Evaluation of low density array technology for quantitative parallel measurement of multiple genes in human tissue

BMC Genomics

Volumn 7, Issue , 2006, Pages

  Goulter, Andrew B ^a   Harmer, Daniel W ^a   Clark, Kenneth L ^a  

^a Pharmagene Laboratories Ltd   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; INTERLEUKIN 8; MONOCYTE CHEMOTACTIC PROTEIN 1; RANTES; MESSENGER RNA; PRIMER DNA; THROMBIN;

ACCURACY; ARTICLE; BIOTECHNOLOGY; CONTROLLED STUDY; CORRELATION COEFFICIENT; ENDOTHELIUM CELL; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; HUMAN TISSUE; NUCLEOTIDE SEQUENCE; PROTEIN LOCALIZATION; PROTEIN MICROARRAY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; TISSUE DISTRIBUTION; COMPARATIVE STUDY; CYTOLOGY; DNA MICROARRAY; DRUG EFFECT; EVALUATION; METABOLISM; METHODOLOGY; REPRODUCIBILITY; VASCULAR ENDOTHELIUM;

DNA PRIMERS; ENDOTHELIUM, VASCULAR; GENE EXPRESSION PROFILING; HUMANS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; THROMBIN;

EID: 33645030115     PISSN: 14712164     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/1471-2164-7-34     Document Type: Article

References (8)

1. Ding C, Cantor CR: Quantitative analysis of nucleic acids - the last few years of progress. J Biochem Mol Biol 2004, 37:1-10.
2. Stanton LW: Methods to Profile Gene Expression. Trends Cardiovasc Med 2001, 11:49-54.
3. Abruzzo LV, Lee KY, Fuller A, Silverman A, Keating MJ, Medeiros LJ, Coombes KR: Validation of oligonucleotide microarray data using microfluidic low-density arrays: a new statistical method to normalize real-time RT-PCR data. Biotechniques 2005, 38:785-92.
4. Rus HG, Vlaicu R, Niculescu F: Interleukin-6 and interleukin-8 protein and gene expression in human arterial atherosclerotic wall. Atherosclerosis 1996, 127:263-71.
5. Liu Y, Hulten LM, Wiklund O: Macrophages isolated from human atherosclerotic plaques produce IL-8, and oxysterols may have a regulatory function for IL-8 production. Arterioscler Thromb Vasc Biol 1997, 17:317-23.
6. Briones MA, Phillips DJ, Renshaw MA, Hooper WC: Expression of chemokine by human coronary-artery and umbilical-vein endothelial cells and its regulation by inflammatory cytokines. Coron Artery Dis 2001, 12:179-86.
7. Haller F, Kulle B, Schwager S, Gunawan B, von Heydebreck A, Sultmann H, Fuzesi L: Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference genes suitable for normalization. Anal Biochem 2004, 335:1-9.
8. Bowen WP, Carey JE, Miah A, McMurray HF, Munday PW, James RS, Coleman RA, Brown AM: Measurement of cytochrome P450 gene induction in human hepatocytes using quantitative real-time reverse transcriptase-polymerase chain reaction. Drug Metab Dispos 2000, 28:781-87.