Lab assembly of a low-cost, robust SYBR green buffer system for quantitative real-time polymerase chain reaction

Analytical Biochemistry

Volumn 350, Issue 2, 2006, Pages 310-312

  Pellissier, François ^a   Glogowski, Carolina M ^b   Heinemann, Stephen F ^b   Ballivet, Marc ^a   Ossipow, Vincent ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COSTS;

BUFFER SYSTEM; LOW COSTS; QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION; SYBR GREEN;

POLYMERASE CHAIN REACTION;

BUFFER; DNA POLYMERASE; FLUORESCENT DYE; TAQ POLYMERASE;

ARTICLE; COST BENEFIT ANALYSIS; FINANCIAL MANAGEMENT; FLUORESCENCE ANALYSIS; GENE AMPLIFICATION; GENE TARGETING; LABORATORY APPARATUS, EQUIPMENT AND SUPPLIES; LABORATORY TEST; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION;

EID: 33644698789     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.12.002     Document Type: Article

References (3)

1. S. Giglio, P.T. Monis, and C.P. Saint Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR Nucleic Acids Res. 31 2003 136 140
2. A. Karsai, S. Muller, S. Platz, and M.T. Hauser Evaluation of a homemade SYBR Green I reaction mixture for real-time PCR quantification of gene expression Biotechniques 32 2002 790 796
3. V. Ossipow, F. Pellissier, O. Schaad, and M. Ballivet Gene expression analysis of the critical period in the visual cortex Mol. Cell. Neurosci. 27 2004 70 83