Reactive oxygen species generated by mitochondrial injury in human brain microvessel endothelial cells

Clinical Hemorheology and Microcirculation

Volumn 34, Issue 1-2, 2006, Pages 163-168

  Itoh, Yoshiaki ^a   Takaoka, Rie ^a   Ohira, Masayuki ^a   Abe, Takato ^a   Tanahashi, Norio ^b   Suzuki, Norihiro ^a  

^a KEIO UNIVERSITY   (Japan)

^b SAITAMA MEDICAL UNIVERSITY   (Japan)

Author keywords

Dichlorodihydrofluorescein; Endothelial cell; Human brain; Microvessel; Reactive oxygen species (ROS)

Indexed keywords

CARBONYL CYANIDE CHLOROPHENYLHYDRAZONE; DICHLORODIHYDROFLUORESCEIN DIACETATE; NORPHENAZONE; REACTIVE OXYGEN METABOLITE; ROTENONE;

ANALYTIC METHOD; BRAIN MICROCIRCULATION; BRAIN MITOCHONDRION; CELL HYPOXIA; CONCENTRATION (PARAMETERS); CONFERENCE PAPER; CONTROLLED STUDY; DISORDERS OF MITOCHONDRIAL FUNCTIONS; ENDOTHELIUM CELL; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; HYDROLYSIS; IMAGE ANALYSIS; IMAGING SYSTEM; INCUBATION TIME; MICROVASCULATURE; MITOCHONDRIAL RESPIRATION; RESPIRATORY CHAIN;

ANOXIA; ANTIPYRINE; BRAIN; BRAIN ISCHEMIA; CEREBROVASCULAR CIRCULATION; ENDOTHELIUM, VASCULAR; FREE RADICAL SCAVENGERS; HUMANS; MICROCIRCULATION; MITOCHONDRIA; REACTIVE OXYGEN SPECIES; UNCOUPLING AGENTS;

EID: 33644797876     PISSN: 13860291     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (11)

1. S. Watanabe, In vivo fluorometric measurement of cerebral oxidative stress using 2′-7′-dichlorofluorescein (DCF), Keio J. Med. 47 (1998), 92-98.
2. K. Yamaguchi, D. Uematsu, Y. Itoh, S. Watanabe and Y. Fukuuchi, In vivo measurement of superoxide in the cerebral cortex during anoxia-reoxygenation and ischemia-reperfusion, Keio J. Med. 51 (2002), 201-207.
3. K.K. Griendling, D. Sorescu and M. Ushio-Fukai, NAD(P)H oxidase: role in cardiovascular biology and disease, Circ. Res. 86 (2000), 494-501.
4. S. Kimura, G.X. Zhang, A. Nishiyama, T. Shokoji, L. Yao, Y.Y. Fan, M. Rahman, T. Suzuki, H. Maeta and Y. Abe, Role of NAD(P)H oxidase- and mitochondria-derived reactive oxygen species in cardioprotection of ischemic reperfusion injury by angiotensin II, Hypertension 45 (2005), 860-866.
5. O. Myhre, J.M. Andersen, H. Aarnes and F. Fonnum, Evaluation of the probes 2′,7′-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation, Biochem. Pharmacol. 65 (2003), 1575-1582.
6. K. Setsukinai, Y. Urano, K. Kakinuma, H.J. Majima and T. Nagano, Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species, J. Biol. Chem. 278 (2003), 3170-3175.
7. K. Takeshige and S. Minakami, NADH- and NADPH-dependent formation of superoxide anions by bovine heart submitochondrial particles and NADH-ubiquinone reductase preparation, Biochem. J. 180 (1979), 129-135.
8. G. Lenaz, C. Bovina, M. D'Aurelio, R. Fato, G. Formiggini, M.L. Genova, G. Giuliano, M. Merlo Pich, U. Paolucci, G. Parenti Castelli and B. Ventura, Role of mitochondria in oxidative stress and aging, Ann. N.Y. Acad. Sci. 959 (2002). 199-213.
9. K. Abe, S. Yuki and K. Kogure, Strong attenuation of ischemic and postischemic brain edema in rats by a novel free radical scavenger, Stroke 19 (1988), 480-485.
10. C. Richter, Do mitochondrial DNA fragments promote cancer and aging?, FEBS Lett. 241 (1988), 1-5.
11. P. Mayes and K. Botham, The respiratory chain and oxidative phosphorylation, in: Harper's Illustrated Biochemistry, 26th edn, R. Murray, D. Granner, P. Mayes and V. Rodwell, eds, Lange Medical Books/McGraw-Hill, New York, 2003, pp. 92-101.