:154-161.
21. Nomura S. Function and clinical significance of platelet-derived mircroparticies. Int J Hematol 2001;74:397-404.
22. Michelson AD, Barnard MR, Krueger LA, et al. Circulating monocyte-platelet aggregates are a more sensitive marker of in vivo platelet activation than platelet surface P-selectin: studies in baboons, human coronary intervention, and human acute myocardial infarction. Circulation 2001;104:1533-1537.
23. Shattil SJ, Cunningham M, Hoxie JA. Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood 1987;70:307-315.
24. Li N, Goodall AH, Hjemdahl P. A sensitive flow cytometric assay for circulating platelet-leucocyte aggregates. Br J Haematol 1997;99:808-816.
25. Thiagarajan P, Tait JF. Binding of annexin V/placental anticoagulant protein 1 to platelets. Evidence for phosphatidylserine exposure in the procoagulant response of activated platelets. J Biol Chem 1990;265:17420-17423.
26. Glantz SA. Primer of biostatistics. 3rd ed. New York: McGraw-Hill Book Co, 1992;374.
27. Nelson LO, Andreasen C. The utility of plasma D-dimer to identify thromboembolic disease in dogs. J Vet Intern Med 2003;17:830-834.
28. Blockmans D, Deckmyn H, Vermylen J. Platelet activation. Blood Rev 1995;9:143-156.
29. Kurata M, Ishizuka N, Matsuzawa M, et al. A comparative study of whole-blood platelet aggregation in laboratory animals: its species differences and comparison with turbidimetric method. Comp Biochem Physiol 1995;112c:359-365.
30. Li N, Ilu I, Hjemdahl P. Aspirin treatment does not attenuate platelet or leukocyte activation as monitored by whole blood flow cytometry. Thromb Res 2003;111:165-170.
31. Kierzkowska B, Stanczyk J, Wiectawska B, et al. Activation of circulating platelets and platelet response to activating agents in children with cyanotic congenital heart disease: their relevance to palliative systemic-pulmonary shunt. Int J Cardiol 2001;79:49-59.
32. Nickels RM, Seyfert UT, Wenzel E, et al. A simple and reproducible method to reliably assess platelet activation. Thromb Res 2003;110:53-56.
33. Moritz A, Walcheck BK, Weiss DJ. Flow cytometric detection of activated platelets in the dog. Vet Clin Pathol 2003;32:6-12.
34. Moritz A, Walcheck BK, Weiss DJ. Evaluation of flow cytometric and automated methods for detection of activated platelets in dogs with inflammatory disease. Am J Vet Res 2005;66:325-329.
35. Lalko CC, Deppe E, Ulatowski D, et al. Equine platelet CD62P (P-selectin) expression: a phenotypic and morphologic study. Vet Immunol Immunopathol 2003;91:119-134.
36. Kugelmass AD, Shannon RP, Yeo EL, et al. Intravenous cocaine induces platelet activation in the conscious dog. Circulation 1995;91:1336-1340.
37. Leytin V, Mody M, Semple JW, et al. Quantification of platelet activation status by analyzing P-selectin expression. Biochem Biophys Res Commun 2000;273:565-570.
38. Rinder HM, Bonan JL, Rinder CS, et al. Activated and unactivated platelet adhesion to monocytes and neutrophils. Blood 1991;78:1760-1769.
39. Jensen MK, de Nully Bown P, Lund BV, et al. Increased circulating platelet-leukocyte aggregates in myeloproliferative disorders is correlated to previous thrombosis, platelet activation and platelet count. Eur J Haematol 2001;66:143-151.
40. Peters MJ, Heyderman RS, Hatch DJ, et al. Investigation of platelet-neutrophil interactions in whole blood by flow cytometry. J Immunol Methods 1997;209:125-135.
41. Tomer A. A sensitive and specific functional flow cytometric assay for the diagnosis of heparin-induced thrombocytopenia. Br J Haematol 1997;98:648-656.
42. Kingston JK, Bayly WM, Sellon DC, et al. Measurement of the activation of equine platelets by use of fluorescent-labeled annexin V, anti-human fibrinogen antibody, and anti-human thrombospondin antibody. Am J Vet Res 2002;63:513-519.
43. Michelson AD, Barnard MR, Hechtman HB, et al. In vivo tracking of platelets rapidly lose surface P-selectin but continue to circulate and function. Proc Natl Acad Sci U S A 1996;93:11877-11882.