The attachment of V79 and human periodontal ligament fibroblasts on periodontally involved root surfaces following treatment with EDTA, citric acid, or tetracycline HCL: An SEM in vitro study

Journal of Contemporary Dental Practice

Volumn 7, Issue 1, 2006, Pages 044-059

  Chandra, R Viswa ^a   Jagotia, Ganesh Chandra ^b   Bhat, K Mahalinga ^a  

^a India   (India)

^b KASTURBA MEDICAL COLLEGE   (India)

Author keywords

Citric acid; EDTA; HPDL; Human periodontal ligament fibroblasts; Periodontal diseases; Root conditioning; Tetracycline HCl; V79 cells

Indexed keywords

CITRIC ACID; EDETIC ACID; GENTAMICIN; GLUTARALDEHYDE; PHOSPHATE BUFFERED SALINE; TETRACYCLINE; WATER; ANTIINFECTIVE AGENT; CHELATING AGENT;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL MIGRATION; CELL STRUCTURE; CLINICAL ARTICLE; COMPARATIVE STUDY; DISTILLATION; DRUG EFFECT; FIBROBLAST CULTURE; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; LUNG FIBROBLAST; NONHUMAN; PERIODONTAL LIGAMENT; PERIODONTICS; PERIODONTITIS; SCANNING ELECTRON MICROSCOPY; TISSUE FIXATION; TISSUE REGENERATION; TISSUE REPAIR; TOOTH CROWN; TOOTH ROOT; ANIMAL; CELL LINE; CELL PROLIFERATION; CRICETULUS; CYTOLOGY; DENTAL ETCHING; DENTAL SURGERY; FIBROBLAST; HAMSTER; PATHOPHYSIOLOGY; REGENERATION; SURFACE PROPERTY;

ANIMALS; ANTI-BACTERIAL AGENTS; CELL ADHESION; CELL LINE, TRANSFORMED; CELL PROLIFERATION; CHELATING AGENTS; CITRIC ACID; CRICETINAE; CRICETULUS; DENTAL ETCHING; EDETIC ACID; FIBROBLASTS; HUMANS; MICROSCOPY, ELECTRON, SCANNING; PERIODONTAL LIGAMENT; PERIODONTITIS; REGENERATION; SMEAR LAYER; SURFACE PROPERTIES; TETRACYCLINE; TOOTH ROOT;

EID: 33644930285     PISSN: 15263711     EISSN: 15263711     Source Type: Journal    
DOI: 10.5005/jcdp-7-1-35     Document Type: Article

References (34)

