Detection of intestinal microsporidiosis in diarrhoeal patients infected with the human immunideficiency virus (HIV-1) using PCR and Uvitex-2B stain

Ethiopian Medical Journal

Volumn 43, Issue 2, 2005, Pages 97-101

  Endeshaw, Tekola ^a   Kebede, Amha ^a   Verweij, Jaco J ^b   Wolday, Dawit ^a   Zewide, Ayele ^c   Tsige, Kebede ^d   Abraham, Yodit ^d   Messele, Tsehaynesh ^a   Polderman, Anton M ^b   Petros, Beyene ^e  

^a ETHIOPIAN PUBLIC HEALTH INSTITUTE   (Ethiopia)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^c Police Force General Hospital   (Ethiopia)

^d Armed Force General Hospital

^e ADDIS ABABA UNIVERSITY   (Ethiopia)

Author keywords

[No Author keywords available]

Indexed keywords

ADOLESCENT; ADULT; AIDS RELATED COMPLEX; ANIMAL; ARTICLE; DIARRHEA; ENCEPHALITOZOON; ENTEROCYTOZOON; ENTEROPATHY; ETHIOPIA; FEMALE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; ISOLATION AND PURIFICATION; MALE; MICROBIOLOGY; MICROSPORIDIOSIS; POLYMERASE CHAIN REACTION; STAINING; WASTING SYNDROME;

ADOLESCENT; ADULT; AIDS-RELATED OPPORTUNISTIC INFECTIONS; ANIMALS; DIARRHEA; ENCEPHALITOZOON; ENTEROCYTOZOON; ETHIOPIA; FEMALE; HIV WASTING SYNDROME; HIV-1; HUMANS; INTESTINAL DISEASES; MALE; MICROSPORIDIOSIS; POLYMERASE CHAIN REACTION; STAINING AND LABELING;

