The uses of green fluorescent protein in mammalian cells

Methods of Biochemical Analysis

Volumn 47, Issue , 2005, Pages 305-337

  Ward, Theresa H ^a   Lippincott Schwartz, Jennifer ^b  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN; RECOMBINANT PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL; ARTICLE; GENETIC TRANSFECTION; GENETIC VARIABILITY; GENETICS; MAMMAL; METHODOLOGY; REPORTER GENE;

AMINO ACID SUBSTITUTION; ANIMALS; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MAMMALS; RECOMBINANT PROTEINS; TRANSFECTION; VARIATION (GENETICS);

FLUORESCENCE MICROSCOPY; MAMMALS; REFRACTIVE INDEX;

GFP AND GFP VARIANTS; GREEN FLUORESCENT PROTEIN; MAMMALIAN CELLS; TIME-LAPSE IMAGING; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY;

FLUORESCENCE;

EID: 33644613731     PISSN: 00766941     EISSN: None     Source Type: Book Series    
DOI: 10.1002/0471739499.ch14     Document Type: Article

References (226)

1. Altan-Bonnet, N., Phair, R. D., Polishchuk, R. S., Weigert, R., and Lippincott-Schwartz, J. (2003). A role for Arf1 in mitotic Golgi disassembly, chromosome segregation, and cytokinesis. Proc. Natl. Acad. Sci. USA 100:13314-13319.
2. Anderson, M. T., Tjioe, I. M., Lorincz, M. C., Parks, D. R., Herzenberg, L. A., Nolan, G. P., and Herzenberg, L. A. (1996). Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins. Proc. Natl. Acad. Sci. USA 93:8508-8511.
3. Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H., and Miyawaki, A. (2002). An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. USA 99:12651-12656.
4. Axelrod, D. (2001). Total internal reflection fluorescence microscopy in cell biology. Traffic 2:764-774.
5. Bacia, K., Majoul, I. V., and Schwille, P. (2002). Probing the endocytic pathway in live cells using dual-color fluorescence cross-correlation analysis. Biophys. J. 83:1184-1193.
6. Bacskai, B. J., Hickey, G. A., Skoch, J., Kajdasz, S. T., Wang, Y., Huang, G.-F., Mathis, C. A., Klunk, W. E., and Hyman, B. T. (2003). Four-dimensional multiphoton imaging of brain entry, amyloid binding, and clearance of an amyloid-β ligand in transgenic mice. Proc. Natl. Acad. Sci. USA 100:12462-12467.
7. Bartlett, R. J., Singer, J. T., Shean, M. K., Weindler, F. W., and Secore, S. L. (1995). Expression of a fluorescent reporter gene in skeletal muscle cells from a virus-based vector: Implications for diabetes treatment. Transplant. Proc. 27:3344.
8. Bastiaens, P. I. H., and Squire, A. (1999). Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell. Trends Cell Biol. 9:48-52.
9. Beaudouin, J., Gerlich, D., Daigle, N., Eils, R., and Ellenberg, J. (2002). Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina. Cell 108:83-96.
10. Becker, M., Baumann, C., John, S., Walker, D. A., Vigneron, M., McNally, J. G., and Hager, G. L. (2002). Dynamic behavior of transcription factors on a natural promoter in living cells. EMBO Rep. 3:1188-1194.
11. Benmerah, A., Bayrou, M., Cerf-Bensussan, N., and Dautry-Varsat, A. (1999). Inhibition of clathrin-coated pit assembly by an Eps15 mutant. J. Cell Sci. 112:1303-1311.
12. Bergeland, T., Widerberg, J., Bakke, O., and Nordeng, T. W. (2001). Mitotic partitioning of endosomes and lysosomes. Curr. Biol. 11:644-651.
13. Bergsma, C. B. J., Streekstra, G. J., Smeulders, A. W. M., and Manders, E. M. M. (2001). Velocity estimation of spots in three-dimensional confocal image sequences of living cells. Cytometry 43:261-272.
14. Bevis, B. J., and Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nat. Biotechnol. 20:83-87.
15. Blum, R., Stephens, D. J., and Schulz, I. (2000). Lumenal targeted GFP, used as a marker of soluble cargo, visualizes rapid ERGIC to Golgi traffic by a tubulo-vesicular network. J. Cell Sci. 113:3151-3159.
16. Bonifacino, J. S., and Lippincott-Schwartz, J. (2003). Coat proteins: shaping membrane transport. Nat. Rev. Mol. Cell Biol. 4:409-414.
17. Bubulya, P. A., and Spector, D. L. (2004). "On the movements of nuclear components in living cells." Exp. Cell Res. 296:4-11.
18. Burke, B., and Ellenberg, J. (2002). Remodelling the walls of the nucleus. Nat. Rev. Mol. Cell Biol. 3:487-497.
19. Calleja, V., Ameer-Beg, S. M., Vojnovic, B., Woscholski, R., Downward, J., and Larijani, B. (2003). Monitoring conformational changes of proteins in cells by fluorescence lifetime imaging microscopy. Biochem. J. 372:33-40.
20. Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., and Tsien, R. Y. (2002). A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. USA 99:7877-7882.
21. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., and Prasher, D. C. (1994). Green fluorescent protein as a marker for gene expression. Science 263:802-805.
22. Chao, D. S., Hay, J. C., Winnick, S., Prekeris, R., Klumperman, J., and Scheller, R. H. (1999). SNARE membrane trafficking dynamics in vivo. J. Cell Biol. 144:869-881.
23. Chen, J., and Ahlquist, P. (2000). Brome mosaic virus polymerase-like protein 2a is directed to the endoplasmic reticulum by helicase-like viral protein 1a. J. Virol. 74:4310-4318.
24. Chiu, W.-L., Niwa, Y., Zeng, W., Hirano, T., Kobayashi, H., and Sheen, J. (1996). Engineered GFP as a vital reporter in plants. Curr. Biol. 6:325-330.
25. Choy, E., Chiu, V. K., Silletti, J., Feoktistov, M., Morimoto, T., Michaelson, D., Ivanov, I. E., and Philips, M. R. (1999). Endomembrane trafficking of Ras: The CAAX motif targets proteins to the ER and Golgi. Cell 98:69-80.
