Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation

Molecular Microbiology

Volumn 59, Issue 1, 2006, Pages 288-300

  Vedantam, Gayatri ^a   Knopf, Sarah ^a   Hecht, David W ^a  

^a LOYOLA UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; CYTOSINE; DINUCLEOTIDE; GUANINE; PROTEIN BMPH; UNCLASSIFIED DRUG;

ARTICLE; BACTEROIDES FRAGILIS; BASE PAIRING; COMPLEX FORMATION; CONJUGATION; CONTROLLED STUDY; DNA SYNTHESIS; ENZYME SUBSTRATE COMPLEX; GEL MOBILITY SHIFT ASSAY; GENE CONSTRUCT; GENE FUNCTION; GENE MUTATION; GENE SEQUENCE; GRAM POSITIVE BACTERIUM; INVERTED REPEAT; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID VECTOR; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PURIFICATION; PROTEIN TRANSPORT; TRANSPOSON;

BACTERIAL PROTEINS; BACTEROIDES FRAGILIS; BASE PAIRING; BASE SEQUENCE; CONJUGATION, GENETIC; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; NUCLEIC ACID CONFORMATION; REPLICATION ORIGIN;

BACTERIA (MICROORGANISMS); BACTEROIDES FRAGILIS; BACTEROIDES SP.; ESCHERICHIA COLI; POSIBACTERIA;

EID: 33645077344     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2005.04934.x     Document Type: Article

References (65)

