Constitutive acetonitrile hydrolysing activity of Nocardia globerula NHB-2: Optimization of production and reaction conditions

Indian Journal of Experimental Biology

Volumn 44, Issue 3, 2006, Pages 240-245

  Kumar, H ^a   Prasad, S ^a   Raj, J ^a   Bhalla, T C ^a  

^a HIMACHAL PRADESH UNIVERSITY   (India)

Author keywords

Acetonitrilase; Amidase; Nitrile hydratase; Nocardia globerula NHB 2

Indexed keywords

ACETONITRILE; ACETONITRILE DERIVATIVE; ION; METAL;

ARTICLE; CATALYSIS; CHEMISTRY; CULTURE MEDIUM; DRUG EFFECT; ENZYME SPECIFICITY; HYDROLYSIS; METABOLISM; NOCARDIA; PH; TEMPERATURE; TIME;

ACETONITRILES; CATALYSIS; CULTURE MEDIA; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; IONS; METALS; NOCARDIA; SUBSTRATE SPECIFICITY; TEMPERATURE; TIME FACTORS;

NOCARDIA; NOCARDIA GLOBERULA;

EID: 33644788155     PISSN: 00195189     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Nagasawa T, Methew C D, Mauger J & Yamada H, Nitrile hydratase-catalysed production of nicotinamide from 3-cyanopyridine in Rhodococcus rhodochrous J1. Appl Environ Microbiol, 54 (1988) 1766.
2. Nagasawa T, Nanba H, Ryuno K, Takeuchi K & Yamada H, Nitrile hydratase of Pseudomonas chlororaphis B23-purification and characterization. Eur J Biochem, 162 (1987) 691.
3. Kato Y, Ooi R & Asano Y, Isolation and characterization of a bacterium possessing a novel aldoxime-dehydration activity and nitrile-degrading enzymes. Arch Microbiol, 170 (1998) 85.
4. Bhalla T C, Miura A, Wakamoto A, Ohba Y & Furuhashi K, Asymmetric hydrolysis of α-aminonitriles to optically active amino acids by a nitrilase of Rhodococcus rhodochrous PA-34. Appl Microbiol Biotechnol, 37 (1992) 184.
5. Prasad S, Raj J & Bhalla T C, Optimisation of culture conditions for hyper production of nitrile hydratase by Rhodococcus rhodochrous PA-34. Indian J Microbiol, 44 (2004) 251.
6. Chand D, Kumar H, Sankhian U D, Kumar D, Vitzthum F & Bhalla T C, Treatment of simulated wastewater containing toxic amides by immobilized Rhodococcus rhodochrous NHB-2 using a highly compact 5-stage plug flow reactor. World J Microbiol Biotechnol, 20 (2004) 679.
7. Prepechalova I, Martinkova L, Stolz A, Ovesna M & Bezouska K, Purification and characterization of the enantioselective nitrile hydratase from Rhodococcus equi A4. Appl Microbiol Biotechnol, 55 (2001) 150.
8. Nagasawa T, Ryuno K & Yamada H, Nitrile hydratase of Brevibacterium R312-purification and characterization. Biochem Biophys Res Commun, 139 (1986) 1305.
9. O'grady D & Pembroke J T, Isolation of a novel Agrobacterium sp. capable of degrading a range of nitrile compounds. Biotechnol Lett, 16 (1994) 47.
10. Asano Y, Fujishiro K, Tani Y & Yamada H, Aliphatic nitrile hydratase from Arthrobacter sp. J-1: Purification and characterization. Agric Biol Chem, 46 (1982) 1165.
11. Linardi V R, Dias J C T & Rosa C A, Utilization of acetonitrile and other aliphatic nitriles by a Candida famata strain. FEMS Microbiol Lett, 144 (1996) 67.
12. Digeronimo M J & Antoine A D, Metabolism of acetonitrile and propionitrile by Nocardia rhodochrous LL100-21. Appl Environ Microbiol, 31 (1976) 900.
13. Acharya A & Desai A J, Studies on utilization of acetonitrile by Rhodococcus erythropolis A 10. World J Microbiol Biotechnol, 13 (1997) 175.
14. Langdahl B R, Bisp P & Ingvorsen K, Nitrile hydrolysis by Rhodococcus erythropolis BLI, an acetonitrile-tolerant strain isolated from a marine sediment. Microbiology, 142 (1996) 145.
15. Miller J M & Gray D O, The utilization of nitriles and amides by a Rhodococcus sp., J Gen Microbiol, 128 (1982) 1803.
16. Bhalla T C & Kumar H, Nocardia globerula NHB-2: A versatile nitrile-degrading organism. Can J Microbiol, 51 (2005) 705.
17. Watanabe I, Satoh Y, Enomoto K, Seki S & Sakashita K, Optimal conditions for cultivation of Rhodococcus sp. N-774 and for conversion of acrylonitrile to acrylamide by resting cells. Agric Biol Chem, 51 (1987) 3201.
18. Fawcett J K & Scott J E, A rapid and precise method for the determination of urea. J Clin Pathol, 13 (1960) 156.
19. Kopf M A, Bonnet D, Artaud I, Petre D & Mansuy D, Key role of alkanoic acids on the spectral properties, activity and active site stability of iron containing nitrile hydratase from Brevibacterium sp. R 312. Eur J Biochem, 240 (1996) 239.
20. Cramp R, Gilmour M & Cowan D A, Novel thermophilic bacteria producing nitrile-degrading enzymes. Microbiology, 143 (1997) 2313.
21. Andhale M S & Hamde V S, Isolation and screening of acrylamide producing microorganisms. Indian J Exp Biol, 34 (1996) 1005.
22. Prasad S, Sharma D R & Bhalla T C, Nitrile- and amide-hydrolysing activity in Kluyveromyces thermotolerans MGBY 37. World J Microbiol Biotechnol, (2005) (in press)
23. Jallageas J C, Arnaud A & Galzy P, Bioconversion of nitriles and their applications. Adv Biochem Engg, 14 (1980) 1.
24. Bollag D M & Edelstein S J, Preparation for protein isolation, in Protein methods, (John Wiley & Sons, New York) 1991, 1.