EID: 33644841249     PISSN: 00141755     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Weber R, Bryan RT, Shruratz AD, Owen RL. Human microsporidal infection. Clin. Microbiol. Rev. 1994;7(4): 426-61.
2. Bryan TB. Microsporidosis as an AIDS related oppurtunistic infections. Clin. Infect. Dis. 1995; 21(suppl. 1): 562-5.
3. LookWood JND, Weber JN. Parasite infection in AIDS. Parasitol. Today. 1989; 5(10): 310-15.
4. Chukwuma C. Microsporidia in AIDS patients: A Prospective. E. Af. Med. J. 1996; 73(1): 72-5.
5. Goodgame WR, Stager C, Marcantel B, Alcoler E, Segura AM. Intensity of infection in AIDS related intestinal microsporidiosis. J. Infect. Dis. 1999; 180: 929-32.
6. Sianongo S, MacDonald V, Kelly P. A method for diagnosis of microsporidiosis adapted for use in developing countries. Trans. Roy. Soc. Trop. Med. Hyg. 2001; 95: 605-7.
7. Drobnieruski F, Kelly P, Carew A, Ngwenya B, Luo N, Pankhurst C, Farthing M. Human microsporidiosis in African AIDS patients with chronic diarrhoea. J. Infect. Dis. 1995;171:515-6.
8. Conteas NC, Berlin WGO, Speck EE, Pundhumas SS, Lariviere MJ, Fu C. Modification of the clinical course of intestinal microsporidiosis in AIDS patients by immune status and anti-HVI Therapy. Am. J. Trop. Med. Hyg. 1998;58(5): 555-8.
9. DeGirolami GP, Ezratty RC, Desal G. Diagnosis of intestinal microsporidiosis by the examination of stool and duodenal aspirates with Weber's modified trichome and Uvitex-2B stains. J. Clin. Microbiol. 1995; 33(4): 800-10.
10. Kotler DP, Orenstein JM. Clinical syndromes associated with microsporidiosis. Adv. Parasitol. 1998; 40: 322-43.
11. Garcia LS. Laboratory Identification of the microsporidia. J. Clin. Microbiol. 2002; 40(6): 1892-901.
12. Desportes I, Charpentier Y, Galian AOccurrence of a new microsporidia: Enterocylozoon bieneusi n.g., n. sp. In the entrocytes of a human patients with AIDS. J. Protozoal. 1985;32: 250-4.
13. Lucas BS, Papadaki L, Conlon C. Diagnosis of intestinal microsporidiosis with AIDS. J. Clin Pathol. 1989; 42: 885-90.
14. Dallabetta AG, Miotti GP. Chronic diarrhoea in AIDS patients in the tropics: A Review. Trop. Doc. 1992; 22. 3-9.
15. Dascomb K, Clark R, Aberg J, Pulvienti J, Hewitt RG, Kissinger P, Dider ES. Natural history of intestinal microsporidiosis among patients infected with HIV. J. Clin. Microbiol. 1999; 37: 3421-2.
16. Asmuth MD, DeGirolami CP, Federman M, Ezratty CR, Pleskow DK, Desai G, Wanke CA. Clinical features of microsporidiosis in patients with AIDS. Clin. Infect. Dis. 1994; 18: 819-25.
17. Navin RT, Weber R, Vugia JD, Rimland D, Roberts JM, Addis GD, et al. Declining CD4+ T-lymphocyte counts are associated with increased risk of enteric parasitosis and chronic diarrhoea: Results of a 3-year longitudinal study. J. Acqu. Immuno Def. Syndrome & Human Retrovirol. 1999; 20(2): 154-9.
18. Van Gool T, Lundehoff E, Nathuoo KJ, Kiire CF, Dankert J, Manson PR. High prevalence of Enterocylozoon bieneusi infections among HIV-positive individuals with persistent diarrhoea in Harare, Zimbabwe. Trans. Roy. Soc. Trop. Med. Hyg. 1995; 89: 478-80.
19. Gumbo T, Sarbah S, Gangaidoz TT. Intestinal parasites in patients with diarrhoea and HIV infected in Zimbabwe. AIDS. 1999; 13: 819-822.
20. Van Gool T., Cunning E., Dankert J. An improved practical and sensitive technique for the detection of microsporidia spores in stool samples. Trans. Roy. Soc. Trop. Med. Hyg. 1994; 88:189-190.
21. Verweij JJ, Pits DS., Van Lieshout L., Baeta SM, Dery GD, Casser RB, Polderman AM. Determining the prevalence of Oesophagastum bifurcum and Necator americanus infections using specific PCR amplification of DNA from faecal samples. Trop. Med Intern. Health. 2000; 6: 726-31.
22. Katzwnikel-Wladarsch S, Lieb M, Heise W, Loscher T, Rinder H. Direct amplification and species determination of microsporidia DNA from stool specimens. Trop. Med. Inten. Helth. 1996;1(3): 373-8.
23. Rebeneck L, Gyorkey F, Genta RM, Gyorkes P, Foote LW, Risser MH. The role of microsporidia in the pathogenesis of HIV related chronic diarrhoea. Ann. Intern. Med. 1993; 119: 895-9.
24. Germani Y, Minssart P, Vohito M, Yassibanda S, Glazion P, Hocqvet D, et al. Aetiologies of acute, persistent and dysenteric diarrhoea in adults in Bangui, Central Africa Republic, in relation to HIV-serostatus. Am. J. Trop. Med. Hyg. 1998; 59(6): 1008-14.
25. Tarimo SD, Killeno JZJ, Mirij AS, Masmanga IG. Prevalence of intestinal parasites in adults patients with entropathic AIDS in North-Eastern Tanzania. E. Af. Med J. 1996; 7(6): 397-9.
26. Fedorko DP., Hiyazi YM. Application of molecular techniques to the diagnosis of microsporidial infection. Emerg. Infect. Dis. 1996; 2(3): 183-91.
27. Molina JM, Oksenhender E, Beavuais B, Sarfati C, Jaccord A, Derouin N, Moday J. Disseminated microsporidiosis due to Septata intestinalis in patients with AIDS: Clinical features and response to Albendazole therapy. J. Infect. Dis. 1995;171: 245-9.
28. Conteas NC, Berlin WGO, Ash LR, Peruth JS. Therapy for human gastrointestinal microsporidiosis. Am. J. Trop. Med. Hyg. 2000; 63(3,4): 121-7.