26. Christensen, M. O., Larsen, M. K., Barthelmes, H. U., Hock, R., Andersen, C. L., Kjeldsen, E., Knudsen, B. R., Westergaard, O., Boege, F., and Mielke, C. (2002). Dynamics of human DNA topoisomerases IIα and IIβ in living cells. J. Cell Biol. 157:31-44.
27. Chubb, J. R., Boyle, S., Perry, P., and Bickmore, W. A. (2002). Chromatin motion is constrained by association with nuclear compartments in human cells. Curr. Biol. 12:439-445.
28. Chudakov, D. M., Belousov, V. V., Zaraisky, A. G., Novoselov, V. V., Staroverov, D. B., Zorov, D. B., Lukyanov, S., and Lukyanov, K. A. (2003). Kindling fluorescent proteins for precise in vivo photolabeling. Nat. Biotechnol. 21:191-194.
29. Clegg, R. M. (1995). Fluorescence resonance energy transfer. Curr. Opin. Biotechnol. 6:103-110.
30. Cole, N. B., Smith, C. L., Sciaky, N., Terasaki, M., Edidin, M., and Lippincott-Schwartz, J. (1996). Diffusional mobility of Golgi proteins in membranes of living cells. Science 273: 797-801.
31. Conrad, C., Erfle, H., Warnat, P., Daigle, N., Lörch, T., Ellenberg, J., Pepperkok, R., and Eils, R. (2004). Automatic identification of subcellular phenotypes on human cell arrays. Genome Res. 14:1130-1136.
32. Contag, P. R., Olomu, I. N., Stevenson, D. K., and Contag, C. H. (1998). Bioluminescent indicators in living mammals. Nat. Med. 4:245-247.
33. Cook, L. B., and Hinkle, P. M. (2004). Fate of internalized thyrotropin-releasing hormone receptors monitored with a timer fusion protein. Endocrinology 145:3095-3100.
34. Cook, S. H., and Griffin, D. E. (2003). Luciferase imaging of a neurotropic viral infection in intact animals. J. Virol. 77:5333-5338.
35. Cormack, B. P., Valdivia, R. H., and Falkow, S. (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38.
36. Crameri, A., Whitehorn, E. A., Tate, E., and Stemmer, W. P. (1996). Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat. Biotechnol. 14:315-319.
37. Cukierman, E., Pankov, R., Stevens, D. R., and Yamada, K. M. (2001). Taking cell-matrix adhesions to the third dimension. Science 294:1708-1712.
38. Dahm, T., White, J., Grill, S., Füllekrug, J., and Stelzer, E. H. K. (2001). Quantitative ER ↔ Golgi transport kinetics and protein separation upon Golgi exit revealed by vesicular integral membrane protein 36 dynamics in live cells. Mol. Biol. Cell 12:1481-1498.
39. Daigle, N., Beaudouin, J., Hartnell, L., Imreh, G., Hallberg, E., Lippincott-Schwartz, J., and Ellenberg, J. (2001). Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells. J. Cell Biol. 154:71-84.
40. Delagrave, S., Hawtin, R. E., Silva, C. M., Yang, M. M., and Youvan, D. C. (1995). Red-shifted excitation mutants of the green fluorescent protein. Biotechnol. 13:151-154.
41. Dhandayuthapani, S., Via, L. E., Thomas, C. A., Horowitz, P. M., Deretic, D., and Deretic, V. (1995). Green fluorescent protein as a marker for gene expression and cell biology of mycobacterial interactions with macrophages. Mol. Microbiol. 17:901-912.
42. Dietzel, S., and Belmont, A. S. (2001). Reproducible but dynamic positioning of DNA in chromosomes during mitosis. Nat. Cell Biol. 3:767-770.
43. Dorsky, D. I., Wells, M., and Harrington, R. D. (1996). Detection of HIV-1 infection with a green fluorescent protein reporter system. J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. 13:308-313.
44. Dundr, M., McNally, J. G., Cohen, J., and Misteli, T. (2002). Quantitation of GFP-fusion proteins in single living cells. J. Struct. Biol. 140:92-99.
45. Dundr, M., Hebert, M. D., Karpova, T. S., Stanek, D., Xu, H., Shpargel, K. B., Meier, U. T., Neugebauer, K. M., Matera, A. G., and Misteli, T. (2004). In vivo kinetics of Cajal body components. J. Cell Biol. 164:831-842.
46. Dunn, G. A., Dobbie, I. M., Monypenny, J., Holt, M. R., and Zicha, D. (2002). Fluorescence localization after photobleaching (FLAP): a new method for studying protein dynamics in living cells. J. Microsc. 205:109-112.
47. Dyba, M., Jakobs, S., and Hell, S. W. (2003). Immunofluorescence stimulated emission depletion microscopy. Nat. Biotechnol. 21:1303-1304.
48. Edidin, M. (1994). Fluorescence photobleaching and recovery, FPR, in the analysis of membrane structure and dynamics. In Mobility and Proximity in Biological Membranes, Damjanovich, S., Edidin, M., Szollosi, J., and Tron, L., Eds., CRC, Boca Raton, FL, pp. 109-135.
49. Ehrig, T., O'Kane, D. J., and Prendergast, F. G. (1995). Green-fluorescent protein mutants with altered fluorescence excitation spectra. FEBS Lett. 367:163-166.
50. Eils, R., Gerlich, D., Tvaruskó, W., Spector, D. L., and Misteli, T. (2000). Quantitative imaging of pre-mRNA splicing factors in living cells. Mol. Biol. Cell 11:413-418.
51. Ellenberg, J., Siggia, E. D., Moreira, J. E., Smith, C. L., Presley, J. F., Worman, H. J., and Lippincott-Schwartz, J. (1997). Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis. J. Cell Biol. 138:1193-1206.
52. Ellenberg, J., Lippincott-Schwartz, J., and Presley, J. F. (1998). Two-color green fluorescent protein time-lapse imaging. Biotechniques 25:838-846.
53. Elliott, G., and O'Hare, P. (1999). Live-cell analysis of a green fluorescent protein-tagged herpes simplex virus infection. J. Virol. 73:4110-4119.
