Concurrent mRNA and protein extraction from the same experimental sample using a commercially available column-based RNA preparation kit

BioTechniques

Volumn 40, Issue 1, 2006, Pages 54-58

  Morse, Silas M J ^a   Shaw, Gerry ^a   Larner, Stephen F ^a,b  

^a UNIVERSITY OF FLORIDA   (United States)

^b UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; PROTEIN;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; IMMUNOBLOTTING; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN ISOLATION; RAT; RNA EXTRACTION; RNA ISOLATION; SAMPLE; WESTERN BLOTTING; ANIMAL; BRAIN LEVEL; CELL CULTURE; COMPARATIVE STUDY; EVALUATION; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METHODOLOGY;

ANIMALS; BRAIN CHEMISTRY; CELLS, CULTURED; CHROMATOGRAPHY, LIQUID; PROTEINS; RATS; RNA, MESSENGER;

EID: 33644965802     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112100     Document Type: Article

References (3)

1. Ilaria, R., D. Wines, S. Pardue, S. Jamison, S.R. Ojeda, J. Snider, and M.R. Morrison. 1985. A rapid microprocedure for isolating RNA from multiple samples of human and rat brain. J. Neurosci. Methods 15:165-174.
2. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
3. Chomczynski, P. 1993. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques 15:532-537.