Novel polymorphisms in mec genes and a new mec complex type in methicillin-resistant Staphylococcus aureus isolates obtained in rural Wisconsin

Antimicrobial Agents and Chemotherapy

Volumn 48, Issue 8, 2004, Pages 3080-3085

  Shukla, Sanjay K ^a   Ramaswamy, Srinivas V ^b   Conradt, Jennifer ^a   Stemper, Mary E ^a   Reich, Robert ^b   Reed, Kurt D ^a   Graviss, Edward A ^b  

^a MARSHFIELD CLINIC   (United States)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL DNA; CYTOSINE DERIVATIVE; METICILLIN; OXACILLIN;

ALLELISM; AMINO ACID SEQUENCE; ARTICLE; BACTERIUM ISOLATION; DRUG USE; GENE; GENE DELETION; GENE ISOLATION; GENE SEQUENCE; GENETIC POLYMORPHISM; GENETIC SUSCEPTIBILITY; MEC GENE; MECA GENE; MECI GENE; MECR1 GENE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; RURAL AREA; STAPHYLOCOCCUS AUREUS; STRAIN IDENTIFICATION; UNITED STATES;

ALLELES; DNA PRIMERS; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; EPIDEMIOLOGY, MOLECULAR; GENES, BACTERIAL; HUMANS; METHICILLIN RESISTANCE; MICROBIAL SENSITIVITY TESTS; MUTATION; POLYMORPHISM, GENETIC; PROMOTER REGIONS (GENETICS); REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; WISCONSIN;

EID: 3342960500     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.48.8.3080-3085.2004     Document Type: Article

References (18)

1. Archer, G. L., and J. M. Bosilevac. 2001. Signaling antibiotic resistance in staphylococci. Science 291:1915-1916.
2. Berger-Bachi, B., and S. Rohrer. 2002. Factors influencing methicillin resistance in staphylococci. Arch. Microbiol. 178:165-171.
3. Chambers, H. F., and M. Sachdeva. 1990. Binding of beta-lactam antibiotics to penicillin-binding proteins in methicillin-resistant Staphylococcus aureus. J. Infect. Dis. 161:1170-1176.
4. Hiramatsu, K. 1995. Molecular evolution of MRSA. Microbiol. Immunol. 39:531-543.
5. Ito, T., Y. Katayama, and K. Hiramatsu. 1999. Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrob. Agents Chemother. 43:1449-1458.
6. Katayama, Y., T. Ito, and K. Hiramatsu. 2001. Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: role of IS431-mediated mecI deletion in expression of resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus. Antimicrob. Agents Chemother. 45:1955-1963.
7. Katayama, Y., H.-Z. Zhang, and H. F. Chambers. 2004. PBP 2a mutations producing very-high-level resistance to beta-lactams. Antimicrob. Agents Chemother. 48:453-459.
8. Kobayashi, N., K. Taniguchi, K. Kojlma, S. Urasawa, N. Uehara, Y. Omizu, Y. Kishi, A. Yagihashi, I. Kurokawa, and N. Watanabe. 1996. Genomic diversity of mec regulator genes in methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Epidemiol. Infect. 117:289-295.
9. Kobayashi, N., K. Taniguchi, and S. Urasawa. 1998. Analysis of diversity of mutations in the mecI gene and mecA promoter/operator region of methicillin-resistant Staphylococcus attreus and Staphylococcus epidermidis. Antimicrobiol. Agents Chemother. 42:717-720.
10. Kuroda, M., T. Ohta, I. Uchiyama, T. Baba, H. Yuzawa, I. Kobayashi, L. Cui, A. Oguchi, K. Aoki, Y. Nagai, J. Lian, T. Ito, M. Kanamori, H. Matsumaru, A. Maruyama, H. Murakami, A. Hosoyama, Y. Mizutani-Ui, N. K. Takahashi, T. Sawano, R. Inoue, C. Kaito, K. Sekimizu, H. Hirakawa, S. Kuhara, S. Goto, J. Yabuzaki, M. Kanehisa, A. Yamashita, K. Oshima, K. Furuya, C. Yoshino, T. Shiba, M. Hattori, N. Ogasawara, H. Hayashi, and K. Hiramatsu. 2001. Whole genome sequencing of methicillin-resistant Staphylococcus aureus. Lancet 357:1225-1240.
11. Lim, T. T., G. W. Coombs, and W. B. Grubb. 2002. Genetic organization of mecA and mecA-regulatory genes in epidemic methicillin-resistant Staphylococcus aureus from Australia and England. J. Antimicrob. Chemother. 50:819-824.
12. McKinney, T. K., V. K. Sharma, W. A. Craig, and G. L. Archer. 2001. Transcription of the gene mediating methicillin resistance in Staphylococcus aureus (mecA) is corepressed but not coinduced by cognate mecA and β-lactamase regulators. J. Bacteriol. 183:6862-6868.
13. National Committee for Clinical Laboratory Standards. 1999. Standard M100-S9. Performance standards for antimicrobial susceptibility testing, 9th supplement, vol. 19. National Committee for Clinical Laboratory Standards, Wayne, Pa.
14. Petinaki, E., A. Arvaniti, G. Dimitracopoulos, and I. Spiliopoulou. 2001. Detection of mecA, mecR1, and mecI genes among clinical isolates of methicillin-resistant staphylococci by combined polymerase chain reactions. J. Antimicrob. Chemother. 47:297-304.
15. Rosato, A. E., W. A. Craig, and G. L. Archer. 2003. Quantitation of mecA transcription in oxacillin-resistant Staphylococcus aureus clinical isolates. J. Bacteriol. 185:3446-3452.
16. Suzuki, E., K. Kuwahara-Arai, J. F. Richardson, and K. Hiramatsu. 1993. Distribution of mec regulator genes in methicillin-resistant Staphylococcus clinical strains. Antimicrob. Agents Chemother. 37:1219-1226.
17. Vandenesch, F., T. Naimi, M. C. Enright, G. Lina, G. R. Nimmo, H. Heffernan, N. Liassine, M. Bes, T. Greenland, M. E. Reverdy, and J. Etienne. 2003. Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwide emergence. Emerg. Infect. Dis. 9:978-984.
18. Weller, T. M. 1999. The distribution of mecA, mecR1 and mecI and sequence analysis of mecI and the mec promoter region in staphylococci expressing resistance to methicillin. J. Antimicrob. Chemother. 43:15-22.