Design and synthesis of a chemically cleavable fluorescent nucleotide, 3′-O-Allyl-dGTP-allyl-Bodipy-FL-510, as a reversible terminator for DNA sequencing by synthesis

Journal of the American Chemical Society

Volumn 128, Issue 8, 2006, Pages 2542-2543

  Bi, Lanrong ^a,b   Kim, Dae Hyun ^a,b   Ju, Jingyue ^a,b  

^a Department of Neurology and the Taub Institute for Research on Alzheimer's Disease and the Aging Brain   (United States)

^b Fu Foundation School of Engineering and Applied Science   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLYL ALCOHOL; ALLYL COMPOUND; DEOXYGUANOSINE TRIPHOSPHATE; DISULFIDE; DNA; DNA POLYMERASE; FLUORESCENT DYE; NUCLEOTIDE DERIVATIVE;

ALLYLATION; ARTICLE; DNA SEQUENCE; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; PHOTOCHEMISTRY; SYNTHESIS;

ALLYL COMPOUNDS; BORON COMPOUNDS; DEOXYGUANINE NUCLEOTIDES; DNA; FLUORESCENT DYES; SEQUENCE ANALYSIS, DNA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 33644662470     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja057136n     Document Type: Article

References (8)

1. Collins, F. S.; Green, E. D.; Guttmacher, A. E.; Guyer, M. S. Nature 2003, 422, 835-847.
2. Ju, J.; Li, Z.; Edwards, J.; Itagaki, Y. U.S. Patent 6,664,079, 2003.
3. Meng, Q.; Kim, D. H.; Bai, X.; Bi, L.; Turro, N. J.; Ju, J. J. Am. Chem. Soc. Submitted
4. Ruparel, H.; Li, Z.; Bai, X.; Kim, D. H.; Turro, N. J.; Ju, J. Proc. Natl. Acad. Sci. U.S.A. 2005, 102, 5932-5937.
5. Mitra, R. D.; Shendure, J.; Olejnik, J,; Olejnik, E. K.; Church, G. M. Anal. Biochem. 2003, 320, 55-65.
6. Pleasants, J. C.; Guo, W.; Rabenstein, D. L. J. Am. Chem. Soc. 1989, 111, 6553-6558.
7. Huyghues-Despointes, B. M. P.; Nelson, J. W. Biochemistry 1992, 31, 1476-1483.
8. See the Supporting Information for experimental procedures and characterization data for all the compounds in Schemes 1 and 2.