Biophysical and kinetic analysis of wild-type and site-directed mutants of the isolated and native dehydroquinate synthase domain of the AROM protein

Protein Science

Volumn 13, Issue 8, 2004, Pages 2108-2119

  Park, Alison ^a   Lamb, Heather K ^a   Nichols, Charlie ^b   Moore, Jonathan D ^a,f   Brown, Katherine A ^c   Cooper, Alan ^d   Charles, Ian G ^e   Stammers, David K ^b   Hawkins, Alastair R ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

^d UNIVERSITY OF GLASGOW   (United Kingdom)

^e Arrow Therapeutics   (United Kingdom)

^f Vernalis PLC   (United Kingdom)

Author keywords

AROM; Biocalorimetry; Dehydroquinate synthase; Site directed mutagenesis

Indexed keywords

3 DEOXY DEXTRO ARABINOHEPTULOSONATE 7 PHOSPHATE; DEHYDROQUINATE; DEHYDROQUINATE SYNTHASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; PHOSPHATE; PROTEIN; PROTEIN AROM; QUININE SULFATE; SHIKIMIC ACID; SYNTHETASE; UNCLASSIFIED DRUG;

ALCOHOL OXIDATION; ALDOL REACTION; ARTICLE; BINDING SITE; BIOPHYSICS; CALORIMETRY; CATALYSIS; CHEMICAL MODIFICATION; CHEMICAL REACTION; CHROMATOGRAPHY; CIRCULAR DICHROISM; DIFFERENTIAL SCANNING CALORIMETRY; EUKARYOTE; KINETICS; LIGAND BINDING; METAL BINDING; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN QUATERNARY STRUCTURE; PROTEIN SECONDARY STRUCTURE; REDUCTION; RING OPENING; SITE DIRECTED MUTAGENESIS; WILD TYPE;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; BIOPHYSICS; CALORIMETRY, DIFFERENTIAL SCANNING; HUMANS; HYDRO-LYASES; KINETICS; LYASES; MULTIENZYME COMPLEXES; MUTAGENESIS, SITE-DIRECTED; PHOSPHORUS-OXYGEN LYASES; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PROTEIN STRUCTURE, TERTIARY; QUINIC ACID; SUBSTRATE SPECIFICITY; SUGAR PHOSPHATES; TRANSFERASES;

CINCHONA PUBESCENS; EUKARYOTA;

EID: 3342931053     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.04705404     Document Type: Article

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