54. Elowitz, M. B., Surette, M. G., Wolf, P. E., Stock, J., and Leibler, S. (1997). Photoactivation turns green fluorescent protein red. Curr. Biol. 7:809-812.
55. Elsner, M., Hashimoto, H., Simpson, J. C., Cassel, D., Nilsson, T., and Weiss, M. (2003). Spatiotemporal dynamics of the COPI vesicle machinery. EMBO Rep. 4:1000-1004.
56. Engqvist-Goldstein, A. E. Y., Zhang, C. X., Carreno, S., Barroso, C., Heuser, J. E., and Drubin, D. G. (2004). RNAi-mediated Hip1R silencing results in stable association between the endocytic machinery and the actin assembly machinery. Mol. Biol. Cell 15:1666-1679.
57. Espinet, C., Gómez-Arbonés, X., Egea, J., and Comella, J. X. (2000). Combined use of the green and yellow fluorescent proteins and fluorescence-activated cell sorting to select populations of transiently transfected PC12 cells. J. Neurosci. Methods 100:63-69.
58. Essers, J., Houtsmuller, A. B., van Veelen, L., Paulusma, C., Nigg, A. L., Pastink, A., Vermeulen, W., Hoeijmakers, J. H. J., and Kanaar, R. (2002). Nuclear dynamics of RAD52 group homologous recombination proteins in response to DNA damage. EMBO J. 21:2030-2037.
59. Feng, Y., Yu, S., Lasell, T. K. R., Jadhav, A. P., Macia, E., Chardin, P., Melancon, P., Roth, M., Mitchison, T., and Kirchhausen, T. (2003). Exo1: A new chemical inhibitor of the exocytic pathway. Proc. Natl. Acad. Sci. USA 100:6469-6474.
60. Fradin, C., Abu-Arish, A., Granek, R., and Elbaum, M. (2003). Fluorescence correlation spectroscopy close to a fluctuating membrane. Biophys. J. 84:2005-2020.
61. Gaidarov, I., Santini, F., Warren, R. A., and Keen, J. H. (1999). Spatial control of coated-pit dynamics in living cells. Nat. Cell Biol. 1:1-7.
62. García-Mata, R., Szul, T., Alvarez, C., and Sztul, E. (2003). ADP-ribosylation factor/COPI-dependent events at the endoplasmic reticulum-Golgi interface are regulated by the guanine nucleotide exchange factor GBF1. Mol. Biol. Cell 14:2250-2261.
63. Gerlich, D., Beaudouin, J., Gebhard, M., Ellenberg, J., and Eils, R. (2001). Four-dimensional imaging and quantitative reconstruction to analyse complex spatiotemporal processes in live cells. Nat. Cell Biol. 3:852-855.
64. Gerlich, D., Beaudouin, J., Kalbfuss, B., Daigle, N., Eils, R., and Ellenberg, J. (2003). Global chromosome positions are transmitted through mitosis in mammalian cells. Cell 112:751-764.
65. Gillham, H., Golding, M. C. H. M., Pepperkok, R., and Gullick, W. J. (1999). Intracellular movement of green fluorescent protein-tagged phosphatidylinositol 3-kinase in response to growth factor receptor signaling. J. Cell Biol. 146:869-880.
66. Glebov, O. O., and Nichols, B. J. (2004). Lipid raft proteins have a random distribution during localized activation of the T-cell receptor. Nat. Cell Biol. 6:238-243.
67. Griesbeck, O., Baird, G. S., Campbell, R. E., Zacharias, D. A., and Tsien, R. Y. (2001). Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. J. Biol. Chem. 276:29188-29194.
68. Griffis, E. R., Altan, N., Lippincott-Schwartz, J., and Powers, M. A. (2002). Nup98 is a mobile nucleoporin with transcription-dependent dynamics. Mol. Biol. Cell 13:1282-1297.
69. Gurskaya, N. G., Fradkov, A. F., Terskikh, A., Matz, M. V., Labas, Y. A., Martynov, V. I., Yanushevich, Y. G., Lukyanov, K. A., and Lukyanov, S. A. (2001). GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Lett. 507:16-20.
70. Haraguchi, T., Ding, D. Q., Yamamoto, A., Kaneda, T., Koujin, T., and Hiraoka, Y. (1999). Multiple-color fluorescence imaging of chromosomes and microtubules in living cells. Cell Struct. Funct. 24:291-298.
71. Haraguchi, T., Koujin, T., Hayakawa, T., Kaneda, T., Tsutsumi, C., Imamoto, N., Akazawa, C., Sukegawa, J., Yoneda, Y., and Hiraoka, Y. (2000). Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes. J. Cell Sci. 113:779-794.
72. 2+ channels in live cells. Biophys. J. 81:2639-2646.
73. Hayes, N., Howard-Cofield, E., and Gullick, W. (2004). Green fluorescent protein as a tool to study epidermal growth factor receptor function. Cancer Lett. 206:129-135.
74. Hegerfeldt, Y., Tusch, M., Bröcker, E.-B., and Friedl, P. (2002). Collective cell movement in primary melanoma explants: Plasticity of cell-cell interaction, β1-integrin function, and migration strategies. Cancer Res. 62:2125-2130.
75. Heim, R., and Tsien, R. Y. (1996). Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6:178-182.
76. Heim, R., Prasher, D. C., and Tsien, R. Y. (1994). Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA 91:12501-12504.
77. Heim, R., Cubitt, A. B., and Tsien, R. Y. (1995). Improved green fluorescence. Nature 373:663-664.
78. Hirschberg, K., Miller, C. M., Ellenberg, J., Presley, J. F., Siggia, E. D., Phair, R. D., and Lippincott-Schwartz, J. (1998). Kinetic analysis of secretory protein traffic and characterization of Golgi to plasma membrane transport intermediates in living cells. J. Cell Biol. 143:1485-1503.
79. Holaska, J. M., Wilson, K. L., and Mansharamani, M. (2002). The nuclear envelope, lamins and nuclear assembly. Curr. Opin. Cell Biol. 14:357-364.
80. Houtsmuller, A. B., Rademakers, S., Nigg, A. L., Hoogstraten, D., Hoeijmakers, J. H. J., and Vermeulen, W. (1999). Action of DNA repair endonuclease ERCC1/XPF in living cells. Science 284:958-961.