1. Babay N. The effect of EDTA on the attachment and growth of cultured human gingival fibroblasts in periodontitis-affected root surface. J Contemp Dent Pract 2001; 15;2: 13-23.
2. Blomlöf J, Lindskog, S. Root surface texture and early cell and tissue colonization after different etching modalities. Eur J Oral Sci 1995; 103: 17-24.
3. Isik G, Ince S, Saglam F, et al. Comparative SEM study on the effect of different demineralization methods with tetracycline HCl on healthy root surfaces. J Clin Periodontol 1997; 24(9 Pt 1): 589-94.
4. Babay N. Attachment of human gingival fibroblasts to periodontally involved root surface following scaling and/or etching procedures: A scanning electron microscopy study. Braz Dent J 2001; 12: 17-21.
5. Barkmeister WW, Jabro MH. Scanning electron microscopic analysis of the local effects of a periodontal scaling gel on selected surfaces. J Clin Dent 1992; 3:39-42.
6. Blomlöf J, Lindskog S. Periodontal tissue-vitality after different etching modalities. J Clin Periodontol 1995; 22: 464-468.
7. Al-Nazhan S. SEM observations on the attachment of Human periodontal ligament fibroblasts to non- mineralized dentine surface in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004; 97: 393-397.
8. Babay N. The effect of tetracycline hydrochloride on the attachment of gingival fibroblasts. A scanning electron microscopic study on the effect of ultrasonic scaling before root Conditioning. Odontostomatol Trop 2002; 25: 13-17.
9. Baker PJ, Rotch HA, Trombelli L, et al. An in vitro screening model to evaluate root-conditioning protocols for periodontal regenerative procedures. J Periodontol 2000; 71: 1139-1143.
10. Melcher AH. On the repair potential of periodontal tissues. J Periodontol 1976; 47: 256-260.
11. Aukhil I, Pettersson E. Effect of citric acid conditioning on fibroblast cell density in periodontal wounds. J Clin Periodontol 1987; 14: 80-84, 1987.
12. Cogen RB, Al-Joburi W, Gantt DG, et al. Effect of various root surface treatments on the attachment and growth of human gingival fibroblasts: histologic and scanning electron microscopic evaluation. J Clin Periodontol 1984; 11: 531-539.
13. Fardal O, Lowenberg BF. A quantitative analysis of the migration, attachment, and orientation of human gingival fibroblasts to human dental root surfaces in vitro. J Periodontol 1990; 61: 529-35.
14. Liskay RM. Genetic analysis of a Chinese hamster cell line lacking a G1 phase. Exp Cell Res 1978; 114: 69-77 .
15. World Workshop in Clinical Periodontics. Proceedings of the World Workshop in Clinical Periodontics. American Academy of Periodontology. 1989.
16. Jones WA, O'Leary TJ. The effectiveness of in vivo root planing in removing bacterial endotoxin from the root of periodontally involved teeth. J Periodontol 1978; 49: 337-342.
17. Polson AM, Frederick GT, Ladenheim S, et al. The production of root surface smear layer by instrumentation and its removal by citric acid. J Periodontol 1984; 55: 443-446, 1984.
18. Mariotti A. Efficacy of chemical root surface modifiers in the treatment of periodontal disease. A systematic review. Annals of Periodontology 2003; 8: 205-226.
19. Payne JM, Cobb CM, Rapley JW, et al. Migration of Human Gingival Fibroblasts over Guided Tissue Regeneration Barrier Materials. J Periodontol 1996; 67; 236-244.
20. Fernyhough W, Page RC. Attachment, growth and synthesis by human gingival fibroblasts on demineralized or fibronectin-treated normal and diseased tooth roots. J Periodontol 1983; 54: 133-140, 1983.
21. Wikesjo U, Crigger M, Nilveus R, et al. Early healing at the dentinal connective tissue interface-Light microscopy and Transmission electron microscopic study. J Periodontol 1991; 62: 5-14.
22. Lowenguth RA, Polson AM, Caton JG. Oriented cell and fiber attachment systems in vivo. J Periodontol 1993; 64: 330-42.
23. Vanheusden A, Nusgens B, Goffinet G, et al. In vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCL. J Periodontal Res 1998; 33: 377-85.
24. Gamal AY, Mailhot JM. The effects of EDTA gel conditioning exposure time on periodontitis-affected human root surfaces: surface topography and PDL cell adhesion. J Int Acad Periodontol 2003; 5: 11-22.
25. Pant V, Dixit J, Agrawal AK, et al. Behavior of human periodontal ligament cells on CO2 laser irradiated dentinal root surfaces: an in vitro study. J Periodontal Res 2004; 39: 373-379.
26. Zaman KU, Sugaya T, Hongo O, et al. A study of attached and oriented Human periodontal ligament cells to periodontally diseased cementum and dentine after demineralizing with neutral and low pH etching solutions. J Periodontol 2000; 71: 1094-9.
27. Polson AJ, Proye MP. Fibrin linkage: A precursor for new attachment. J Periodontol 1983; 54: 141-147.
28. Hanes PJ, O'Brien NJ, Garnick JJ. A morphological comparison of radicular dentine following treatment with tetracycline and citric acid. J Clin Periodontol 1991; 18: 660-668.
29. Rompen EH, Goffinet GH, Nusgens B. Human periodontal ligament fibroblast behavior on chemically conditioned dentine: an in vitro study. J Periodontol 1999; 70: 1144-1152.
30. Lafferty TA, Gher ME, Gray JL. Comparative SEM study on the effect of acid etching with tetracycline HCL and citric acid on instrumented periodontally involved root surfaces. J Periodontol 1993; 64: 689-693.
31. Rompen EH, Kohl J, Nusgens B, et al. Kinetic aspects of gingival and periodontal ligament fibroblast attachment to surface-conditioned dentin. J Dent Res 1993; 72: 607-12.
32. Van der Schueren B, Cassiman JJ, Van den Berghe H. Outgrowth of human fibroblast aggregates on a substratum triggers a wide variety of morphogenic properties in the cells. J Cell Sci 1977; 26: 101-107.
33. Lowenberg BF, Pilliar RM, Aubin JE, et al. Cell attachment of human gingival fibroblasts in vitro to porous-surfaced titanium alloy discs coated with collagen and platelet-derived growth factor. Biomaterials 1988; 9: 302-9.
34. V79 Cell Battery. GenPharmTox BioTech AG, 2000 (Electronic reference from http://www.genpharmtox.com/services/V79%20Cell%20Battery/cellbattery.html)