Oxygen supply for CHO cells immobilized on a packed-bed of Fibra-Cel® disks

Biotechnology and Bioengineering

Volumn 93, Issue 4, 2006, Pages 791-800

  Meuwly, F ^a   Loviat, F ^b   Ruffieux, P A ^c   Bernard, A R ^a   Kadouri, A ^a   Von Stockar, U ^d  

^a MERCK SERONO S A   (Switzerland)

^b PAUL SCHERRER INSTITUTE   (Switzerland)

^c NOVARTIS PHARMA AG   (Switzerland)

^d EPFL   (Switzerland)

Author keywords

CHO; Fibra Cel ; Oxygen; Packed bed; Perfusion

Indexed keywords

CELL IMMOBILIZATION; CONCENTRATION (PROCESS); MATHEMATICAL MODELS; OXYGEN;

CHO; FIBRA-CEL®; PACKED-BED; PERFUSION;

BIOREACTORS;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL CULTURE; CELL DENSITY; CHO CELL; CONTROLLED STUDY; GLUCOSE INTAKE; IMMOBILIZED CELL; NONHUMAN; OXYGEN CONSUMPTION; OXYGEN SUPPLY; OXYGENATION; PACKED BED REACTOR;

ANIMALS; BIOREACTORS; CELLS, IMMOBILIZED; CHO CELLS; CRICETINAE; CRICETULUS; MODELS, THEORETICAL; OXYGEN;

ANIMALIA; CRICETULUS GRISEUS; MAMMALIA;

EID: 33644913696     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20766     Document Type: Article

References (39)

