Molecular-friendly histopathology: Validation of a robust platform

Journal of Surgical Oncology

Volumn 93, Issue 4, 2006, Pages 231-234

  Nadji, Mehrdad ^a,b  

^a JACKSON MEMORIAL HOSPITAL   (United States)

^b Holtz Bldg 2147   (United States)

Author keywords

Alcohol fixatives; Histopathology; Microwave processing; Molecular diagnostics; Pharmacodiagnostics

Indexed keywords

BIOLOGICAL MARKER; FIXATIVE; PARAFFIN;

ARTICLE; DIAGNOSTIC PROCEDURE; HISTOPATHOLOGY; HUMAN; MACROMOLECULE; MORPHOMETRICS; PHARMACOGENOMICS; PRIORITY JOURNAL; TISSUE FIXATION; VALIDATION PROCESS;

EID: 33644682898     PISSN: 00224790     EISSN: 10969098     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Ross JS: The impact of molecular diagnostic tests on patient outcomes. Clin Lab Med 19:815-831, 1999
2. Liotta L, Petricoin E: Molecular profiling of human cancer. Nat Rev Genet 1:48-56, 2000
3. Lakhani SR, Ashworth A: Microarray and histopathological analysis of tumors: the future and the past? Nat Rev Cancer 1:151-157, 2001
4. Morales AR, Essenfeld H, Essenfeld E, Duboue MC, Vincek V, Nadji M: Continuous-specimen-flow, high-throughput, 1-hour tissue processing. A system for rapid diagnostic tissue preparation. Arch Pathol Lab Med 126:583-590, 2002
5. Grizzle WE: The use of fixative in diagnostic pathology. J Histotechnol 24:151-152, 2001
6. Lamar Jones M: To fix, to harden, to preserve-fixation: a brief history. J Histotechnol 24:155-162, 2001
7. Masuda N, Ohnishi T, Kawamoto S, Monden M, Okubo K: Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology application for such samples. Nucleic Acid Res 27:4436-4443, 1999
8. Lewis F, Maughan NJ, Smith V, Hillan K, Quirke P: Unlocking the archive gene expression in paraffin-embedded tissue. J Pathol 195:66-71, 2001
9. Vincek V, Nassiri M, Nadji M, Morales AR: A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples. Lab Invest 83:1427-1435, 2003
10. Kok LP, Boon ME: Ultrarapid vacuum-microwave histoprocessing. Histochem J 27:412-419, 1995
11. Leong AS-Y: Microwave irradiation in histopathology. Pathol Annu 2:213-233, 1998
12. Morales AR, Nassiri M, Kanhoush R, Vincek V, Nadji M: Experience with an automated microwave-assisted rapid tissue processing method. Validation of histologic quality and impact on the timeliness of diagnostic surgical pathology. Am J Clin Pathol 121:528-536, 2004
13. Bostwick DC, Al Anneuf N, Choi C: Establishment of the formalin-free surgical pathology laboratory. Utility of an alcohol-based fixative. Arch Pathol Lab Med 118:298-302, 1994
14. Nadji M, Nassiri M, Vincek V, Kanhoush R, Morales AR: Immunohistochemistry of tissue prepared by a molecular-friendly fixation and processing system. Appl Immunohistochem Mol Morphol 13:277-282, 2005
15. Mitchell D, Ibrahim S, Gasterson BA: Improved immunohistochemical localization of tissue antigens using Methacarn fixation. J Histochem Cytochem 33:491-495, 1985
16. Vincek V, Nassiri M, Block N, Welsh CF, Nadji M, Morales AR: Methodology for preservation of high molecular-weight RNA in paraffin-embedded tissue. Application for laser-capture microdissection. Diag Mol Pathol 14:127-133, 2005
17. Moskaluk CA: Tissue processing re-visited. A shifting paradigm in surgical pathology. Diag Mol Pathol 14:125-126, 2005