81. Hume, A. N., Collinson, L. M., Rapak, A., Gomes, A. Q., Hopkins, C. R., and Seabra, M. C. (2001). Rab27a regulates the peripheral distribution of melanosomes in melanocytes. J. Cell Biol. 152:795-808.
82. Inouye, S., and Tsuji, F. I. (1994). Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett. 341:277-280.
83. Janicki, S. M., and Spector, D. L. (2003). Nuclear choreography: Interpretations from living cells. Curr. Opin. Cell Biol. 15:149-157.
84. Jayaraman, S., Haggie, P., Wachter, R. M., Remington, S. J., and Verkman, A. S. (2000). Mechanism and cellular applications of a green fluorescent protein-based halide sensor. J. Biol. Chem. 275:6047-6050.
85. Jokitalo, E., Cabrera-Poch, N., Warren, G., and Shima, D. T. (2001). Golgi clusters and vesicles mediate mitotic inheritance independently of the endoplasmic reticulum. J. Cell Biol. 154:317-330.
86. Kaether, C., and Gerdes, H.-H. (1995). Visualization of protein transport along the secretory pathway using green fluorescent protein. FEBS Lett. 369:267-271.
87. Keller, P., Toomre, D., Diaz, E., White, J., and Simons, K. (2001). Multicolour imaging of post-Golgi sorting and trafficking in live cells. Nat. Cell Biol. 3:140-149.
88. Kenworthy, A. (2002). Peering inside lipid rafts and caveolae. Trends Biochem. Sci. 27:435-437.
89. Kenworthy, A. K., Nichols, B. J., Remmert, C. L., Hendrix, G. M., Kumar, M., Zimmerberg, J., and Lippincott-Schwartz, J. (2004). Dynamics of putative raft-associated proteins at the cell surface. J. Cell Biol. 165:735-746.
90. Kimura, H., and Cook, P. R. (2001). Kinetics of core histones in living human cells: Little exchange of H3 and H4 and some rapid exchange of H2B. J. Cell Biol. 153:1341-1353.
91. Kimura, H., Sugaya, K., and Cook, P. R. (2002). The transcription cycle of RNA polymerase II in living cells. J. Cell Biol. 159:777-782.
92. Kirchner, J., Kam, Z., Tzur, G., Bershadsky, A. D., and Geiger, B. (2003). Live-cell monitoring of tyrosine phosphorylation in focal adhesions following microtubule disruption. J. Cell Sci. 116:975-986.
93. Kreitzer, G., Marmorstein, A., Okamoto, P., Vallee, R., and Rodriguez-Boulan, E. (2000). Kinesin and dynamin are required for post-Golgi transport of a plasma-membrane protein. Nat. Cell Biol. 2:125-127.
94. Kreitzer, G., Schmoranzer, J., Low, S. H., Li, X., Gan, Y., Weimbs, T., Simon, S. M., and Rodriguez-Boulan, E. (2003). Three-dimensional analysis of post-Golgi carrier exocytosis in epithelial cells. Nat. Cell Biol. 5:126-136.
95. Krichevsky, O., and Bonnet, G. (2002). Fluorescence correlation spectroscopy: the technique and its applications. Rep. Prog. Phys. 65:251-297.
96. Krylyshkina, O., Anderson, K. I., Kaverina, I., Upmann, I., Manstein, D. J., Small, J. V., and Toomre, D. K. (2003). Nanometer targeting of microtubules to focal adhesions. J. Cell Biol. 161:853-859.
97. La Boissière, S., Izeta, A., Malcomber, S., and O'Hare, P. (2004). Compartmentalization of VP16 in cells infected with recombinant herpes simplex virus expressing VP16-green fluorescent protein fusion proteins. J. Virol. 78:8002-8014.
98. Lakowicz, J. R., Szmacinski, H., Nowaczyk, K., Berndt, K. W., and Johnson, M. (1992). Fluorescence lifetime imaging. Anal. Biochem. 202:316-330.
99. Lamond, A. I., and Spector, D. L. (2003). Nuclear speckles: a model for nuclear organelles. Nat. Rev. Mol. Cell Biol. 4:605-612.
100. Larson, D. R., Ma, Y. M., Vogt, V. M., and Webb, W. W. (2003). Direct measurement of Gag-Gag interaction during retrovirus assembly with FRET and fluorescence correlation spectroscopy. J. Cell Biol. 162:1233-1244.
101. Lever, M. A., Th'ng, J. P., Sun, X., and Hendzel, M. J. (2000). Rapid exchange of histone H1.1 on chromatin in living human cells. Nature 408:873-876.
102. Lin, H.-J., Herman, P., and Lakowicz, J. R. (2003). Fluorescence lifetime-resolved pH imaging of living cells. Cytometry 52A:77-89.
103. Lippincott-Schwartz, J., Snapp, E., and Kenworthy, A. (2001). Studying protein dynamics in living cells. Nat. Rev. Mol. Cell Biol. 2:444-456.
104. Ludin, B., and Matus, A. (1998). GFP illuminates the cytoskeleton. Trends Cell Biol. 8:72-77.
105. Mahajan, N. P., Harrison-Shostak, D. C., Michaux, J., and Herman, B. (1999). Novel mutant green fluorescent protein protease substrates reveal the activation of specific caspases during apoptosis. Chem. Biol. 6:401-409.
106. Majoul, I., Straub, M., Hell, S. W., Duden, R., and Söling, H. D. (2001). KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: Measurements in living cells using FRET. Dev. Cell 1:139-153.
107. Mancia, F., Patel, S. D., Rajala, M. W., Scherer, P. E., Nemes, A., Schieren, I., Hendrickson, W. A., and Shapiro, L. (2004). Optimization of protein production in mammalian cells with a coexpressed fluorescent marker. Structure 12:1355-1360.
108. Manneville, J.-B., Etienne-Manneville, S., Skehel, P., Carter, T., Ogden, D., and Ferenczi, M. (2003). Interaction of the actin cytoskeleton with microtubules regulates secretory organelle movement near the plasma membrane in human endothelial cells. J. Cell Sci. 116:3927-3938.
109. Marshall, J., Molloy, R., Moss, G., Howe, J. R., and Hughes, T. E. (1995). The jellyfish green fluorescent protein: A new tool for studying ion channel expression. Neuron 14:211-215.