1. Ausubel FM. Brent R. Kingston RE. Moore DD. Seidman JG. Smith JA. Struhl K. 1998 Current Protocols in Molecular Biology New York John Wiley and Sons
2. Bass KA. Hecht DW. 2002 Isolation and characterization of cLV25, a Bacteroides fragilis chromosomal transfer factor resembling multiple Bacteroides sp. mobilizable transposons J Bacteriol 184 1895 1904
3. Becker EC. Meyer RJ. 2001 Recognition of oriT for DNA processing at termination of a round of conjugal transfer J Mol Biol 300 1067 1077
4. Bonheyo G. Graham D. Shoemaker NB. Salyers AA. 2001 Transfer region of a Bacteroides conjugative transposon, CTnDOT Plasmid 45 41 51
5. Bonnefoy E. 1997 The ribosomal S16 protein of Escherichia coli displaying a DNA-nicking activity binds to cruciform DNA Eur J Biochem 247 852 859
6. Bonnefoy E. Takahashi M. Yaniv JR. 1994 DNA-binding parameters of the HU protein of Escherichia coli to cruciform DNA J Mol Biol 242 116 129
7. Brasch MA. Meyer RJ. 1987 A 38 base-pair segment of DNA is required in cis for conjugative mobilization of broad host-range plasmid R1162 J Mol Biol 198 361 369
8. Brook I. 1989 Anaerobic bacterial bacteremia: 12-year experience in two military hospitals J Infect Dis 160 1071 1075
9. Byrd DR. Matson SW. 1997 Nicking by transesterification: the reaction catalysed by a relaxase Mol Microbiol 25 1011 1022
10. Caryl JA. Smith MC. Thomas CD. 2004 Reconstitution of a staphylococcal plasmid-protein relaxation complex in vitro J Bacteriol 186 3374 3383
11. Clarke P. Cuiv PO. O'Connell M. 2005 Novel mobilizable prokaryotic two-hybrid system vectors for high-throughput protein interaction mapping in Escherichia coli by bacterial conjugation Nucleic Acids Res 33 e18
12. Coupland GM. Brown AM. Willetts NS. 1987 The origin of transfer (oriT) of the conjugative plasmid R46: characterization by deletion analysis and DNA sequencing Mol Gen Genet 208 219 225
13. Covarrubias L. Cervantes L. Covarrubias A. Soberon X. Vichido I. Blanco A. et al. 1981 Construction and characterization of new cloning vehicles. V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328 Gene 13 25 35
14. Crellin PK. Rood JI. 1998 Tn4551 from Clostridium perfringens is a mobilizable transposon that encodes the functional Mob protein, TnpZ Mol Microbiol 27 631 642
15. Drolet M. Zanga P. Lau PC. 1990 The mobilization and origin of transfer regions of a Thiobacillus ferrooxidans plasmid: relatedness to plasmids RSF1010 and pSC101 Mol Microbiol 4 1381 1391
16. Ducote MJ. Prakash S. Pettis GS. 2000 Minimal and contributing sequence determinants of the cis-acting locus of transfer (clt) of streptomycete plasmid pIJ101 occur within an intrinsically curved plasmid region J Bacteriol 182 6834 6841
17. Furste JP. Pansegrau W. Ziegelin G. Kroger M. Lanka E. 1989 Conjugative transfer of promiscuous IncP plasmids: interaction of plasmid-encoded products with the transfer origin Proc Natl Acad Sci USA 86 1771 1775
18. Furuya N. Komano T. 1997 Mutational analysis of the R64 oriT region: requirement for precise location of the NikA-binding sequence J Bacteriol 179 7291 7297
19. Furuya N. Komano T. 2000 Initiation and termination of DNA transfer during conjugation of IncI1 plasmid R64: roles of two sets of inverted repeat sequences within oriT in termination of R64 transfer J Bacteriol 182 3191 3196
20. Goodrich JA. Schwartz ML. McClure WR. 1990 Searching for and predicting the activity of sites for DNA binding proteins: compilation and analysis of the binding sites for Escherichia coli integration host factor (IHF) Nucleic Acids Res 18 4993 5000
21. Guasch A. Lucas M. Moncalian G. Cabezas M. Perez-Luque R. Gomis-Ruth FX. et al. 2003 Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC Nat Struct Biol 10 1002 1010
22. Guiney DG. Lanka E. 1989 Conjugative transfer of IncP plasmids. In Promiscuous Plasmids of Gram Negative Bacteria Thomas CM. (ed.). London Academic Press Ltd, pp. 27 56
23. Guiney DG. Deiss C. Simnad V. 1988 Location of the relaxation complex nick site within the minimal origin of transfer region of RK2 Plasmid 20 259 265
24. Guzman LM. Espinosa M. 1997 The mobilization protein, MobM, of the streptococcal plasmid pMV158 specifically cleaves supercoiled DNA at the plasmid oriT J Mol Biol 266 688 702
25. Hecht DW. 2004 Prevalence of antibiotic resistance in anaerobic bacteria: worrisome developments Clin Infect Dis 39 92 97
26. Helke A. Geisen RM. Vollmer M. Sprengart ML. Fuchs E. 1993 An unstructured mRNA region and a 5′ hairpin represent important elements of the E. coli translation initiation signal determined by using the bacteriophage T7 gene 1 translation start site Nucleic Acids Res 21 5705 5711
27. Inamoto S. Fukuda H. Tatsuhiko A. Ohtsubo E. 1994 Site- and strand-specific nicking at oriT of plasmid R100 in a purified system: enhancement of the nicking activity of TraI (helicase I) with TraY and IHF J Biochem 116 838 844
28. Khan SA. 2000 Plasmid rolling-circle replication: recent developments Mol Microbiol 37 477 484
29. Lanka E. Wilkins BM. 1995 DNA processing reactions in bacterial conjugation Ann Rev Biochem 64 141 169
30. Li LY. Shoemaker NB. Wang GR. Cole SP. Hashimoto MK. Wang J. Salyers AA. 1995 The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette J Bacteriol 177 3940 3945
31. Llosa M. Grandoso G. de la CF. 1993 Nicking activity of TrwC directed against the origin of transfer of the IncW plasmid R388 J Mol Biol 246 54 62
