Purification and characterization of a novel glucuronan lyase from Trichoderma sp. GL2

Applied Microbiology and Biotechnology

Volumn 70, Issue 4, 2006, Pages 437-443

  Delattre, C ^a   Michaud, P ^a   Keller, C ^a   Elboutachfaiti, R ^a   Beven, L ^b   Courtois, B ^a   Courtois, J ^a  

^a UNIVERSITÉ DE PICARDIE JULES VERNE   (France)

^b Université de Technologie de Compiègne   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON; ELECTROPHORESIS; ION EXCHANGE; MONOMERS; POLYSACCHARIDES; SIZE EXCLUSION CHROMATOGRAPHY;

ANION EXCHANGE CHROMATOGRAPHY; GLUCURONAN LYASE (GL); POLYSACCHARIDE LYASE;

FUNGI;

ACETIC ACID DERIVATIVE; ENDOPOLYGLUCURONATE LYASE; GLUCURONAN; GLUCURONAN LYASE; LYASE; MONOMER; POLYSACCHARIDE; POLYSACCHARIDE LYASE; ULVAN; UNCLASSIFIED DRUG;

FUNGUS;

ACETYLATION; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CARBON SOURCE; ELECTROPHORESIS; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SUBSTRATE; FUNGUS GROWTH; GEL PERMEATION CHROMATOGRAPHY; ISOELECTRIC POINT; MOLECULAR WEIGHT; NONHUMAN; TRICHODERMA;

MAGNETIC RESONANCE SPECTROSCOPY; POLYSACCHARIDE-LYASES; SUBSTRATE SPECIFICITY; TRICHODERMA;

FUNGI; TRICHODERMA;

EID: 33645214372     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0077-8     Document Type: Article

References (21)

1. Askew DJ, Laing MD (1993) An adapted selective medium for the quantitative isolation of Trichoderma species. Plant Pathol 42:686-690
2. Brimmer TA, Boland G (2003) A review of the non-target effects of fungi used to biologically control plant diseases. Agric Ecosyst Environ 100:3-16
3. Courtois J, Seguin JP, Declomesnil S, Heyraud A, Colin-Morel P, Dantas L, Barbotin JN, Courtois B (1993) A (1→4)-β-D-glucuronan excreted by a mutant of Rhizobium meliloti M5N1 strain. J Carbohydr Chem 12:441-448
4. Da Costa A, Michaud P, Petit E, Heyraud A, Colin-Morel P, Courtois B, Courtois J (2001) Purification and properties of a glucuronan lyase from Sinorhizobium meliloti M5N1CS (NCIMB 40472). Appl Environ Microbiol 67:5197-5203
5. Da Costa A, Michaud P, Heyraud A, Colin-Morel P, Courtois B, Courtois J (2003) Acetyl substitution of glucuronan influences glucuronan cleavage by glya from Sinorhizobium meliloti M5N1CS (NCIMB 40472). Carbohydr Polym 51:223-228
6. Dantas L, Courtois J, Courtois B, Séguin JP, Gey C, Heyraud A (1994) NMR spectroscopic investigation of oligoglucuronates prepared by enzymatic hydrolysis of a (1→4)-β-D-glucuronan. Carbohydr Res 265:303-310
7. Deane EE, Whipps JM, Lynch JM, Peberdy JF (1998) The purification and characterization of a Trichoderma harzanium exochitinase. Biochim Biophys Acta 1383:101-110
8. De la Cruz J, Pintor-Toro JA, Benitez T, Llobell A, Romero LC (1995) A novel endo-β-1,3-glucanase, BGN13.1 involved in the mycoparasitism of Trichoderma harzanium. J Bacteriol 177 (23):6937-6945
9. De Ruiter GA, Josso SL, Colquhoun IJ, Voragen AGJ, Rombouts FM (1992) Isolation and characterization of β(1-4)-D-glucuronans from extracellular polysaccharides of moulds belonging to Mucorales. Carbohydr Polym 18:1-7
10. Harman GE, Howell CR, Viterbo A, Chet I, Lorito M (2004) Trichoderma species - opportunistic, avirulent plant symbionts. Nat Rev Microbiol 2:43-56
11. Hjeljord L, Tronsmo A (1998) Trichoderma and Gliocladium in biological control: an overview. In: Harman GE, Kubicek C (eds) Trichoderma and Gliocladium: enzymes, biological control and commercial applications, vol 2. Taylor and Francis, London, UK, pp 131-151
12. Kubicek CP (1992) The cellulase proteins of Trichoderma reesei: structure, multiplicity, mode of action and regulation of formation. Adv Biochem Eng Biotechnol 45:1-27
13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
14. Lahaye M (1998) NMR spectroscopic characterisation of oligosaccharides from two Ulva rigida ulvan samples (Ulvales, Chlorophyta) degraded by a lyase. Carbohydr Res 314:1-12
15. Lahaye M, Brunel M, Bonnin E (1997) Fine chemical structure analysis of oligosaccharides produced by an ulvan-lyase degradation of the water-soluble cell-wall polysaccharides from Ulva sp. (Ulvales, Chlorophyta). Carbohydr Res 304:325-333
16. 2+ on production and O-acetylation of glucuronan excreted by the Rhizobium meliloti M5N1CS strain during fermentation. Lett Appl Microbiol 20:110-112
17. Michaud P, Da Costa A, Courtois B, Courtois J (2003) Polysaccharide lyases - recent development as biotechnological tools. Crit Rev Biotechnol 23(4):233-266
18. Pecina A, Paneque A (1994) Detection of alginate lyase by activity staining after SDS PAGE and subsequent renaturation. Anal Biochem 217:124-127
19. Ray B, Lahaye M (1995) Cell-wall polysaccharides from the marine green algae Ulva "rigida" (ulvales, chlorophyta). Extraction and chemical composition. Carbohydr Res 274:251-261
20. Schoffelmeer EAM, Klis FM, Sietsma JH, Cornelissen BJC (1999) The cell wall of Fusarium oxysporum. Fungal Genet Biol 27:275-282
21. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MDE, Fujimoto K, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76-85