Collagen gel delivery of TGF-β3 non-viral plasmid DNA in rat osteoblast and calvarial culture

Orthodontics and Craniofacial Research

Volumn 8, Issue 4, 2005, Pages 320-322

  Premaraj, S ^a   Mundy, B ^a   Parker Barnes, J ^a   Winnard, P L ^a   Moursi, Amr M ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

Collagen gel; Cranial suture; Craniosynostosis; Nonviral plasmid delivery; Osteoblast; Tgf 3

Indexed keywords

BACTERIAL DNA; COLLAGEN; DRUG VEHICLE; GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN; RED FLUORESCENT PROTEIN; TGFB3 PROTEIN, RAT; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA3;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CULTURE TECHNIQUE; CYTOLOGY; GEL; GENETIC TRANSFECTION; GENETICS; METABOLISM; METHODOLOGY; OSTEOBLAST; PLASMID; RAT; SKULL; TISSUE ENGINEERING; VEHICLES;

ANIMALS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; COLLAGEN; DNA, BACTERIAL; GELS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; OSTEOBLASTS; PLASMIDS; RATS; SKULL; TISSUE ENGINEERING; TRANSFECTION; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA3; VEHICLES;

EID: 33644693358     PISSN: 16016335     EISSN: 16016343     Source Type: Journal    
DOI: 10.1111/j.1601-6343.2005.00355.x     Document Type: Article

References (4)

1. Chong SL, Mitchell R, Moursi AM, Winnard PL, Losken HW, Bradley J et al. Rescue of coronal suture fusion using transforming growth factor-beta 3 (Tgf-beta3) in rabbits with delayed-onset craniosynostosis. Anat Rec 2003;274A:962-71.
2. Moursi AM, Winnard PL, Fryer D, Mooney MP. Delivery of transforming growth factor-beta2-perturbing antibody in a collagen vehicle inhibits cranial suture fusion in calvarial organ culture. Cleft Palate Craniofac J 2003;40:225-32.
3. Fang J, Zhu YY, Smiley E, Bonadio J, Rouleau JP, Goldstein SA et al. Stimulation of new bone formation by direct transfer of osteogenic plasmid genes. Proc Natl Acad Sci USA 1996;93: 5753-8.
4. Scherer F, Schillinger U, Putz U, Stemberger A, Plank C. Nonviral vector loaded collagen sponges for sustained gene delivery in vitro and in vivo. J Gene Med 2002;4:634-43.