32. Murphy CG. Malamy MH. 1993 Characterization of a 'mobilization cassette' in transposon Tn4399 from Bacteroides fragilis JBacteriol 175 5814 5823
33. Murphy CG. Malamy MH. 1995 Requirements for strand- and site-specific cleavage within the oriT region of Tn4399, a mobilizing transposon from Bacteroides fragilis J Bacteriol 177 3158 3165
34. Nguyen MH. Yu VL. Morris AJ. McDermott L. Wagener MW. Harrell L. Snydman DR. 2000 Antimicrobial resistance and clinical outcome of Bacteroides bacteremia: findings of a multicenter prospective observational trial Clin Infect Dis 30 870 876
35. Novicki TJ. Hecht DW. 1995 Characterization and DNA sequence of the mobilization region of pLV22a from Bacteroides fragilis J Bacteriol 177 4466 4473
36. Osborn AM. Boltner D. 2002 When phage, plasmids, and transposons collide: genomic islands, and conjugative- and mobilizable-transposons as a mosaic continuum Plasmid 48 202 212
37. Page MI. 1974 Beware - the anaerobe bandwagon N Engl J Medical 290 338 340
38. Pan X. 2004 Determination of the reopening temperature of a DNA hairpin structure in vitro Eur J Biochem 271 3665 3670
39. Pansegrau W. Lanka E. 1991 Common sequence motifs in DNA relaxases and nick regions from a variety of DNA transfer systems Nucleic Acids Res 19 3455
40. Pansegrau W. Lanka E. 1996a Enzymology of DNA transfer by conjugative mechanisms Prog Nucl Acid Res Mol Biol 54 197 251
41. Pansegrau W. Lanka E. 1996b Mechanisms of initiation and termination reactions in conjugative DNA processing J Biol Chem 271 13068 13076
42. Pinson V. Takahashi M. Rouviere-Yaniv J. 1999 Differential binding of the Escherichia coli HU, homodimeric forms and heterodimeric form to linear, gapped and cruciform DNA J Mol Biol 287 485 497
43. Rose RE. 1988 The nucleotide sequence of pACYC184 Nucleic Acids Res 16 355
44. Salyers AA. Shoemaker NB. 1997 Resistance gene transfer in anaerobes: new insights, new problems Clin Infect Dis 23 S36 S43
45. Salyers AA. Gupta A. Wang Y. 2004 Human intestinal bacteria as reservoirs for antibiotic resistance genes Trends Microbiol 12 412 416
46. Scherzinger E. Lurz R. Otto S. Dobrinski B. 1992 In vitro cleavage of double- and single-stranded DNA by plasmid RSF1010-encoded mobilization proteins Nucleic Acids Res 20 41 48
47. Scherzinger E. Kruft V. Otto S. 1993 Purification of the large mobilization protein of plasmid RSF1010 and characterization of its site-specific DNA - cleaving/DNA-joining activity Eur J Biochem 217 929 938
48. Scott KP. 2002 The role of conjugative transposons in spreading antibiotic resistance between bacteria that inhabit the gastrointestinal tract Cell Mol Life Sci 59 2071 2082
49. Shiflett PR. Taylor-McCabe KJ. Michalczyk R. Silks LA. Gupta G. 2003 Structural studies on the hairpins at the 3′ untranslated region of an anthrax toxin gene Biochemistry 42 6078 6089
50. Shoemaker NB. Vlamakis H. Hayes K. Salyers AA. 2001 Evidence for extensive resistance gene transfer among Bacteroides spp and among Bacteroides and other genera in the human colon Appl Environ Microbiol 67 561 568
51. Sitailo LA. Zagariya AM. Arnold PJ. Vedantam G. Hecht DW. 1998 The Bacteroides fragilis BtgA mobilization protein binds to the oriT region of pBFTM10 J Bacteriol 180 4922 4928
52. Smith CJ. Parker AC. 1998 The transfer origin for Bacteroides mobilizable transposon Tn4555 is related to a plasmid family from gram-positive bacteria J Bacteriol 180 435 439
53. Smith CJ. Tribble GD. Bayley DP. 1998 Genetic elements of Bacteroides species: a moving story Plasmid 40 12 29
54. Szpirer CY. Faelen M. Couturier M. 2001 Mobilization function of the pBHR1 plasmid, a derivative of the broad-host-range plasmid pBBR1 J Bacteriol 183 2101 2110
55. Thadepalli H. Gorbach SL. Broido PW. Norsen J. Nyhus L. 1973 Abdominal trauma, anaerobes, and antibiotics Surg Gynecol Obstet 137 270 276
56. Trinh S. Reysset G. 1997 Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis J Bacteriol 179 4071 4074
57. Vedantam G. Hecht DW. 2002 Isolation and characterization of BTF-37: chromosomal DNA captured from Bacteroides fragilis that confers self-transferability and expresses a pilus-like structure in Bacteroides spp and Escherichia coli J Bacteriol 184 728 738
58. Vedantam G. Novicki TJ. Hecht DW. 1999 Bacteroides fragilis transfer factor Tn5520: the smallest bacterial mobilizable transposon containing single integrase and mobilization genes that function in Escherichia coli J Bacteriol 181 2564 2571
59. Wang A. Macrina FL. 1995 Streptococcal plasmid pIP501 has a functional oriT site J Bacteriol 177 4199 4206
60. Waters VL. 1999 Conjugative transfer in the dissemination of beta-lactam and aminoglycoside resistance Front Biosci 4 D433 D456
61. Waters VL. Guiney DG. 1993 Processes at the nick region link conjugation, T-DNA transfer and rolling circle replication Mol Microbiol 9 1123 1130
62. Whittle G. Shoemaker NB. Salyers AA. 2002 The role of Bacteroides conjugative transposons in the dissemination of antibiotic resistance genes Cell Mol Life Sci 59 2044 2054
63. Wilkins B. Lanka E. 1993 DNA processing and replication during plasmid transfer between gram-negative bacteria. In Bacterial Conjugation Clewell DB. (ed.). New York Plenum Press, pp. 105 136
64. Ziegelin G. Pansegrau W. Lurz R. Lanka E. 1992 TraK protein of conjugative plasmid RP4 forms a specialized nucleoprotein complex with the transfer origin J Biol Chem 267 17279 17286
65. Zuker M. 2003 Mfold web server for nucleic acid folding and hybridization prediction Nucleic Acids Res 31 3406 3415