Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa: Cloning, over-expression in Escherichia coli, and regulation by choline and salt

Archives of Microbiology

Volumn 185, Issue 1, 2006, Pages 14-22

  Velasco García, Roberto ^a   Villalobos, Miguel Angel ^b   Ramírez Romero, Miguel A ^b   Mújica Jiménez, Carlos ^b   Iturriaga, Gabriel ^c   Muñoz Clares, Rosario A ^b  

^a UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^b UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^c UNIVERSIDAD AUTÓNOMA DEL ESTADO DE MORELOS   (Mexico)

Author keywords

Bet system; Betaine aldehyde dehydrogenase; Choline induction; Computational promoter analysis; Pseudomonas aeruginosa; Salt inhibition

Indexed keywords

AEROBIC RESPIRATION CONTROL PROTEIN A; ALDEHYDE DEHYDROGENASE; BETAINE; BETAINE ALDEHYDE DEHYDROGENASE; CHOLINE; GLUCOSE; LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN; RECOMBINANT PROTEIN; SODIUM CHLORIDE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR BET1; TRYPTOPHAN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL CELL; BINDING SITE; CELL ACTIVITY; CELL GROWTH; CIRCULAR DICHROISM; ESCHERICHIA COLI; FLAME PHOTOMETRY; FLUORESCENCE; GEL PERMEATION CHROMATOGRAPHY; IN VIVO STUDY; INFECTION; KINETICS; MATHEMATICAL ANALYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; OSMOLARITY; OSMOTIC STRESS; PRIORITY JOURNAL; PROMOTER REGION; PROTECTION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PSEUDOMONAS AERUGINOSA; REGULATORY MECHANISM; SALT TOLERANCE; WESTERN BLOTTING;

BASE SEQUENCE; BETAINE-ALDEHYDE DEHYDROGENASE; CHOLINE; ESCHERICHIA COLI; GLUCOSE; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; PSEUDOMONAS AERUGINOSA; RECOMBINANT PROTEINS; SODIUM CHLORIDE;

ARCA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS AERUGINOSA;

EID: 33644533183     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00203-005-0054-8     Document Type: Article

References (40)

1. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-3402
2. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1989) Short protocols in molecular biology. Wiley, New York
3. Boch J, Kempf B, Schimd R, Bremer E (1996) Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes. J Bacteriol 178:5121-5129
4. Boch J, Nau-Wagner G, Kneip S, Bremer E (1997) Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine. Arch Microbiol 168:282-289
5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-256
6. Brinkman AB, Ettema TJ, de Vos WM, van der Oost J (2003) The Lrp family of transcriptional regulators. Mol Microbiol 48:287-94
7. Csonka LN (1989) Physiological and genetic responses of bacteria to osmotic stress. Microbiol Rev 53:121-147
8. D'souza-Ault MR, Smith LT, Smith GM (1993) Roles of N-acetylglutaminyl- glutamine amide and glycine betaine in adaptation of Pseudomonas aeruginosa to osmotic stress. Appl Environ Microbiol 59:473-478
9. Falkenberg P, Strøm AR (1990) Purification and characterization of osmoregulatory betaine aldehyde dehydrogenase from Escherichia coli. Biochim Biophys Acta 1034:253-259
10. Guzmán-Partida A, Valenzuela-Soto EM (1998) Porcine kidney betaine aldehyde dehydrogenase: purification and properties. Comp Biochem Physiol 119:485-491
11. Hibino T, Meng YL, Kawamitsu Y, Uehara N, Matsuda N, Tanaka Y, Ishikawa H, Baba S, Takabe T, Wada K, Ishii T, Takabe T (2001) Molecular cloning and functional characterization of two kinds of betaine-aldehyde dehydrogenase in betaine-accumulating mangrove Avicennia marina (Forsk.) Vierh. Plant Mol Biol 45:353-363
12. Incharoensakdi A, Matsuda N, Hibino T, Meng YL, Ishikawa H, Hara A, Funaguma T, Takabe T, Takabe T (2000) Overproduction of spinach betaine aldehyde dehydrogenase in Escherichia coli. Structural and functional properties of wild-type, mutants and E. coli enzymes. Eur J Biochem 267:7015-7023
13. Kempf B, Bremer E (1998) Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch Microbiol 170:319-330
14. Kilbourn JP (1978) Bacterial content and ionic composition of sputum in cystic fibrosis. Lancet 311:334
15. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 227:680-685
16. Lamark T, Kaasen EM, Eshoo W, Falkenberg P, McDougall J, Strøm AR (1991) DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli. Mol Microbiol 5:1049-1064
17. Lamark T, Røkenes TP, Mcdougall J, Strøm AR (1996) The complex bet promoters of Escherichia coli: regulation by oxygen (ArcA), choline (BetI), and osmotic stress. J Bacteriol 178:1655-1662
18. Levering PR, van Dijken JP, Veenhius M, Harder W (1981) Arthrobacter P1, a fast growing versatile methylotroph with amine oxidase as a key enzyme in the metabolism of methylated amines. Arch Microbiol 129:72-80
19. Lisa TA, Casale CH, Domenech CE (1994) Cholinesterase, acid phosphatase, and phospholipase C of Pseudomonas aeruginosa under hyperosmotic conditions in a high-phosphate medium. Curr Microbiol 28:71-76
20. Mandon K, Østerås M, Boncompagni E, Trinchant JC, Spennato G, Poggi MC, Le Redulier D (2003) The Sinorhizobium meliloti glycine betaine biosynthetic genes (betICBA) are induced by choline and highly expressed in bacteroids. Mol Plant Microbe Interact 16:709-719
21. Meskys R, Harris RJ, Casaite V, Basran J, Scruton NS (2001) Organization of the genes involved in dimethylglycine and sarcosine degradation in Arthrobacter spp. Implications for glycine betaine catabolism. Eur J Biochem 268:3390-3398
22. Miller JH (1977) Experiments in molecular genetics, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 431-435
23. Nagasawa T, Kawabata Y, Tani Y, Ogata. K (1976) Purification and characterization of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa A-16. Agric Biol Chem 40:1743-1749
24. Ostroff RM, Wretlind B, Vasil ML (1989) Identification of a new phospholipase C activity by analysis of an insertional mutation in the hemolytic phospholipase C structural gene of Pseudomonas aeruginosa. J Bacteriol 169:4597-4601
25. Pesin SR, Candia OA (1982) Acetylcholine concentration and its role in ionic transport by the corneal epithelium. Invest Ophtalmol Vis Sci 22:651-659
26. Pocard J-A, Vincent N, Boncompagni E, Smith LT, Poggi MC, Le Rudulier D (1997) Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34. Microbiology 143:1369-1379
27. Rathinasabapathi B, McCue KF, Gage DA, Hanson AD (1996) Metabolic engineering of glycine betaine synthesis: plant betaine aldehyde dehydrogenases lacking typical transit peptides are targeted to tobacco chloroplasts where they confer betaine aldehyde resistance. Planta 193:155-152
28. Rennick BR (1981) Renal tubule transport of organic ions. Am J Physiol 240:F83-F89
29. Røkenes TP, Lamark T, Strøm AR (1996) DNA-binding properties of the BetI repressor protein of Escherichia coli: the inducer choline stimulates BetI-DNA complex formation. J Bacteriol 178:1663-1670
30. Sage AE, Vasil AI, Vasil ML (1997) Molecular characterization of mutants affected in the osmoprotectant-dependent induction of phospholipase C in Pseudomonas aeruginosa. Mol Microbiol 23:43-56
31. Serra AL, Mariscotti JF, Barra JL, Lucchesi GI, Domenech CE, Lisa AT (2002) Glycine betaine transmethylase mutant of Pseudomonas aeruginosa. J Bacteriol 184:4301-4303
32. Shortridge VD, Lazdunski A, Vasil ML (1992) Osmoprotectants and phosphate regulate expression of phospholipase C in Pseudomonas aeruginosa. Mol Microbiol 6:863-871
33. Smith LT, Pocard JA, Bernard T, Le Rudulier D (1988) Osmotic control of glycine betaine biosynthesis and degradation in Rhizobium meliloti. J Bacteriol 170:3142-3149
34. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4353
35. Valenzuela-Soto EM, Muñoz-Clares RA (1994) Purification and properties of betaine aldehyde dehydrogenase extracted from detached leaves of Amaranthus hypochondriacus L. subjected to water deficit. J Plant Physiol 143:145-152
36. Valenzuela-Soto EM, Velasco-Garcia R, Mújica-Jiménez C, Muñoz-Clares RA. (2003) Monovalent cations requirements for the stability of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa, amaranth leaves and porcine kidney. ChemBiol Interact 143-144:139-148
37. +-dependent reactions catalyzed by betaine aldehyde dehydrogenase from Pseudomonas aeruginosa. Biochem J 352:675-683
38. Velasco-García R., Mújica-Jiménez C, Mendoza-Hernández G, Muñoz-Clares RA (1999) Rapid purification and properties of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa. J Bacteriol 181:1292-1300
39. Welsh DT (2000) Ecological significance of compatible solute accumulation by micro-organisms: From single cells to global climate. FEMS Microbiol Rev 24:263-290
40. Wright JR, Clements JA (1987) Metabolism and turnover of lung surfactant. Am Rev Respir Dis 136:426-444