110. Mayor, S., Presley, J. F., and Maxfield, F. R. (1993). Sorting of membrane components from endosomes and subsequent recycling to the cell surface occurs by a bulk flow process. J. Cell Biol. 121:1257-1269.
111. McNally, J. G., Müller, W. G., Walker, D., Wolford, R., and Hager, G. L. (2000). The glucocorticoid receptor: Rapid exchange with regulatory sites in living cells. Science 287:1262-1265.
112. Mempel, T. R., Henrickson, S. E., and von Andrian, U. H. (2004). T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases. Nature 427:154-159.
113. Merrifield, C. J., Feldman, M. E., Wan, L., and Almers, W. (2002). Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits. Nat. Cell Biol. 4:691-698.
114. Meulenbroek, R. A., Sargent, K. L., Lunde, J., Jasmin, B. J., and Parks, R. J. (2004). Use of adenovirus protein IX (pIX) to display large polypeptides on the virion - Generation of fluorescent virus through the incorporation of pIX-GFP. Mol. Ther. 9:617-624.
115. Miesenböck, G., De Angelis, D. A., and Rothman, J. E. (1998). Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394:192-195.
116. Miles, S., McManus, H., Forsten, K. E., and Storrie, B. (2001). Evidence that the entire Golgi apparatus cycles in interphase HeLa cells: sensitivity of Golgi matrix proteins to an ER exit block. J. Cell Biol. 155:543-555.
117. Mironov, A. A., Mironov, A. A., Jr., Beznoussenko, G. V., Trucco, A., Lupetti, P., Smith, J. D., Geerts, W. J. C., Koster, A. J., Burger, K. N. J., Martone, M. E., Deerinck, T. J., Ellisman, M. H., and Luini, A. (2003). ER-to-Golgi carriers arise through direct en bloc protrusion and multistage maturation of specialized ER exit domains. Dev. Cell 5:583-594.
118. Misteli, T. (2001). The concept of self-organization in cellular architecture. J. Cell Biol. 155:181-185.
119. Misteli, T., Gunjan, A., Hock, R., Bustin, M., and Brown, D. T. (2000). Dynamic binding of histone H1 to chromatin in living cells. Nature 408:877-881.
120. Mitra, R. D., Silva, C. M., and Youvan, D. C. (1996). Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein. Gene 173:13-17.
121. 2+ based on green fluorescent proteins and calmodulin. Nature 388:882-887.
122. Moir, R. D., Yoon, M., Khuon, S., and Goldman, R. D. (2000). Nuclear lamins A and B1: Different pathways of assembly during nuclear envelope formation in living cells. J. Cell Biol. 151:1155-1168.
123. Moradpour, D., Evans, M. J., Gosert, R., Yuan, Z., Blum, H. E., Goff, S. P., Lindenbach, B. D., and Rice, C. M. (2004). Insertion of green fluorescent protein into non-structural protein 5A allows direct visualization of functional hepatitis C virus replication complexes. J. Virol. 78:7400-7409.
124. Moriyoshi, K., Richards, L. J., Akazawa, C., O'Leary, D. D., and Nakanishi, S. (1996). Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP. Neuron 16:255-260.
125. Mosser, D. D., Caron, A. W., Bourget, L., Jolicoeur, P., and Massie, B. (1997). Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products. Biotechniques 22:150-154.
126. Mundy, D. I., Machleidt, T., Ying, Y. S., Anderson, R. G., and Bloom, G. S. (2002). Dual control of caveolar membrane traffic by microtubules and the actin cytoskeleton. J. Cell Sci. 115:4327-4339.
127. Munro, S. (2003). Lipid rafts: elusive or illusive? Cell 115:377-388.
128. Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002). A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20:87-90.
129. Nakata, T., and Hirokawa, N. (2003). Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head. J. Cell Biol. 162:1045-1055.
130. Nehls, S., Snapp, E. L., Cole, N. B., Zaal, K. J., Kenworthy, A. K., Roberts, T. H., Ellenberg, J., Presley, J. F., Siggia, E., and Lippincott-Schwartz, J. (2000). Dynamics and retention of misfolded proteins in native ER membranes. Nat. Cell Biol. 2:288-295.
131. Ng, T., Squire, A., Hansra, G., Bornancin, P., Prevostel, C., Hanby, A., Harris, W., Barnes, D., Schmidt, S., Mellor, H., Bastiaens, P. I., and Parker, P. J. (1999). Imaging protein kinase Cα activation in cells. Science 283:2085-2089.
132. Nichols, B. J. (2002). A distinct class of endosome mediates clathrin-independent endocytosis to the Golgi complex. Nat. Cell Biol. 4:374-378.
133. Nichols, B. J., Kenworthy, A. K., Polishchuk, R. S., Lodge, R., Roberts, T. H., Hirschberg, K., Phair, R. D., and Lippincott-Schwartz, J. (2001). Rapid cycling of lipid raft markers between the cell surface and Golgi complex. J. Cell Biol. 153:529-541.
134. Niswender, K. D., Blackman, S. M., Rohde, L., Magnuson, M. A., and Piston, D. W. (1995). Quantitative imaging of green fluorescent protein in cultured cells: Comparison of microscopic techniques, use in fusion proteins and detection limits. J. Microsc. 180:109-116.
135. Ogawa, H., Inouye, S., Tsuji, F. I., Yasuda, K., and Umesono, K. (1995). Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells. Proc. Natl. Acad. Sci. USA 92:11899-11903.
136. Olson, K. R., McIntosh, J. R., and Olmsted, J. B. (1995). Analysis of MAP 4 function in living cells using green fluorescent protein (GFP) chimeras. J. Cell Biol. 130:639-650.
137. Ormö, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., and Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science 273:1392-1395.
138. Ostlund, C., Ellenberg, J., Hallberg, E., Lippincott-Schwartz, J., and Worman, H. J. (1999). Intracellular trafficking of emerin, the Emery-Dreifuss muscular dystrophy protein. J. Cell Sci. 112:1709-1719.
139. Patterson, G. H., and Lippincott-Schwartz, J. (2002). A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297:1873-1877.