1. Aris R. 1975. The mathematical theory of diffusion and reaction in permeable catalysts, Vol. 1. The theory of steady state. Oxford: Clarendon Press.
2. Bohak Z, Kadouri A. 1987. Novel anchorage matrices for suspension culture of mammalian cells. Biopolymers 26:S205-S213.
3. Caprez W, Javet P. 1980. Cours de phenomenes de transfert. 1:A.16.
4. Chiou T-W, Murakami S, Wang DIC. 1991. A fiber-bed bioreactor for anchorage-dependent animal cell cultures: Part I. Bioreactor design and operations. Biotechnol Bioeng 37:755-761.
5. Cochlo D, Thovert J-F, Adler PM. 1997. Geometrical and transport properties of random packings of spheres and aspherical particles. Phys Rev E 55:1959-1978.
6. Ducommun P, Bolzonella I, Rhiel M, Pugeaud P, von Stockar U, Marison IW. 2001. On-line determination of animal cell concentration. Biotechnol Bioeng 72:515-522.
7. Fassnacht D, Pörtner R. 1999. Experimental and theoretical considerations on oxygen supply for animal cell growth in fixed-bed reactors. J Biotechnol 72:169-184.
8. Flendrig LM, te Velde AA, Chamuleau RAFM. 1997. Semipermeable hollow fiber membranes in Hepatocyte bioreactors: A prerequisite for a successful bioartificial liver? Artif Organs 21:1177-1181.
9. Froment GF, Bischoff KB. 1962. Chemical reactor analysis and design. New York: John Wiley and Sons. 162p.
10. Jorjani P, Ozturk SS. 1999. Effects of cell density and temperature on oxygen consumption rate for different mammalian cell lines. Biotechnol Bioeng 64:349-356.
11. Kadouri A. 1994. Cultivation of anchorage-dependent mammalian cells and production of various metabolites. Colloids Surf B Biointerfaces 2:265-272.
12. Kawakubo Y, Matsushita T, Funatsu K. 1994. Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embyonic kidney cells using a polyurethane-foam packed-bed culture system. Appl Microbiol Biotechnol 41:413-418.
13. Kilburn DG, Webb FC. 1968. The cultivation of animal cells at controlled dissolved oxygen partial pressure. Biotechnol Bioeng 10:801-814.
14. Kilburn DG, Lilly MD, Self DA, Webb FC. 1969. The effect of dissolved oxygen partial pressure on the growth and carbohydrate metabolism of mousse LS cells. J Cell Sci 4:25-37.
15. Kurosawa H, Yasumoto K, Kimura T, Amano Y. 2000. Polyurethane membrane as an efficient immobilization carrier for high-density culture of rat hepatocytes in the fixed-bed reactor. Biotechnol Bioeng 70:160-166.
16. Levenspiel O. 1999. Chemical Reaction Engineering. New York: John Wiley & Sons.
17. Meuwly F, Ruffieux P-A, Kadouri A, von Stockar U. 2005. Packed-bed bioreactors for mammalian cell culture: Bioprocess and biomedical applications. Biotech Progress (submitted).
18. Miller WM, Wilke CR, Blanch HW. 1987. Effects of dissolved oxygen concentration on hybridoma growth and metabolism in continuous culture. J Cell Physiol 132:524-530.
19. Mitsuda S, Matsuda Y, Kobayashi N, Suzuki A, Itagaki Y, Kumazawa E, Higashio K, Kawanishi G. 1991. Continuous production of tissue plasminogen activator (t-PA) by human embryonic lung diploid fibroblast, IMR-90 cells, using ceramic bed reactor. Cytotechnology 6:23-31.
20. Murakami S, Chiou T-W, Wang DIC. 1991. A fiber-bed bioreactor for anchorage-dependent animal cell cultures: Part II. Scaleup potential. Biotechnol Bioeng 37:762-769.
21. Nielsen J, Villadsen J. 1994. Bioreaction engineering principles. New York: Plenum Press.
22. Nienow AW, Langheinrich C, Eddleston T, Stevenson NC, Emery AN, Clayton TM, Slater NKH. 1996. Homogenisation and oxygen transfer rates in large agitated and sparged animal cell bioreactors: Some applications for growth and production. Cytotechnology 22:87-94.
23. Ohashi H, Sugawara J, Kikuchi K, Konno H. 1981. Correlation of liquid-side mass transfer coefficient for single particle and fixed beds. J Chem Eng Jpn 14:433-438.
24. Ong CP, Pörtner R, Märkl H, Yamazaki Y, Yasuda K, Matsumura M. 1994. High density cultivation of hybridoma in charged porous carriers. J Biotechnol 34:259-268.
25. Ozturk SS, Palsson BO. 1990. Effects of dissolved oxygen on hybridoma cell growth, metabolism, and antibody production kinetics in continuous culture. Biotechnol Prog 6:437-446.
26. Park S, Stephanopoulos G. 1993. Packed bed bioreactor with porous ceramic beads for animal cell culture. Biotechnol Bioeng 41:25-34.
27. Perry RH, Green DW. 1997. Perry's chemical engineers' handbook (Chapter 5). New York: McGraw-Hill.
28. Perry ST, Wang DIC. 1989. Fiber bed reactor design for animal cell culture. Biotechnol Bioeng 34:1-9.
29. Pörtner R, Koop M. 1997. A model for oxygen supply in fixed bed reactors with immobilized hybridoma cells. Bioprocess Eng 17:269-275.
30. Pörtner R, Rössing S, Koop M, Lüdemann I. 1997. Kinetic studies on hybridoma cells immobilized in fixed bed reactors. Biotechnol Bioeng 55:535-541.
31. Racher AJ, Griffiths JB. 1993. Investigation of parameters affecting a fixed bed bioreactor process for recombinant cell lines. Cytotechnology 13:125-131.
32. Racher AJ, Looby D, Griffiths JB. 1990. Studies on monoclonal antibody production by a hybridoma cell line (C1E3) immobilised in a fixed-bed, porosphere culture system. J Biotechnol 15:129-146.
33. Serrato JA, Palomares LA, eneses-Acosta A, amírez OT. 2004. Heterogeneous conditions in dissolved oxygen affect N-glycosylation but not productivity of a monoclonal antibody in hybridoma cultures. Biotechnol Bioeng 88:176-188.
34. Thiele EW. 1939. Relation between catalytic activity and size of particle. Ind Eng Chem 31:916-920.
35. Wang G, Zhang W, Jacklin C, Freedman D, Eppstein L, Kadouri A. 1992. Modified CelliGen-packed bed bioreactors for hybridoma cell cultures. Cytotechnology 9:41-49.
36. Weiss RF. 1970. The solubility of nitrogen, oxygen, and argon in water and seawater. Deep Sea Res 17:721-735.
37. Wilke CR, Miller WM. 1987. Effects of oxygen dissolved concentration on hybridoma growth and metabolism in continous culture. J Cell Physiol 132:524-530.
38. Yanagi K, Miyoshi H, Fukuda H, Ohshima N. 1992. A packed-bed reactor utilizing porous resin enables high-density culture of hepatocytes. Appl Microbiol Biotechnol 37:316-320.
39. Yoon S, Konstantinov KB. 1994. Continuous, real-time monitoring of the oxygen-uptake rate (OUR) in animal-cell bioreactors. Biotechnol Bioeng 44:983-990.