Determination of diffusion coefficients of proteins in stationary phases by frontal chromatography

Biotechnology and Bioengineering

Volumn 93, Issue 4, 2006, Pages 656-664

  Kempe, Henrik ^a,b   Persson, Patrik ^a   Axelsson, Anders ^a   Nilsson, Bernt ^a   Zacchi, Guido ^a  

^a Department of Chemical Engineering ^*  (Sweden)

^b Experimental Medical Science   (Sweden)

Author keywords

Chromatography; Diffusion; Downstream processing; Effective diffusion coefficient; Frontal chromatography; Protein separation

Indexed keywords

LIQUID CHROMATOGRAPHY; MASS TRANSFER; MATHEMATICAL MODELS; PHASE SEPARATION; PROTEINS;

DOWNSTREAM PROCESSING; EFFECTIVE DIFFUSION COEFFICIENT; FRONTAL CHROMATOGRAPHY; PROTEIN SEPARATION;

DIFFUSION;

BOVINE SERUM ALBUMIN; IMMUNOGLOBULIN; LYSOZYME;

ARTICLE; CHROMATOGRAPHY; DIFFUSION COEFFICIENT; DISPERSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MATHEMATICAL MODEL; METHODOLOGY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DETERMINATION;

ANIMALS; CATTLE; CHICKENS; CHROMATOGRAPHY; DIFFUSION; IMMUNOGLOBULIN G; MICE; MODELS, CHEMICAL; MURAMIDASE; SERUM ALBUMIN, BOVINE;

BOVINAE;

EID: 33644913140     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20738     Document Type: Article

References (23)

1. Bosma JC, Wesselingh JA. 2000. Partitioning and diffusion of large molecules in fibrous structures. J Chromatogr B 743:169-180.
2. Boyer PM, Hsu JT. 1992. Experimental studies of restricted protein diffusion in an agarose matrix. AIChE J 38(2):259-272.
3. Cadman AD, Fleming R, Guy RH. 1982. Diffusion of lysozyme chloride in water and aqueous potassium chloride solutions. Biophys J 37:569-574.
4. Coffman JL, Lightfoot EN, Root TW, Placidi M, Cannistraro S. 1997. Protein diffusion in porous chromatographic media studied by proton and PFG-NMR. J Phys Chem 101:2218-2223.
5. Fernandez-Sousa JM, Rodriguez R. 1977. Lysozyme-agarose interaction. Biochem Biophys Res Commun 74(4):1426-1431.
6. Foo SC, Rice RG. 1975. Prediction of ultimate separation in parametric pumps. AIChE J 21:1149.
7. Fuh CB, Levin S, Giddings JC. 1993. Rapid diffusion coefficient measurements using analytical SPLITT fractionation: Application to proteins. Anal Biochem 208:80-87.
8. 2 solutions from dynamic light-scattering data: Effect of protein and salt concentrations. J Phys Chem B 104:3645-3650.
9. Kempe H, Axelsson A, Nilsson B, Zacchi G. 1999. Simulation of chromatographic processes applied to separation of proteins. J Chromatogr A 846:1-12.
10. Khramtsov VV, Marsh D. 1991. Measurement of the local translational diffusion rates of proteins by saturation transfer EPR spectroscopy. Biochim Biophys Acta 1068(2):257-260.
11. Kim Y-C, Myerson AS. 1996. Diffusivity of protein in aqueous solutions. Korean J Chem Eng 13(3):288-293.
12. Larew LA, Walters RR. 1987. A kinetic, chromatographic method for studying protein hydrodynamic behavior. Anal Biochem 164:537-546.
13. Levenspiel O. 1972. Chemical reaction engineering. New York: Wiley.
14. Liu M-K, Li P, Giddings JC. 1993. Rapid protein separation and diffusion coefficient measurement by frit inlet flow field-flow fractionation. Protein Sci 2:1520-1531.
15. Mattisson C. 1999. Diffusion studies in gels using holographic laser interferometry, Ph.D. thesis, LUTHDKH/TKKA-1003, Chem. Eng. 1. Lund University, Lund, Sweden.
16. Mattisson C, Roger P, Jönsson B, Axelsson A, Zacchi G. 2000. Diffusion of lysozyme in gels and liquids: A general approach for the determination of diffusion coefficients using holographic laser interferometry. J Chromatogr B: Biomed Sci Appl 743:151-167.
17. Merril EW, Dennison KA, Sung C. 1993. Partitioning and diffusion of solutes in hydrogels of poly(ethylene oxide). Biomaterials 14(15):1117-1126.
18. Moussaoui M, Benlyas M, Wahl P. 1992. Diffusion of proteins in sepharose C1-B gels. J Chromatogr 591:115-120.
19. Placidi M, Cannistraro S. 1998. A dynamic light scattering study on mutual diffusion coefficient of BSA in concentrated aqueous solutions. Europhys Lett 43(4):476-481.
20. Roger P, Mattisson C, Axelsson A, Zacchi G. 2000. Use of holographic laser interferometry to study the diffusion of polymers in gels. Biotechnol Bioeng 69(6):654-663.
21. Sherwood TK, Pigford RL, Wilke CR. 1975. Mass transfer. New York: McGrawHill.
22. Westrin B. 1991. Diffusion measurements in gels: A methodological study, Ph. D. thesis, LUTKDH/TKKA-1003, Chem. Eng. 1. Lund University, Lund, Sweden.
23. Zhang X, Hirota N, Narita T, Gong JP, Osada Y, Chen K. 1999. Investigation of molecular diffusion in hydrogel by electronic speckle pattern interferometry. J Phys Chem B 103:6069-6074.