140. Peel, A. L., Zolotukhin, S., Schrimsher, G. W., Muzyczka, N., and Reier, P. J. (1997). Efficient transduction of green fluorescent protein in spinal cord neurons using adeno-associated virus vectors containing cell type-specific promoters. Gene Ther. 4:16-24.
141. Pelkmans, L., Püntener, D., and Helenius, A. (2002). Local actin polymerization and dynamin recruitment in SV40-induced internalization of caveolae. Science 296:535-539.
142. Pepperkok, R., Squire, A., Geley, S., and Bastiaens, P. I. (1999). Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy. Curr. Biol. 9:269-272.
143. Petersen, N. O., Hoddelius, P. L., Wiseman, P. W., Seger, O., and Magnusson, K. E. (1993). Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application. Biophys. J. 65:1135-1146.
144. Petroll, W. M., Ma, L., and Jester, J. V. (2003). Direct correlation of collagen matrix deformation with focal adhesion dynamics in living corneal fibroblasts. J. Cell Sci. 116:1481-1491.
145. Phair, R. D., and Misteli, T. (2000). High mobility of proteins in the mammalian cell nucleus. Nature 404:604-609.
146. Piston, D. W. (1999). Imaging living cells and tissues by two-photon excitation microscopy. Trends Cell Biol. 9:66-69.
147. Polishchuk, R. S., Polishchuk, E. V., Marra, P., Alberti, S., Buccione, R., Luini, A., and Mironov, A. A. (2000). Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between Golgi apparatus and plasma membrane. J. Cell Biol. 148:45-58.
148. Polishchuk, E. V., Di Pentima, A., Luini, A., and Polishchuk, R. S. (2003). Mechanism of constitutive export from the Golgi: bulk flow via the formation, protrusion, and en bloc cleavage of large trans-Golgi network tubular domains. Mol. Biol. Cell 14:4470-4485.
149. Polishchuk, R., Di Pentima, A., and Lippincott-Schwartz, J. (2004). Delivery of raft-associated, GPI-anchored proteins to the apical surface of polarized MDCK cells by a transcytotic pathway. Nat. Cell Biol. 6:297-307.
150. Pollok, B. A., and Heim, R. (1999). Using GFP in FRET-based applications. Trends Cell Biol. 9:57-60.
151. Potel., C., Kaelin, K., Gautier, I., Lebon, P., Coppey, J., and Rozenberg, F. (2002). Incorporation of green fluorescent protein into the essential envelope glycoprotein B of herpes simplex virus type 1. J. Virol. Methods 105:13-23.
152. Presley, J. F., Cole, N. B., Schroer, T. A., Hirschberg, K., Zaal, K. J. M., and Lippincott-Schwartz, J. (1997). ER-to-Golgi transport visualized in living cells. Nature 389:81-85.
153. Presley, J. F., Smith, C., Hirschberg, K., Miller, C., Cole, N. B., Zaal, K. J. M., and Lippincott-Schwartz, J. (1998). Golgi membrane dynamics. Mol. Biol. Cell 9:1617-1626.
154. Presley, J. F., Ward, T. H., Pfeifer, A. C., Siggia, E. D., Phair, R. D., and Lippincott-Schwartz, J. (2002). Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport. Nature 417:187-193.
155. Pyenta, P. S., Holowka, D., and Baird, B. (2001). Cross-correlation analysis of inner-leaflet-anchored green fluorescent protein co-redistributed with IgE receptors and outer leaflet lipid raft components. Biophys. J. 80:2120-2132.
156. Rizzo, M. A., Springer, G. H., Granada, B., and Piston, D. W. (2004). An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22:445-449.
157. Rizzuto, R., Brini, M., De Giorgi, F., Rossi, R., Heim, R., Tsien, R. Y., and Pozzan, T. (1996). Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo. Curr. Biol. 6:183-188.
158. Robbins, J. R., Barth, A. I., Marquis, H., de Hostos, E. L., Nelson, W. J., and Theriot, J. A. (1999). Listeria monocytogenes exploits normal host cell processes to spread from cell to cell. J. Cell Biol. 146:1333-1350.
159. Roberts, R. L., Barbieri, M. A., Pryse, K. M., Chua, M., Morisaki, J. H., and Stahl, P. D. (1999). Endosome fusion in living cells overexpressing GFP-rab5. J. Cell Sci. 112:3667-3675.
160. Rocheleau, J. V., Edidin, M., and Piston, D. W. (2003). Intrasequence GFP in class I MHC molecules, a rigid probe for fluorescence anisotropy measurements of the membrane environment. Biophys. J. 84:4078-4086.
161. Ropp, J. D., Donahue, C. J., Wolfgang-Kimball, D., Hooley, J. J., Chin, J. Y., Cuthbertson, R. A., and Bauer, K. D. (1996). Aequorea green fluorescent protein: simultaneous analysis of wild-type and blue-fluorescing mutant by flow cytometry. Cytometry 24:284-288.
162. Rudolf, R., Salm, T., Rustom, A., and Gerdes, H.-H. (2001). Dynamics of immature secretory granules: Role of cytoskeletal elements during transport, cortical restriction, and F-actin-dependent tethering. Mol. Biol. Cell 12:1353-1365.
163. Sako, Y., Minoghchi, S., and Yanagida, T. (2000). Single-molecule imaging of EGFR signalling on the surface of living cells. Nat. Cell Biol. 2:168-172.
164. Salina, D., Bodoor, K., Eckley, D. M., Schroer, T. A., Rattner, J. B., and Burke, B. (2002). Cytoplasmic dynein as a facilitator of nuclear envelope breakdown. Cell 108:97-107.
165. Salmon, W. C., Adams, M. C., and Waterman-Storer, C. M. (2002). Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells. J. Cell Biol. 158:31-37.
166. Sato, M., Ozawa, T., Inukai, K., Asano, T., and Umezawa, Y. (2002). Fluorescent indicators for imaging protein phosphorylation in single living cells. Nat. Biotechnol. 20:287-294.
167. Scales, S. J., Pepperkok, R., and Kreis, T. E. (1997). Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI. Cell 90:1137-1148.
168. Schmoranzer, J., and Simon, S. M. (2003). Role of microtubules in fusion of post-Golgi vesicles to the plasma membrane. Mol. Biol. Cell 14:1558-1569.
