Determination of free tetrahydrocortisol and tetrahydrocortisone ratio in urine by liquid chromatography-tandem mass spectrometry

Scandinavian Journal of Clinical and Laboratory Investigation

Volumn 66, Issue 2, 2006, Pages 147-160

  Turpeinen, U ^a   Markkanen, H ^a   Sane, T ^b   Hamalainen E ^a  

^a HELSINKI UNIVERSITY CENTRAL HOSPITAL   (Finland)

^b Department of Endocrinology ^*  (Finland)

Author keywords

Apparent mineralocorticoid excess (AME); Electrospray ionization mass spectrometry; LC MS MS; Solid phase extraction (SPE)

Indexed keywords

ACETIC ACID ETHYL ESTER; ALLOTETRAHYDROCORTISOL; STEROID; TETRAHYDROCORTISOL; TETRAHYDROCORTISONE; UNCLASSIFIED DRUG;

ACCURACY; ANALYTIC METHOD; ARTICLE; CORRELATION ANALYSIS; GAS CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTERMETHOD COMPARISON; OBSERVER VARIATION; PRIORITY JOURNAL; REPRODUCIBILITY; SIGNAL NOISE RATIO; SOLID PHASE EXTRACTION; TANDEM MASS SPECTROMETRY; URINALYSIS; URINE LEVEL; VALIDATION PROCESS;

GLYCYRRHIZA;

EID: 33645013872     PISSN: 00365513     EISSN: 15027686     Source Type: Journal    
DOI: 10.1080/00365510500474504     Document Type: Article

References (17)

1. Best R, Walker BR. Additional value of measurement of urinary cortisone and unconjugated cortisol metabolites in assessing the activity of 11 β-hydroxysteroid dehydrogenase in vivo. Clin Endocrinol 1997;47:231-6.
2. Stewart PM, Wallace AM, Valentino R, Burt D, Shackleton CHL, Edwards CRW. Mineralocorticoid activity of liquorice: 11-beta-hydroxysteroid dehydrogenase deficiency comes of age. Lancet 1987;10:821-4.
3. Naray-Fejes-Toth A, Rusvai E, Fejes-Toth G. Is the renal type III corticosteroid-binding site the collecting duct-specific isoform of 11β-hydroxysteroid dehydrogenase? Endocrinology 1994;134:1671-5.
4. Shackleton CHL. Profiling steroid hormones and urinary steroids. J Chrom 1986;379:91-56.
5. Palermo M, Schackleton CHL, Mantero F, Stewart PM. Urinary free cortisone and the assessment of 11 β-hydroxysteroid dehydrogenase activity in man. Clin Endocrinol 1996;45:605-11.
6. Mantero F, Palermo M, Petrelli MD, Tedde R, Stewart PM, Schackleton CHL. Apparent mineralocorticoid excess: type I and type II. Steroids 1996;61:193-6.
7. Palermo M, Gomez-Sanchez C, Roitman E, Shackleton CHL. Quantitation of cortisol and related 3-oxo-4-ene steroids in urine using gas chromatography/mass spectrometry with stable isotope-labeled internal standards. Steroids 1996;61:583-9.
8. Hämäläinen E, Fotsis T, Adlercreutz H. A gas chromatographic method for the determination of neutral steroid profiles in urine, including studies on the effect of oxytetracycline administration on these profiles in men. Clin Chim Acta 1991;199:205-20.
9. Passing H, Bablok W. A new biometrical procedure for testing the equality of measurements from two different analytical methods. J Clin Chem Biochem 1983;21:709-20.
10. Wilson RC, Krozowski ZS, Li K, Obeyesekere VR, Razzaghy-Azar M, Harbison MD, et al. A mutation in the HSD11B2 gene in a family with apparent mineralocorticoid excess. J Clin Endocrinol Metab 1995;80:2263-6.
11. Shackleton CHL. Mass spectrometry in the diagnosis of steroid-related disorders and in hypertension research. J Steroid Biochem Molecular Biol 1993;45:127-40.
12. Dodds HM, Taylor PJ, Cannell GR, Pond SM. A high-performance liquid chromatography-electrospray-tandem mass spectrometry analysis of cortisol and metabolites in placental perfusate. Anal Biochem 1997;247:342-7.
13. Kasuya Y, Shibasaki H, Furuta T. The use of deuterium-labeled cortisol for in vivo evaluation of renal 11β-HSD activity in man: urinary excretion of cortisol, cortisone and their A-ring reduced metabolites. Steroids 2000;65:89-97.
14. Choi MH, Hahm JR, Jung BH, Chung BC. Measurement of corticoids in the patients with clinical features indicative of mineralocorticoid excess. Clin Chim Acta 2002;320:95-9.
15. Shibasaki H, Tanabe C, Furuta T, Kasuya Y. Hydrolysis of conjugated steroids by the combined use of β-glucuronidase preparations from Helix pomatia and Ampullaria: determination of urinary cortisol and its metabolites. Steroids 2001;66:795-801.
16. Weykamp CW, Penders TJ, Schmidt NA, Borburgh AJ, van de Calseyde JF, Wolthers BJ. Steroid profile for urine: reference values. Clin Chem 1989;35:2281-4.
17. Wilson RC, Nimkarn S, New MI. Apparent mineralocorticoid excess. Trends Endocrinol Metab 2001;12:104-11.