Enhanced stability of recombinant keratinocyte growth factor by mutagenesis

Protein Engineering, Design and Selection

Volumn 19, Issue 4, 2006, Pages 147-153

  Hsu, Eric ^a   Osslund, Timothy ^a   Nybo, Rebecca ^a   Chen, Bao Lu ^c   Kenney, William C ^a   Morris, C Fred ^a   Arakawa, Tsutomu ^b   Narhi, Linda O ^a  

^a AMGEN INC   (United States)

^b ALLIANCE PROTEIN LABORATORIES   (United States)

^c NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Cysteine replacement; Heparin binding; KGF; Protein stability

Indexed keywords

AMINO ACIDS; CULTIVATION; NITROGEN; PROTEINS;

CYSTEINE REPLACEMENT; HEPARIN BINDING; KGF; PROTEIN STABILITY;

MUTAGENESIS;

AMINO ACID DERIVATIVE; ARGININE; CYSTEINE; DISULFIDE; GLUCOSE; GLUTAMIC ACID; RECOMBINANT KERATINOCYTE GROWTH FACTOR;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOACCUMULATION; CHEMICAL BOND; CONTROLLED STUDY; GENE DELETION; GENE MUTATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN STABILITY; TEMPERATURE DEPENDENCE;

AMINO ACID SEQUENCE; ANIMALS; CALORIMETRY, DIFFERENTIAL SCANNING; CELL LINE; CIRCULAR DICHROISM; DRUG STABILITY; FIBROBLAST GROWTH FACTOR 7; HEAT; HEPARIN; MICE; MITOGENS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DENATURATION; RECOMBINANT PROTEINS;

AMINO ACIDS; CULTIVATION; DISULFIDES; KERATIN; MUTAGENS; MUTATION; NITROGEN; PROTEINS;

EID: 33644893106     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzj013     Document Type: Article

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