169. Schmoranzer, J., Goulian, M., Axelrod, D., and Simon, S. M. (2000). Imaging constitutive exocytosis with total internal reflection fluorescence microscopy. J. Cell Biol. 149:23-32.
170. Sciaky, N., Presley, J., Smith, C., Zaal, K. J. M., Cole, N., Moreira, J. E., Terasaki, M., Siggia, E., and Lippincott-Schwartz, J. (1997). Golgi tubule traffic and the effects of brefeldin A visualized in living cells. J. Cell Biol. 139:1137-1155.
171. Shav-Tal, Y., Darzacq, X., Shenoy, S. M., Fusco, D., Janicki, S. M., Spector, D. L., and Singer, R. H. (2004). Dynamics of single mRNPs in nuclei of living cells. Science 304:1797-1800.
172. Shima, D. T., Scales, S. J., Kreis, T. E., and Pepperkok, R. (1999). Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes. Curr. Biol. 9:821-824.
173. Shorter, J., and Warren, G. (2002). Golgi architecture and inheritance. Annu. Rev. Cell Dev. Biol. 18:379-420.
174. Simons, K., and Vaz, W. L. (2004). Model systems, lipid rafts, and cell membranes. Annu. Rev. Biophys. Biomol. Struct. 33:269-295.
175. Sineshchekova, O. O., Kawate, T., Vdovychenko, O. V., and Sato, T. N. (2004). Protein-trap version 2.1: screening for expressed proteins in mammalian cells based on their localizations. BMC Cell Biol. 5:8.
176. Smith, J., Yu, R., and Hinkle, P. M. (2001). Activation of MAPK by TRH requires clathrin-dependent endocytosis and PKC but not receptor interaction with β-arrestin or receptor endocytosis. Mol. Endocrinol. 15:1539-1548.
177. Snapp, E. L., Hegde, R. S., Francolini, M., Lombardo, F., Colombo, S., Pedrazzini, E., Borgese, N., and Lippincott-Schwartz, J. (2003). Formation of stacked ER cisternae by low affinity protein interactions. J. Cell Biol. 163:257-269.
178. Snapp, E. L., Reinhart, G. A., Bogert, B. A., Lippincott-Schwartz, J., and Hegde, R. S. (2004). The organization of engaged and quiescent translocons in the endoplasmic reticulum of mammalian cells. J. Cell Biol. 164:997-1007.
179. Sönnichsen, B., De Renzis, S., Nielsen, E., Rietdorf, J., and Zerial, M. (2000). Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11. J. Cell Biol. 149:901-914.
180. Stenoien, D. L., Nye, A. C., Mancini, M. G., Patel, K., Dutertre, M., O'Malley, B. W., Smith, C. L., Belmont, A. S., and Mancini, M. A. (2001). Ligand-mediated assembly and real-time cellular dynamics of estrogen receptor α-coactivator complexes in living cells. Mol. Cell. Biol. 21:4404-4412.
181. Stephens, D. J., and Pepperkok, R. (2002). Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells. J. Cell Sci. 115:1149-1160.
182. Stephens, D. J., and Pepperkok, R. (2004). Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum. J. Cell Sci. 117:3635-3644.
183. Stephens, D. J., Lin-Marq, N., Pagano, A., Pepperkok, R., and Paccaud, J. P. (2000). COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites. J. Cell Sci. 113:2177-2185.
184. Storrie, B., White, J., Röttger, S., Stelzer, E. H., Suganuma, T., and Nilsson, T. (1998). Recycling of Golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering. J. Cell Biol. 143:1505-1521.
185. Stryer, L. (1978). Fluorescence energy transfer as a spectroscopic ruler. Annu. Rev. Biochem. 47:819-846.
186. Sutherland, H. G., Mumford, G. K., Newton, K., Ford, L. V., Farrall, R., Dellaire, G., Caceres, J. F., and Bickmore, W. A. (2001). Large-scale identification of mammalian proteins localized to nuclear sub-compartments. Hum. Mol. Genet. 10:1995-2011.
187. Taylor, T. J., McNamee, E. E., Day, C., and Knipe, D. M. (2003). Herpes simplex virus replication compartments can form by coalescence of smaller compartments. Virol. 309:232-247.
188. Terasaki, M., Jaffe, L. A., Hunnicutt, G. R., and Hammer, J. A., III (1996). Structural change of the endoplasmic reticulum during fertilization: Evidence for loss of membrane continuity using green fluorescent protein. Dev. Biol. 179:320-328.
189. Terskikh, A., Fradkov, A., Ermakova, G., Zaraisky, A., Tan, P., Kajava, A. V., Zhao, X., Lukyanov, S., Matz, M., Kim, S., Weissman, I., and Siebert, P. (2000). "Fluorescent timer": Protein that changes color with time. Science 290:1585-1588.
190. Thomsen, P., Roepstorff, K., Stahlhut, M., and van Deurs, B. (2002). Caveolae are highly immobile plasma membrane microdomains, which are not involved in constitutive endocytic trafficking. Mol. Biol. Cell 13:238-250.
191. Toomre, D., and Manstein, D. J. (2001). Lighting up the cell surface with evanescent wave microscopy. Trends Cell Biol. 11:298-303.
192. Toomre, D., Keller, P., White, J., Olivo, J. C., and Simons, K. (1999). Dual-color visualization of trans-Golgi network to plasma membrane traffic along microtubules in living cells. J. Cell Sci. 112:21-33.
193. Toomre, D., Steyer, J. A., Keller, P., Almers, W., and Simons, K. (2000). Fusion of constitutive membrane traffic with the cell surface observed by evanescent wave microscopy. J. Cell Biol. 149:33-40.
194. Tsien, R. Y., Bacskai, B. J., and Adams, S. R. (1993). FRET for studying intracellular signalling. Trends Cell Biol. 3:242-245.
195. Tvaruskó, W., Bentele, M., Misteli, T., Rudolf, R., Kaether, C., Spector, D. L., Gerdes, H. H., and Eils, R. (1999). Time-resolved analysis and visualization of dynamic processes in living cells. Proc. Natl. Acad. Sci. USA 96:7950-7955.
196. Uster, P. S., and Pagano, R. E. (1986). Resonance energy transfer microscopy: observations of membrane-bound fluorescent probes in model membranes and in living cells. J. Cell Biol. 103:1221-1234.
