Three-dimensional characterization of cell clusters using synchrotron-radiation-based micro-computed tomography

Microscopy and Microanalysis

Volumn 12, Issue 2, 2006, Pages 97-105

  Müller, Bert ^a   Riedel, Marco ^b   Thurner, Philipp J ^c  

^a ETH ZURICH   (Switzerland)

^b PROBIOGEN AG   (Germany)

^c SWISS FEDERAL LABORATORIES FOR MATERIALS SCIENCE AND TECHNOLOGY   (Switzerland)

Author keywords

3D characterization; DNA RNA staining; HEK 293 cell cluster; Micro computed tomography; Segmentation; Synchrotron radiation

Indexed keywords

DNA; GLUTARALDEHYDE; RNA;

ARTICLE; CELL LINE; CHEMISTRY; COMPUTER ASSISTED TOMOGRAPHY; CONFORMATION; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; HUMAN; KIDNEY; METHODOLOGY; PHOTON; SYNCHROTRON; ULTRASTRUCTURE;

CELL CULTURE TECHNIQUES; CELL LINE; CULTURE MEDIA, SERUM-FREE; DNA; GLUTARAL; HUMANS; KIDNEY; MOLECULAR CONFORMATION; PHOTONS; RNA; SYNCHROTRONS; TOMOGRAPHY, X-RAY COMPUTED;

EID: 33644849989     PISSN: 14319276     EISSN: 14358115     Source Type: Journal    
DOI: 10.1017/S1431927606060168     Document Type: Article

References (24)

1. ABBOTT, A. & CYRANOSKI, D. (2003). Biology's new dimension. Nature 424, 870-872.
2. BECKMANN, F. (2001). Microtomography using synchrotron radiation as a user experiment at beamlines BW2 and BW5 of HASYLAB at DESY. In Developments in X-ray Tomography III, Bonse, U. (Ed.), pp. 34-41. San Diego: SPIE - The International Society for Optical Engineering.
3. BERNHARDT, R., SCHARNWEBER, D., MÜLLER, B., THURNER, P., SCHLIEPHAKE, H., WYSS, P., BECKMANN, F., GOEBBELS, J. & WORCH, H. (2004). Comparison of microfocus- and synchrotron X-ray tomography for the analysis of osteointegration around Ti6Al4V-implants. Eur Cell Mater 7, 42-51.
4. BONSE, U. & BUSCH, F. (1996). X-ray computed microtomography (μCT) using synchrotron radiation. Prog Biophys Mol Biol 65, 133-169.
5. BONSE, U. & HART, M. (1965a). An X-ray interferometer. Appl Phys Lett 6, 155-156.
6. BONSE, U. & HART, M. (1965b). An X-ray interferometer with long separated interfering beam paths. Appl Phys Lett 7, 99-100.
7. CASCIARI, J.J., SOTIRCHOS, S.V. & SUTHERLAND, R.M. (1992). Mathematical modelling of microenvironment and growth in EMT6/Ro multicellular tumour spheroids. Cell Prolif 25, 1-22.
8. HILDEBRAND, T. & RÜEGSEGGER, P. (1997). A new method for the model-independent assessment of thickness in three-dimensional images. J Microsc 185, 67-75.
9. HOFFMAN, R.M. (1993). To do tissue culture in two or three dimensions? That is the question. Stem Cells 11, 105-111.
10. KAK, A.C. & SLANEY, M. (2001). Principles of Computerized Tomographic Imaging. Philadelphia, PA: The Society of Industrial and Applied Mathematics.
11. KÖNIG, D., CARVAJAL-GONZALEZ, S., DOWNS, A.M., VASSY, J. & RIGAUT, J.P. (1991). Modelling and analysis of 3-D arrangements of particles by point processes with examples of application to biological data obtained by confocal scanning light microscopy. J Microsc 161, 405-433.
12. LAURENT, M., JOHANNIN, G., GILBERT, N., LUCAS, L., CASSIO, D., PETIT, P.X. & FLEURY, A. (1994). Power and limits of laser scanning confocal microscopy. Biol Cell 80, 229-240.
13. LEVIN-ZAIDMAN, S., FRENKEL-KRISPIN, D., SHIMONI, E., SABANAY, I., WOLF, S.G. & MINSKY, A. (2000). Ordered intracellular reca-DNA assemblies: A potential site of in vivo reca-mediated activities. Proc Natl Acad Sci USA 97, 6791-6796.
14. LIU, M., XU, J., SOUZA, P., TANSWELL, B., TANSWELL, A.K. & POST, M. (1995). The effect of mechanical strain on fetal rat lung cell proliferation: Comparison of two- and three-dimensional culture systems. In Vitro Cell Dev Biol Anim 31, 858-866.
15. MARQUARDT, D.W. (1969). An algorithm for least-squares estimation of nonlinear parameters. J Soc Ind Appl Math 11, 431-441.
16. MÜLLER, B., BECKMANN, F., HUSER, M., MASPERO, F., SZEKELY, G., RUFFIEUX, K., THURNER, P. & WINTERMANTEL, E. (2002). Non-destructive three-dimensional evaluation of a polymer sponge by micro-tomography using synchrotron radiation. Biomol Eng 19, 73-78.
17. MÜLLER, B. & WINTERMANTEL, E. (1999). Ein strukturkompatibler Werkstoff für die Leberzelltransplantation. Bulletin 274, 42-45.
18. MÜLLER-KLIESER, W. (1987). Multicellular spheroids. J Cancer Res Clin Oncol 113, 101-122.
19. STAMPANONI, M., WYSS, P., ABELA, R., BORCHERT, G., VERMEULEN, D. & RÜEGSEGGER, P. (2001). X-ray tomographic microscopy at the Swiss light source. In Developments in X-ray Tomography III, Bonse, U. (Ed.), pp. 42-53. San Diego: SPIE - The International Society for Optical Engineering.
20. SUZUKI, K., HORIBA, I. & SUGIE, N. (2003). Linear-time connected-component labeling based on sequential local operations. Comput Vision Image Understand 89, 1-23.
21. TAKEZAWA, T. (2003). A strategy for the development of tissue engineering scaffolds that regulate cell behavior. Biomaterials 24, 2267-2275.
22. THURNER, P., BECKMANN, F. & MÜLLER, B. (2004). An optimization procedure for spatial and density resolution in hard X-ray micro-computed tomography. Nucl Instrum Meth Phys Res B 225, 599-603.
23. THURNER, P., MÜLLER, B., BECKMANN, F., WEITKAMP, T., RAU, C., MÜLLER, R., HUBBELL, J.A. & SENNHAUSER, U. (2003). Tomography study of human foreskin fibroblasts on polymer yarns. Nucl Instrum Meth Phys Res B 200, 397-405.
24. WEAVER, V.M., PETERSEN, O.W., WANG, F., LARABELL, C.A., BRIAND, P., DAMSKY, C. & BISSELL, M.J. (1997). Reversion of the malignant phenotype of human breast cells in three-dimensional culture and in vivo by integrin blocking antibodies. J Cell Biol 137, 231-245.