Computing TaqMan probes for multiplex PCR detection of E. coli O157 serotypes in water

In Silico Biology

Volumn 5, Issue 5-6, 2005, Pages 499-504

  Ram, Siya ^a   Shanker, Rishi ^a  

^a CSIR INDIAN INSTITUTE OF TOXICOLOGY RESEARCH   (India)

Author keywords

E. coli O157; Polymerase chain reaction; TaqMan probe

Indexed keywords

OLIGOMER;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM DETECTION; COMPUTER MODEL; COMPUTER PROGRAM; ESCHERICHIA COLI O157; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC CONSERVATION; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SEROTYPE; UNINDEXED SEQUENCE; VALIDATION PROCESS; WASTE WATER MANAGEMENT; WATER CONTAMINATION; WATER QUALITY;

BASE SEQUENCE; DNA PRIMERS; DNA PROBES; DNA, BACTERIAL; ESCHERICHIA COLI O157; GENES, BACTERIAL; HUMANS; MOLECULAR PROBE TECHNIQUES; POLYMERASE CHAIN REACTION; SEROTYPING; VIRULENCE; WATER MICROBIOLOGY;

EID: 33244484375     PISSN: 13866338     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Brandon, H. (1995). The Cost of Inaction: Valuing the Economy-wide Cost of Environmental Degradation in India. World Bank Report. World Bank, New York.
2. D'Souza, R. (2003). Colored Water: Mystery Solved. Times News Network. The Times of India (Issue: April 24), Lucknow, India.
3. Frahm, E. and Obst, U. (2003). Applications of the fluorogenic probe technique (TaqMan PCR) to detection of Enterococcus spp. and Escherichia coli in water sample. J. Microbiol. Methods 52, 123-131.
4. Gavin, P. J. and Thomson, R. B. (2004). Diagnosis of enterohemorrhagic Escherichia coli infection by detection of shiga toxins. Clin. Microbiol. Newsl. 26, 49-54.
5. Johnson, I. and Lvovsky, K. (2001). Indoor air pollution: Energy and Health for the Poor. World Bank Special: Double Burden. World Bank/ESMAP Newsletter Issue no. 3, February 2001.
6. Karch, H. and Bielaszewska, M. (2001). Sorbitol-fermenting shiga toxin-producing Escherichia coli O157: H- strains: Epidemiology, phenotypic and molecular characteristics, and microbiological diagnosis. J. Clin. Microbiol. 39, 2043-2049.
7. Lekowska-Kochaniak, A., Czajkowska, D. and Popowski, J. (2002). Detection of Escherichia coli O157:H7 in raw meat by immunomagnetic separation and multiplex PCR. Acta Microbiol. Pol. 51, 327-337.
8. Linssen, B., Kinney, R. M., Aguilar, P., Russell, K. L., Watts, D. M., Kaaden, O. R. and Pfeffer, M. (2000). Development of reverse transcription-PCR assay specific for detection of equine encephalitis viruses. J. Clin. Microbiol. 38, 1527-1535.
9. Mead, P. S. and Griffin, P. M. (1998). Escherichia coli O157:H7. Lancet 352, 1207-1212.
10. Jothikumar, N. and Griffiths, M. W. (2002). Detection of Escherichia coli O157:H7 with multiplex real-time PCR Assays. Appl. Environ. Microbiol. 68, 3169-3171.
11. Weinberger, K. M., Wiedenmann, E., and Jilg, W. (2000). Sensitive and accurate quantitation of hepatitis B viruses DNA using a kinetic fluorescence detection system (TaqMan PCR). J. Virol. Methods 85, 75-82.
12. Zhang, W.-L., Bielaszewska, M., Liesegang, A., Tschape, H., Schmidt, H., Bitzan, M. and Karch, H. (2000). Molecular characteristics and epidemiological significance of Shiga toxin-producing Escherichia coli O26 strains. J. Clin. Microbiol. 38, 2134-2140.