197. Varnai, P., Rother, K. I., and Balla, T. (1999). Phosphatidylinositol 3-kinase-dependent membrane association of the Bruton's tyrosine kinase pleckstrin homology domain visualized in single living cells. J. Biol. Chem. 274:10983-10989.
198. Vasudevan, C., Han, W., Tan, Y., Nie, Y., Li, D., Shome, K., Watkins, S. C., Levitan, E. S., and Romero, G. (1998). The distribution and translocation of the G protein ADP-ribosylation factor 1 in live cells is determined by its GTPase activity. J. Cell Sci. 111:1277-1285.
199. Walter, J., Schermelleh, L., Cremer, M., Tashiro, S., and Cremer, T. (2003). Chromosome order in HeLa cells changes during mitosis and early Gl, but is stably maintained during subsequent interphase stages. J. Cell Biol. 160:685-697.
200. Wang, S., and Hazelrigg, T. (1994). Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis. Nature 369:400-403.
201. Ward, B. M., and Moss, B. (2001). Vaccinia virus intracellular movement is associated with microtubules and independent of actin tails. J. Virol. 75:11651-11663.
202. Ward, T. H., Polishchuk, R. S., Caplan, S., Hirschberg, K., and Lippincott-Schwartz, J. (2001). Maintenance of Golgi structure and function depends on the integrity of ER export. J. Cell Biol. 155:557-570.
203. Watanabe, N., and Mitchison, T. J. (2002). Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science 295:1083-1086.
204. Waterman-Storer, C. M., and Danuser, G. (2002). New directions for fluorescent speckle microscopy. Curr. Biol. 12:R633-R640.
205. Waterman-Storer, C. M., Desai, A., Bulinski, J. C., and Salmon, E. D. (1998). Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells. Curr. Biol. 8:1227-1230.
206. Weiss, M., and Nilsson, T. (2003). A kinetic proof-reading mechanism for protein sorting. Traffic 4:65-73.
207. Weiss, M., and Nilsson, T. (2004). In a mirror dimly: tracing the movements of molecules in living cells. Trends Cell Biol. 14:267-273.
208. Weiss, M., Hashimoto, H., and Nilsson, T. (2003). Anomalous protein diffusion in living cells as seen by fluorescence correlation spectroscopy. Biophys. J. 84:4043-4052.
209. White, J., Keller, P., and Stelzer, E. H. (2001). Spatial partitioning of secretory cargo from Golgi resident proteins in live cells. BMC Cell Biol. 2:19.
210. White, J., and Stelzer, E. (1999). Photobleaching GFP reveals protein dynamics inside live cells. Trends Cell Biol. 9:61-65.
211. Wiegand, U. K., Duncan, R. R., Greaves, J., Chow, R. H., Shipston, M. J., and Apps, D. K. (2003). Red, yellow, green go! A novel tool for microscopic segregation of secretory vesicle pools according to their age. Biochem. Soc. Trans. 31:851-856.
212. Wiseman, P. W., Brown, C. M., Webb, D. J., Hebert, B., Johnson, N. L., Squier, J. A., Ellisman, M. H., and Horwitz, A. F. (2004). Spatial mapping of integrin interactions and dynamics during cell migration by Image Correlation Microscopy. J. Cell Sci. 117:5521-5534.
213. Wouters, F. S., and Bastiaens, P. I. (1999). Fluorescence lifetime imaging of receptor tyrosine kinase activity in cells. Curr. Biol. 9:1127-1130.
214. Wu, P., and Brand, L. (1994). Resonance energy transfer: Methods and applications. Anal. Biochem. 218:1-13.
215. Wu, X., Rao, K., Bowers, M. B., Copeland, N. G., Jenkins, N. A., and Hammer, J. A., III (2001a). Rab27a enables myosin Va-dependent melanosome capture by recruiting the myosin to the organelle. J. Cell Sci. 114:1091-1100.
216. Wu, X., Zhao, X., Baylor, L., Kaushal, S., Eisenberg, E., and Greene, L. E. (2001b). Clathrin exchange during clathrin-mediated endocytosis. J. Cell Biol. 155:291-300.
217. Yang, T. T., Cheng, L., and Kain, S. R. (1996a). Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein. Nucleic Acids Res. 24:4592-1593.
218. Yang, T. T., Kain, S. R., Kitts, P., Kondepudi, A., Yang, M. M., and Youvan, D. C. (1996b). Dual color microscopic imagery of cells expressing the green fluorescent protein and a red-shifted variant. Gene 173:19-23.
219. Zaal, K. J. M., Smith, C. L., Polishchuk, R. S., Altan, N., Cole, N. B., Ellenberg, J., Hirschberg, K., Presley, J. F., Roberts, T. H., Siggia, E., Phair, R. D., and Lippincott-Schwartz, J. (1999). Golgi membranes are absorbed into and reemerge from the ER during mitosis. Cell 99:589-601.
220. 2+ signals. Curr. Opin. Cell Biol. 14:160-166.
221. Zacharias, D. A., Violin, J. D., Newton, A. C., and Tsien, R. Y. (2002). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296:913-916.
222. Zamir, E., Katz, M., Posen, Y., Erez, N., Yamada, K. M., Katz, B. Z., Lin, S., Lin, D. C., Bershadsky, A., Kam, Z., and Geiger, B. (2000). Dynamics and segregation of cell-matrix adhesions in cultured fibroblasts. Nat. Cell Biol. 2:191-196.
223. Zhang, G., Gurtu, V., and Kain, S. R. (1996). An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem. Biophys. Res. Commun. 227:707-711.
224. Zhang, J., Campbell, R. E., Ting, A. Y., and Tsien, R. Y. (2002). Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3:906-918.
225. Zhang, G.-J., Safran, M., Wei, W., Sorensen, E., Lassota, P., Zhelev, N., Neuberg, D. S., Shapiro, G., and Kaelin, W. G., Jr. (2004). Bioluminescent imaging of Cdk2 inhibition in vivo. Nat. Med. 10:643-648.
226. Zolotukhin, S., Potter, M., Hauswirth, W. W., Guy, J., and Muzyczka, N. (1996). A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells. J. Virol. 70:4646-4654.