Optimisation of the amino-carboxy coupling of oligonucleotides to beads used in liquid arrays

Journal of Chemical Technology and Biotechnology

Volumn 81, Issue 3, 2006, Pages 476-480

  Wittebolle, Lieven ^a   Verstuyft, Karmen ^a   Verstraete, Willy ^a   Boon, Nico ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Flow cytometry; Hybridisation; Liquid array; Microsphere; Oligonucleotides; Probe

Indexed keywords

ADDITION REACTIONS; CHEMICAL ANALYSIS; COMPOSITION EFFECTS; FLOW OF FLUIDS; FLUORESCENCE; OLIGOMERS; PH EFFECTS;

AMINO-CARBOXY COUPLING; FLOW CYTOMETER; FLUORESCENCE INTENSITY; HYBRIDISATION; LIQUID ARRAYS; OLIGONUCLEOTIDES;

NUCLEIC ACIDS;

AMINO ACID; BORIC ACID; BUFFER; CARBOXYLIC ACID; MICROSPHERE; NUCLEIC ACID; OLIGONUCLEOTIDE; POLYSTYRENE;

ADDITION REACTIONS; CHEMICAL ANALYSIS; COMPOSITION EFFECTS; FLOW OF FLUIDS; FLUORESCENCE; NUCLEIC ACIDS; OLIGOMERS; PH EFFECTS;

ARTICLE; BIOTECHNOLOGY; CALIBRATION; CHEMICAL REACTION; CONCENTRATION (PARAMETERS); COVALENT BOND; DENSITY; DNA HYBRIDIZATION; DNA MICROARRAY; EUBACTERIUM; FLOW CYTOMETRY; FLUORESCENCE; INTERMETHOD COMPARISON; NONHUMAN; NUCLEIC ACID ANALYSIS; OLIGONUCLEOTIDE PROBE; PH; POLYMERASE CHAIN REACTION; PROCESS OPTIMIZATION; STEREOSPECIFICITY;

EID: 33644754862     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.1421     Document Type: Article

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